The neuroprotective role of morroniside against spinal cord injury in female rats

Duan, Fei‐Xiang; Shi, Yujiao; Chen, Jing; Xue, Song; Shen, Lin; Qi, Qi; Ding, Songtao; Wang, Qiyi; Wang, Rui; Lü, He‐Zuo; Hu, Jianguo

doi:10.1016/j.neuint.2021.105105

Cited by 17 publications

(12 citation statements)

References 43 publications

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“…Nine cross‐sectional images were collected from the lesion epicenter (the section containing the largest lesion area and the least sparing of white matter), and light microscopy was used to visualize the sections 1, 2, 3, and 4 mm rostral and caudal to the epicenter. The lesion area and medullary white matter area were measured using ImageJ software (National Institutes of Health) and Image‐Pro 5.1 (Media Cybernetics), respectively, and normalized to the percentage of the entire stained area in an unbiased stereological manner for quantitative analysis 16 . Surviving neurons were quantified by counting the number of Nissl bodies.…”

Section: Methodsmentioning

confidence: 99%

“…Luxol fast blue (LFB), hematoxylin-eosin (HE) and Nissl staining (Beyotime Biotechnology, China) were performed according to the manufacturer's instructions to assess the damaged area, the medullary white matter area and the number of remaining motor neurons as previously described. 16 Nine cross-sectional images were collected from the lesion epicenter (the section containing the largest lesion area and the least sparing of white matter), and light microscopy was used to visualize the sections 1, 2, 3, and 4 mm rostral and caudal to the epicenter. The lesion area and medullary white matter area were measured using ImageJ software (National Institutes of Health) and Image-Pro 5.1 (Media Cybernetics), respectively, and normalized to the percentage of the entire stained area in an unbiased stereological manner for quantitative analysis.…”

Section: Histological Analysismentioning

confidence: 99%

“…The lesion area and medullary white matter area were measured using ImageJ software (National Institutes of Health) and Image-Pro 5.1 (Media Cybernetics), respectively, and normalized to the percentage of the entire stained area in an unbiased stereological manner for quantitative analysis. 16 Surviving neurons were quantified by counting the number of Nissl bodies.…”

Section: Histological Analysismentioning

confidence: 99%

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Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization

et al. 2022

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Background Inflammatory reactions induced by spinal cord injury (SCI) are essential for recovery after SCI. Atractylenolide III (ATL‐III) is a natural monomeric herbal bioactive compound that is mainly derived in Atractylodes macrocephala Koidz and has anti‐inflammatory and neuroprotective effects. Objective Here, we speculated that ATL‐III may ameliorate SCI by modulating microglial/macrophage polarization. In the present research, we focused on investigating the role of ATL‐III on SCI in rats and explored the potential mechanism. Methods The protective and anti‐inflammatory effects of ATL‐III on neuronal cells were examined in a rat SCI model and lipopolysaccharide (LPS)‐stimulated BV2 microglial line. The spinal cord lesion area, myelin integrity, and surviving neurons were assessed by specific staining. Locomotor function was evaluated by the Basso, Beattie, and Bresnahan (BBB) scale, grid walk test, and footprint test. The activation and polarization of microglia/macrophages were assessed by immunohistofluorescence and flow cytometry. The expression of corresponding inflammatory factors from M1/M2 and the activation of relevant signaling pathways were assessed by Western blotting. Results ATL‐III effectively improved histological and functional recovery in SCI rats. Furthermore, ATL‐III promoted the transformation of M1 into M2 and attenuated the activation of microglia/macrophages, further suppressing the expression of corresponding inflammatory mediators. This effect may be partly mediated by inhibition of neuroinflammation through the NF‐κB, JNK MAPK, p38 MAPK, and Akt pathways. Conclusion This study reveals a novel effect of ATL‐III in the regulation of microglial/macrophage polarization and provides initial evidence that ATL‐III has potential therapeutic benefits in SCI rats.

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Section: Methodsmentioning

confidence: 99%

Section: Histological Analysismentioning

confidence: 99%

Section: Histological Analysismentioning

confidence: 99%

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Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization

et al. 2022

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“…15 The neuroprotective role of morroniside in spinal cord injury has also been revealed. 16 These studies confirmed that morroniside could serve as a drug for inflammation and oxidative stress-related diseases.…”

Section: Discussionmentioning

confidence: 66%

Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-κB signaling pathways

Zhang

Lai²,

Li³

et al. 2022

Allergol Immunopathol

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Objective: To investigate the effects of morroniside on inflammatory and oxidative stress in lipopolysaccharide (LPS)-induced inflammatory bowel disease (IBD) cell model. Methods: NCM460 cells were treated with 2-, 5-, or 10-μg/mL LPS for 24 h to develop an IBD cell model. MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) colorimet-ric assay was performed to uncover the role of morroniside on the viability of LPS-treated NCM460 cells. Flow cytometry and immunoblot assays were performed to confirm the effects of morroniside on the apoptosis of LPS-treated NCM460 cells. Quantitative polymerase chain reaction and enzyme-linked-immunosorbent serologic assays were performed to confirm the effects of morroniside on inflammatory and oxidative stress by measuring the levels of tumor necrosis factor-α, interleukin-1β, IL-6, superoxide dismutase, malondialdehyde, total antioxi-dant capacity, and myeloperoxidase. In addition, immunoblot and immunofluorescence assays were performed to detect the effects of morroniside on NLRP3 and NF-κB pathways. Results: Monosine attenuated LPS-induced injury of NCM460 cells. Monosine reduced LPS-induced inflammation in NCM460 cells. In addition, morroniside reduced LPS-induced oxidative stress in NCM460 cells. Mechanically, morroniside suppressed NLRP3 and NF-κB pathways, and alleviated LPS-induced inflammatory and oxidative stress in IBD. Conclusion: Morroniside could serve as a promising drug for treating IBD.

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“…Pretreatment with morroniside can reduce the production of ROS, inhibit the expression of apoptosis-related protein (Bax) and increase the expression of the antiapoptotic gene Bcl-2, to block mitochondrion-mediated apoptosis, and ultimately reduce the apoptosis of neuroblastoma cells induced by H 2 O 2 [ 188 ]. Through its antioxidant activity, morroniside exerts neuroprotective effects in spinal cord injury and may be an effective treatment for this condition [ 189 ]. As it regulates hair follicle growth and development, partly through the Wnt/β-catenin signaling pathway, morroniside may be a potential treatment for hair loss [ 190 ].…”

Section: Monoterpenesmentioning

confidence: 99%

Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family

Miclea

2022

IJMS

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Carnivorous plants have fascinated researchers and hobbyists for centuries because of their mode of nutrition which is unlike that of other plants. They are able to produce bioactive compounds used to attract, capture and digest prey but also as a defense mechanism against microorganisms and free radicals. The main purpose of this review is to provide an overview of the secondary metabolites with significant biological activity found in the Sarraceniaceae family. The review also underlines the necessity of future studies for the biochemical characterization of the less investigated species. Darlingtonia, Heliamphora and Sarracenia plants are rich in compounds with potential pharmaceutical and medical uses. These belong to several classes such as flavonoids, with flavonol glycosides being the most abundant, monoterpenes, triterpenes, sesquiterpenes, fatty acids, alkaloids and others. Some of them are well characterized in terms of chemical properties and biological activity and have widespread commercial applications. The review also discusses biological activity of whole extracts and commercially available products derived from Sarraceniaceae plants. In conclusion, this review underscores that Sarraceniaceae species contain numerous substances with the potential to advance health. Future perspectives should focus on the discovery of new molecules and increasing the production of known compounds using biotechnological methods.

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The neuroprotective role of morroniside against spinal cord injury in female rats

Cited by 17 publications

References 43 publications

Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization

Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization

Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-κB signaling pathways

Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family

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