The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a potent stimulant of mast cells

Montemurro, P.; Nishioka, Hiroaki; Dundon, William G.; Bernard, Marina de; Giudice, Giuseppe Del; Rappuoli, Rino; Montecucco, Cesare

doi:10.1002/1521-4141(200203)32:3<671::aid-immu671>3.3.co;2-x

Cited by 70 publications

(40 citation statements)

References 17 publications

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“…Urease is one such product that has previously been shown to stimulate macrophages (16) and to induce neutrophil TEM (Brisslert et al, unpublished), and our data suggest that H. pylori urease is important also in recruiting T cells to H. pyloriinfected mucosal tissue. In vitro studies have reported that H. pylori proteins can be actively transported through gastric epithelial monolayers (31) and that H. pylori bacteria and bacterial products can disrupt epithelial barriers (45,46). Historically, H. pylori has been considered a noninvasive bacteria (30), but in recent years electron microscopy studies have demonstrated H. pylori in the cytoplasm of gastric epithelial cells (25,34) or even in the gastric lamina propria (22).…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pyloriInduces Transendothelial Migration of Activated Memory T Cells

et al. 2005

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Helicobacter pylori infection is associated with pronounced infiltration of granulocytes and lymphocytes into the gastric mucosa, resulting in active chronic gastritis that may develop into duodenal ulcer disease or gastric adenocarcinoma. Infiltrating T cells play a major role in the pathology of these diseases, but the signals involved in recruitment of T cells from blood to H. pylori-infected tissues are not well understood. We therefore examined H. pylori-induced T-cell transendothelial migration (TEM). The Transwell system, employing a monolayer of human umbilical vein endothelial cells, was used as a model to study TEM. H. pylori induced a significant T-cell migration, compared to spontaneous migration. CD4؉ and CD8 ؉ T cells migrated to the same extent in response to H. pylori, whereas there was significantly larger transmigration of memory T cells compared to naive T cells. Both H. pylori culture filtrate and urease induced migration, and the presence of the H. pylori cag pathogenicity island increased TEM. T-cell TEM was mediated by LFA-1-ICAM-1 interactions in accordance with an increased ICAM-1 expression on the endothelial cells after contact with H. pylori. Migrating T cells had increased expression of activation marker CD69 and chemokine receptors CXCR3, CCR4, and CCR9. Furthermore, T cells migrating in response to H. pylori secreted Th1 but not Th2 cytokines upon stimulation. In conclusion, our data indicate that live H. pylori and its secreted products contribute to T-cell recruitment to the gastric mucosa and that the responding T cells have an activated memory Th1 phenotype.

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Section: Discussionmentioning

confidence: 99%

Helicobacter pyloriInduces Transendothelial Migration of Activated Memory T Cells

et al. 2005

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“…Additionally, the majority of infected patients have antibodies against this antigen, and vaccination of mice with HP-NAP induces protection against a subsequent challenge with H pylori [12] . Therefore, HP-NAP is an immune modulator promoting Th1 immune responses and an important vaccine candidate antigen [17,18] . Since HP-NAP is a powerful stimulant for the production of ROS, mediating damage to DNA and enhancing cell turnover [19] , it may be a risk factor for H pylori-associated gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer

Long¹,

Luo²,

Li³

et al. 2009

WJG

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AIM: To detect and evaluate the antibodies againstHelicobacter pylori (H pylori ) neutrophil-activating protein (HP-NAP) in patients with gastric cancer and other gastroduodenal diseases. METHODS:Recombinant HP-NAP was prepared from a prokaryotic expression system in Escherichia coli . Serum positivity and level of HP-NAP-specific antibodies in sera from 43 patients with gastric cancer, 28 with chronic gastritis, 28 with peptic ulcer, and 89 healthy controls were measured by rHP-NAP-based ELISA. rHP-NAP-stimulated production of interleukin-8 (IL-8) and growth-related oncogene (GROα) cytokines in the culture supernatant of SGC7901 gastric epithelial cells was also detected. RESULTS:The serum positivity and mean absorbance value of HP-NAP-specific antibodies in the gastric c a n c e r g r o u p ( 9 7.7 % a n d 1 . 0 1 ± 0 . 2 4 ) w e r e significantly higher than those in the chronic gastritis group (85.7% and 0.89 ± 0.14, P < 0.005) and healthy control group (27.7% and 0.65 ± 0.18, P < 0.001). The sensitivity and specificity of ELISA for the detection of HP-NAP-specific antibodies were 95.5% and 91.5%, respectively. HP-NAP could slightly upregulate IL-8 production in gastric epithelial cell lines but had no effect on GROα production. CONCLUSION:Infection with virulent H pylori strains secreting HP-NAP is associated with severe gastroduodenal diseases, and HP-NAP may play a role in the development of gastric carcinoma. rHP-NAPbased ELISA can be used as a new method to detect H pylori infection. The direct effect of HP-NAP on gastric epithelial cells may be limited, but HP-NAP may contribute to inflammatory response or carcinogenesis by activating neutrophils.

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“…The high global prevalence of H. pylori infection and its certain role in the development of gastric malignancy have encouraged researchers to study different antigens of this bacterium and investigate the possibility of their application as diagnostic indicators and/or vaccines. Overall, different antigens of this bacterium, such as VacA, NAP, Fla, and urease, have been studied for the design of vaccines and diagnostic kits (22)(23)(24)(25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

Development of an arCagA Antigen-Based Assay for the Detection of Helicobacter pylori in Stool Specimens

Bonyadi

Ghaznavi‐Rad

Mosayebi

et al. 2016

Jundishapur J Microbiol

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Background: Helicobacter pylori infection can lead to the development of gastritis and peptic ulcer in humans. Two categories of diagnostic methods are generally used for the detection of H. pylori infection. Particularly, non-invasive methods are recognized as practical, feasible, and sensitive diagnostic tests for the detection of H. pylori infection. Objectives: This study was designed with the aim to develop an assay, based on cytotoxin-associated gene A (CagA) antigen through western blotting method for the detection of H. pylori in fecal samples. Methods: The antigenic region of CagA (arCagA) gene was amplified by polymerase chain reaction (PCR) method and cloned to pET32a plasmid.

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The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a potent stimulant of mast cells

Cited by 70 publications

References 17 publications

Helicobacter pyloriInduces Transendothelial Migration of Activated Memory T Cells

Helicobacter pyloriInduces Transendothelial Migration of Activated Memory T Cells

Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer

Development of an arCagA Antigen-Based Assay for the Detection of Helicobacter pylori in Stool Specimens

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