The New Report of Root Rot on Fatsia japonica Caused by Phytophthora nicotianae in China

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Fatsia japonica plays an important role as a commonly used plant in urban landscaping. From 2022 to 2023, a root rot infestation was observed that caused extensive wilting of Fatsia japonica without leaf shedding and eventual death of the plant, severely reducing the ornamental qualities of the plant as well as the vigor of its growth. Fusarium species were isolated from the roots of the affected plants, exhibiting abundant and dense yellow mycelial colonies that proliferated radially from the center of the Petri dishes. Morphological examinations revealed the presence of falciform macro- and microconidia consistent with Fusarium, as well as chlamydospores characterized by their thick walls. For further identification, the amplification and sequencing of the ITS, TEF1 alpha, and RPB2 alpha genes were performed. Finally, healthy Fatsia japonica plants were inoculated with a spore suspension of the pathogen, to confirm that the disease symptoms were compatible with naturally occurring infection. Fusarium vanettenii was identified as the causative agent of Fatsia japonica root rot. To the best of our knowledge, this is the first report of F. vanettenii causing root rot of Fatsia japonica in China.

Section: Pathogenicity Testsmentioning

confidence: 99%

First Report of Fusarium vanettenii Causing Fusarium Root Rot in Fatsia japonica in China

Xu,

Dai,

2024

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“…Phytophthora nicotianae-induced wilt disease was recently discovered on three-year-old potted cycads in Sicily, Italy [5]. In China, P. nicotianae can cause leaf rot disease of Fatsia japonica [6]. In India and Southern Asia, P. nicotianae is one of the main pathogens among Phytophthora spp., and its hosts include, besides citrus and Nicotiana tabacum, Areca catechu, black pepper, Solanum melongena L., Cocos nucifera L., Durio zibethinus L., Psidium guajava L., orchids, Catharanthus roseus, Ananas comosus, Rosa rugosa, Hevea brasiliensis, and fragrant herbs [7].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Efficient Molecular Detection of Phytophthora nicotianae Based on RPA-CRISPR/Cas12a

Zang,

Dai,

Liu

et al. 2024

Phytophthora nicotianae is a global and polyphagous pathogen with a wide host range. P. nicotianae can infect Areca catechu, Durio zibethinus L., Psidium guajava L., Hevea brasiliensis, and other tree species. The pathogen is capable of inducing butt rot and affecting aerial parts, including stems, leaves, and fruits. Compared to other Phytophthora species, P. nicotianae is more adaptable to abiotic stress. In this study, recombinase polymerase amplification (RPA) in combination with the CRISPR/Cas12a system was used for the detection of P. nicotianae, and achieved rapid and efficient detection of P. nicotianae. The assay was highly specific to P. nicotianae. All 4 tested isolates of P. nicotianae yielded positive results, whereas 30 isolates belonging to 17 other Phytophthora species, 8 fungal species, and 4 Bursaphelenchus xylophilus vermicules lacked detection. Under the conditions of 37 °C, after 20 min of RPA reaction and 25 min of Cas12a cleavage, a DNA concentration as low as 10 pg·μL−1 could be detected. In addition, it detected P. nicotianae from artificially inoculated leaves of Fatsia japonica. In this study, a novel method was established for the efficient and accurate detection of P. nicotianae based on the combination of RPA and the CRISPR/Cas12a system.

“…The genus Phytophthora consists of more than 300 oomycete species [1] that are wellknown as important soil-borne plant pathogens [2]. Phytophthora species can cause symptoms such as rot, wilting, and ulceration, resulting in significant yield losses and even plant death [3].…”

Section: Introductionmentioning

confidence: 99%

“…Note:1 CBS, Centraalbureau voor Schimmelculture Biodiversity Centre, Utrecht, The Netherlands; ATCC, American Type Culture Collection, Manassa, VA, USA, T : ex-type isolate 2. "+" and "−" represent the positive and negative results of the RPA-CRISPR/Cas12a assay, respectively.…”

mentioning

confidence: 99%

Rapid Detection of Phytophthora cambivora Using Recombinase Polymerase Amplification Combined with CRISPR/Cas12a

Zhou,

Dai,

Dai

et al. 2023

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Phytophthora cambivora is a major quarantine pathogen that devastates economically important plants across the globe. P. cambivora causes ink disease in chestnut trees and root and stem rot in various fruit trees, resulting in significant yield reductions and plant death. Given the potential dangers of P. cambivora, effective detection methods are needed for both disease management and prevention. In this study, based on the whole-genome screening of specific target genes, a combination of the recombinase polymerase amplification technique (RPA) and CRISPR/Cas12 was established to detect P. cambivora. The RPA-CRISPR/Cas12a assay was able to specifically detect 7 target isolates of P. cambivora but did not detect the following 68 non-target isolates, including 28 isolates of Phytophthora, 3 isolates of Pythium, 3 isolates of Phytopythium, 32 isolates of fungi, and 2 isolates of Bursaphelenchus. The RPA-CRISPR/Cas12a detection method was able to detect 10 pg·μL−1 of P. cambivora genomic DNA at 37 °C within a short time span (60 min). Additionally, this method can identify the presence of P. cambivora in artificially inoculated apple fruits. In summary, compared with conventional detection techniques, the RPA-CRISPR/Cas12a detection method eliminates the need for expensive instruments, long reaction times, and high amounts of raw materials and can detect P. cambivora in imported plants at entry ports, enabling instant prevention and detection.