The neuroprotective properties and therapeutic potential of epidermal neural crest stem cells transplantation in a rat model of vascular dementia

Akbari, Sara; Hooshmandi, Etrat; Bayat, Mahnaz; Haghighi, Afshin Borhani; Salehi, Mohammad Saied; Pandamooz, Sareh; Nejad, Amirhossein Yousefi; Haghani, Masoud

doi:10.1016/j.brainres.2021.147750

Cited by 10 publications

(19 citation statements)

References 63 publications

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“…We performed the allograft transplantation of 1 million ESC without immunosuppression was repeated three times after 2VO surgery. In our previous study, the transplantation of 1 million cells showed better functional recovery in the 2VO rats to 2.5 million cell grafting 11 .…”

Section: Discussionmentioning

confidence: 75%

“…Perhaps the synergistic effect of combination therapy in releasing the growth factors on the host neural circuits caused this functional improvement without any new circuits between the host and grafted cells. Previous reports have suggested the hippocampal synaptic plasticity improvement following stem cell transplantation in 2VO and myocardial infarction models 11,31 . It has been indicated that EPI-NCSCs could express the vascular endothelial growth factor (VEGF), Neurotrophic growth factor (NGF), and Brain-derived neurotrophic factor (BDNF), [32][33][34] .…”

Section: Discussionmentioning

confidence: 90%

“…In our study, there was no report of animal death following cell transplantation. The different methods, such as surgery, cell transplantation, behavioral tests, PRP injection, eld potential recording, and Real-time PCR, were performed according to our previously published study 11 (Fig. 1).…”

Section: Experimental Groupsmentioning

confidence: 99%

“…In-vitro studies have reported that epidermal neural crest stem cells (EPI-NCSC) of hair follicles could differentiate into neural cells, oligodendrocytes, astrocytes, and Schwann cells 9,10 . Our previous study found that the intravenous infusion of 1×10 6 EPI-NCSC performed better than the 2.5×10 6 cells in the tow-vessels occlusion (2VO) rats 11 . Additionally, in-vivo studies have shown the migration potential of EPI-NCSC to the injury side, enhanced functional recovery in different animal models of neurodegenerative diseases such as ischemic stroke 5 , Alzheimer's diseases 12 , peripheral nerve injury 13 , and spinal cord injury 14 .…”

Section: Introductionmentioning

confidence: 97%

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Combination therapy with platelet-rich plasma and epidermal neural crest stem cells increases treatment efficacy in vascular dementia

Akbari

Haghani

Ghobadi

et al. 2023

Preprint

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This study aimed to evaluate effects of platelet-rich plasma (PRP) and neural crest-derived epidermal stem cells (ESC) in alone administration and their combination in vascular dementia (VaD) model by tow-vessels occlusion (2VO). Methods the rats were divided into control, sham, 2VO + vehicle, 2VO + PRP, 2VO + ESC, and 2VO + ESC + PRP groups. The treated-groups received one million cells on days 4, 14, and 21 with or without 500µl PRP (twice a week). The behavioral performance was evaluated by open-field, passive-avoidance, and Morris-water maze. The basal-synaptic transmission (BST) and long-term potentiation (LTP) were assessed by field-potential recording. The mRNA expression levels of IGF-1, TGF-β1, PSD-95, and GSk-3β were measured in rat hippocampus by qRT-PCR. Results The results showed a learning, memory, and synaptic plasticity impairment with a significant decrease in IGF1, TGF-β1, PSD-95, and increase in expression of GSk-3β 2VO rats. Although treatment with ESC alone and ESC + PRP show comparable improvement effects on spatial memory and LTP induction associated with PSD-95 up-regulation and GSK-3β down-regulation, the BST only recovered in the ESC + PRP group. Moreover, combination therapy was superior to monotherapy with PRP. Conclusions The transplantation of ESC showed better effects than PRP alone, and combination therapy increased treatment efficacy with the recovery of BST.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 90%

Section: Experimental Groupsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Combination therapy with platelet-rich plasma and epidermal neural crest stem cells increases treatment efficacy in vascular dementia

Akbari

Haghani

Ghobadi

et al. 2023

Preprint

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“…Isolation of hair follicles from the skin does not raise ethical concerns and by autologous transplantation, immunological graft rejection would be avoided. In addition, up to now, no immunological rejection are reported following allograft or xenograft transplantation of HFSCs in several nervous system conditions such as Alzheimer’s disease [ 10 ], vascular dementia [ 11 ], peripheral nerve injury [ 12 ], also animals [ 13 ] and organotypic [ 14 , 15 ] models of spinal cord injury. Moreover, HFSCs express a wide range of extracellular proteases, angiogenic factors, and growth factors that eventually may lead to neuroprotection and neo-vascularization [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of hair follicle-derived stem cell intranasal transplantation in a rat model of ischemic stroke

et al. 2022

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Background Stem cell-based therapy has received considerable attention as a potential candidate in the treatment of ischemic stroke; however, employing an appropriate type of stem cells and an effective delivery route are still challenging. In the present study, we investigated the therapeutic effect of safe, noninvasive, and brain-targeted intranasal administration of hair follicle-derived stem cells (HFSCs) in a rat model of ischemic stroke. Methods Stem cells were obtained from the adult rat hair follicles. In experiment 1, stroke was induced by 30 min middle cerebral artery occlusion (MCAO) and stem cells were intranasally transplanted immediately after ischemia. In experiment 2, stroke was induced by 120 min MCAO and stem cells were administered 24 h after cerebral ischemia. In all experimental groups, neurological performance, short-term spatial working memory and infarct volume were assessed. Moreover, relative expression of major trophic factors in the striatum and cortex was evaluated by the quantitative PCR technique. The end point of experiment 1 was day 3 and the end point of experiment 2 was day 15. Results In both experiments, intranasal administration of HFSCs improved functional performance and decreased infarct volume compared to the MCAO rats. Furthermore, NeuN and VEGF expression were higher in the transplanted group and stem cell therapy partially prevented BDNF and neurotrophin-3 over-expression induced by cerebral ischemia. Conclusions These findings highlight the curative potential of HFSCs following intranasal transplantation in a rat model of ischemic stroke.

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Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia

Ghobadi,

Akbari,

Bayat

et al. 2023

Brain and Behavior

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IntroductionVascular dementia (VaD) is a common type of dementia. The aim of this study was to investigate the cellular and molecular mechanism of conditioned medium (CM) in VaD.Material and methodsThe rats were divided into four groups of control (n = 9), sham‐operation (n = 10), VaD with vehicle (n = 9), and VaD with CM (n = 12) that received CM on days 4, 14, and 24 after 2VO. Before sacrificing the rats, cognitive performance was assessed through the open‐field (OP), passive‐avoidance, and Morris‐water maze. The field‐potential recording was used to investigate basal synaptic transmission (BST) and long‐term potentiation (LTP). Subsequently, the hippocampus was dissected, and real‐time PCR was used to quantify the expression levels of β1‐catenin, insulin‐like growth factor‐1 (IGF‐1), transforming growth factor‐beta (TGF‐β), glycogen synthase kinase‐3β (GSK‐3β), postsynaptic density protein 95 (PSD‐95), and NR2B genes.ResultsThe results indicated impaired performance in behavioral tests in 2VO rats, coupled with reductions in BST and LTP induction. The expression levels of β1‐catenin, IGF‐1, PSD‐95, and TGF‐β genes decreased, whereas NR2B and GSK‐3β expression increased. Treatment with CM restores the expression of PSD‐95 and GSK‐3β as well as fear‐memory, spatial learning, and grooming number without a positive effect on memory retrieval, time spent on the periphery and center of OP. The BST recovered upon administration of CM but, the LTP induction was still impaired.ConclusionThe recovery of BST in VaD rats appears to be the most important outcome of this study which is caused by the improvement of gene expression and leads to the restoration of fear memory.

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The neuroprotective properties and therapeutic potential of epidermal neural crest stem cells transplantation in a rat model of vascular dementia

Cited by 10 publications

References 63 publications

Combination therapy with platelet-rich plasma and epidermal neural crest stem cells increases treatment efficacy in vascular dementia

Combination therapy with platelet-rich plasma and epidermal neural crest stem cells increases treatment efficacy in vascular dementia

Therapeutic potential of hair follicle-derived stem cell intranasal transplantation in a rat model of ischemic stroke

Gens PSD‐95 and GSK‐3β expression improved by hair follicular stem cells‐conditioned medium enhances synaptic transmission and cognitive abilities in the rat model of vascular dementia

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