The Neutrophil

Dockrell, David H.; McGrath, Emmet E.; Whyte, Moria K.B.; Sabroe, Ian

doi:10.1007/1-4020-5492-0_3

Cited by 1 publication

(1 citation statement)

References 119 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, other studies have reported the regulation of gene expression in PMNs stimulated with pathogens (Fradin et al, 2007;Ma et al, 2011). The discrepancy between ELISA and qRT-PCR results concerning TNFα detection could be related to either the short half-life of PMNs in culture or most probably, the few RNA they contain due to their high content of dense chromatin (Dockrell et al, 2007). More specifically, the amount of isolated mRNA could be insufficient to detect a low but potentially significant increase in TNFα transcript despite the high sensitivity of the commercial kits.…”

Section: Discussionmentioning

confidence: 95%

Feline polymorphonuclear neutrophils produce pro-inflammatory cytokines following exposure to Microsporum canis

Cambier

Mathy

Baldo

et al. 2013

Veterinary Microbiology

View full text Add to dashboard Cite

The mechanisms involved in the establishment of the specific immune response against dermatophytes remain unknown. Polymorphonuclear neutrophils (PMNs) are recruited early during the infection process and participate in the elimination of dermatophytes. They could therefore be involved in the induction of the immune response during dermatophytoses by producing specific cytokines. The aim of this work was to assess the in vitro cytokine production by feline PMNs exposed to living arthroconidia from the dermatophyte species Microsporum canis or stimulated with either a secreted or a structural component of M. canis, the latter consisting of heat-killed arthroconidia. The levels of specific cytokines produced by PMNs was determined by capture ELISA and/or quantitative RT-PCR. Results showed that PMNs secrete TNFα, IL-1β and IL-8 following exposure to M. canis living arthroconidia and stimulation with both a secreted component and heat-killed arthroconidia. The level of IL-8 mRNA was also increased in PMNs stimulated with M. canis living arthroconidia. In conclusion, infective M. canis arthroconidia induce the production of pro-inflammatory cytokines by feline PMNs that can be activated either by secreted or structural fungal components. Our results suggest that these granulocytes are involved in the initiation of the immune response against M. canis. We would like to thank you for your consideration of our work as well as for your comments and the opportunity you gave us to submit an amended version of our manuscript. We would like also to thank the reviewers for their critical remarks.Our specific answers to comments are as follow:Editor's comments: As an Editor it is not only my duty to assure the scientific quality of manuscripts, but also to make sure that the limited printing space available is used as efficient as possible. In this respect I have to ask you to reduce the length of your Introduction to one page. I have asked this from numerous authors and do not wish to make an exception for your manuscript.The length of the introduction was reduced as requested. Reviewer 1:My main concerns relates to:-some minor typo-errors occurring in the manuscript (i.e., line 258 "Trichomonas vaginalis") that should be corrected during revision.Line 254: "Trichomona vaginalis" was replaced by "Trichomonas vaginalis".-the discussion section, which would benefit from some modification. The content of lines 214-215 is over repeated in this part, so I would ask to the Authors to adjust it throughout the text. The concepts of lines 215-219 should be for other parts of the work.The content of the lines 214-219 was deleted to avoid repetition in the work. Reviewer 2:Revision Note However, a fungal culture was performed and was negative for dermatophytes in all cases.Concerning haematological analyses, a blood smear confirmed that feline PMNs had no morphological abnormalities and represented 40-75% of total white blood cells. Cats were negative after testing for infection with Feline Leukemia Virus and Feline Immunodef...

show abstract

Section: Discussionmentioning

confidence: 95%