The neurorestorative effect of human amniotic fluid stem cells on the chronic phase of neonatal hypoxic–ischemic encephalopathy in mice

Otani, Toshiro; Ochiai, Daigo; Masuda, H.; Abe, Yushi; Fukutake, Marie; Matsumoto, Tadashi; Miyakoshi, Kei; Tanaka, Mamoru

doi:10.1038/s41390-018-0131-8

Cited by 22 publications

(26 citation statements)

References 38 publications

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“…S2A). In accordance with our previous studies [18,20], spindle-shaped CD117 + cells (i.e., hAFSCs) were expanded (Supporting Information Fig. S2B).…”

Section: Isolation and Characterization Of Hafscssupporting

confidence: 90%

“…AFSCs have been reported to improve and restore cellular function in the injured nervous system by reducing the inflammatory response, stimulating endogenous repair through the recruitment of progenitor cells, and promoting neuronal outgrowth. This was shown using sciatic nerve injury and stroke models , as well as our data using a neonatal hypoxic–ischemic encephalopathy model . However, key paracrine factors that are secreted from AFSCs remain controversial based on these studies.…”

Section: Discussionmentioning

confidence: 58%

“…Quantitative polymerase chain reaction (PCR) was performed in duplicate in a volume of 25 μl per reaction using a 96‐well Bio‐Rad CFX96 Real‐Time PCR System (Bio‐Rad, Richmond, CA). Reaction mixtures included 5 ng of genomic DNA as the template, 0.4 mM of each primer (Thermo Fisher Scientific, Waltham, MA), SYBR Premix Ex Taq II (Tli RNaseH Plus; Takara Bio), and sterile H 2 O . The primer sets are listed in Supporting Information Table S3.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, to determine the expression of trophic factors derived from hAFSCs in rat amniotic fluid, we isolated CD117 + amniotic fluid cells from E19and E21-rat amniotic fluid cells using CD117 MicroBeads (Miltenyi Biotec). [18,20]. The primer sets are listed in Supporting Information Table S3.…”

Section: Analysis Of E21-rat Amniotic Fluid Cellsmentioning

confidence: 99%

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In Utero Amniotic Fluid Stem Cell Therapy Protects Against Myelomeningocele via Spinal Cord Coverage and Hepatocyte Growth Factor Secretion

Abe

Ochiai

Masuda

et al. 2019

Stem Cells Translational Medicine

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Despite the poor prognosis associated with myelomeningocele (MMC), the options for prenatal treatments are still limited. Recently, fetal cellular therapy has become a new option for treating birth defects, although the therapeutic effects and mechanisms associated with such treatments remain unclear. The use of human amniotic fluid stem cells (hAFSCs) is ideal with respect to immunoreactivity and cell propagation. The prenatal diagnosis of MMC during early stages of pregnancy could allow for the ex vivo proliferation and modulation of autologous hAFSCs for use in utero stem cell therapy. Therefore, we investigated the therapeutic effects and mechanisms of hAFSCs‐based treatment for fetal MMC. hAFSCs were isolated as CD117‐positive cells from the amniotic fluid of 15‐ to 17‐week pregnant women who underwent amniocentesis for prenatal diagnosis and consented to this study. Rat dams were exposed to retinoic acid to induce fetal MMC and were subsequently injected with hAFSCs in each amniotic cavity. We measured the exposed area of the spinal cord and hepatocyte growth factor (HGF) levels at the lesion. The exposed spinal area of the hAFSC‐treated group was significantly smaller than that of the control group. Immunohistochemical analysis demonstrated a reduction in neuronal damage such as neurodegeneration and astrogliosis in the hAFSC‐treated group. Additionally, in lesions of the hAFSC‐treated group, HGF expression was upregulated and HGF‐positive hAFSCs were identified, suggesting that these cells migrated to the lesion and secreted HGF to suppress neuronal damage and induce neurogenesis. Therefore, in utero hAFSC therapy could become a novel strategy for fetal MMC. Stem Cells Translational Medicine 2019;8:1170–1179

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“…S2A). In accordance with our previous studies [18,20], spindle-shaped CD117 + cells (i.e., hAFSCs) were expanded (Supporting Information Fig. S2B).…”

Section: Isolation and Characterization Of Hafscssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%

Section: Analysis Of E21-rat Amniotic Fluid Cellsmentioning

confidence: 99%

See 2 more Smart Citations

In Utero Amniotic Fluid Stem Cell Therapy Protects Against Myelomeningocele via Spinal Cord Coverage and Hepatocyte Growth Factor Secretion

Abe

Ochiai

Masuda

et al. 2019

Stem Cells Translational Medicine

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“…; Otani et al. ). In ophthalmology, amniotic membrane is increasingly used for ocular surface reconstruction, including the treatment of persistent epithelial defects and nonhealing corneal ulcers, corneal perforations and descemetoceles, bullous keratopathy, corneal disorders with associated limbal stem cell deficiency, pterygium, conjunctival reconstruction, corneoscleral melts and perforations, and glaucoma surgery.…”

Section: Introductionmentioning

confidence: 97%

A human Amniotic Membrane plug to manage high myopic macular hole associated with retinal detachment

Caporossi

Angelis

Pacini

et al. 2019

Acta Ophthalmologica

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Objective To assess the efficacy of the human amniotic membrane (hAM) to treat (HMMH) associated with retinal detachment (RD). Material and Methods Ten eyes of 10 patients with recurrent HMMH and RD, who had already undergone one or more pars plana vitrectomy (PPV), underwent a PPV with an hAM plug implanted in the macular hole. The initial five patients enrolled were tamponaded with (SO) while the subsequent five patients with 10% octafluoropropane (C3F8). Silicon oil was removed in all five patients 2 months later. No statistical differences were reported between the two groups. Results Final retinal reattachment was achieved in all the patients. BCVA improved from 1.73 logMAR to 0.94 logMAR after 6 months. No adverse events were registered during follow‐up. Conclusion An hAM plug is an efficient substrate to manage HMMH associated with RD resulting in encouraging visual acuity recovery.

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Amniotic fluid and breast milk: a rationale for breast milk stem cell therapy in neonatal diseases

Filler

Chusilp

et al. 2020

Pediatr Surg Int

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The neurorestorative effect of human amniotic fluid stem cells on the chronic phase of neonatal hypoxic–ischemic encephalopathy in mice

Abstract: hAFS induced functional recovery by exerting neurorestorative effects in HIE mice, suggesting that intranasal administration of hAFS could be a novel treatment for HIE, especially in the chronic phase.

Cited by 22 publications

References 38 publications

In Utero Amniotic Fluid Stem Cell Therapy Protects Against Myelomeningocele via Spinal Cord Coverage and Hepatocyte Growth Factor Secretion

In Utero Amniotic Fluid Stem Cell Therapy Protects Against Myelomeningocele via Spinal Cord Coverage and Hepatocyte Growth Factor Secretion

A human Amniotic Membrane plug to manage high myopic macular hole associated with retinal detachment

Amniotic fluid and breast milk: a rationale for breast milk stem cell therapy in neonatal diseases

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