The New Zealand obese mouse model of obesity insulin resistance and poor breeding performance: evaluation of ovarian structure and function

Radavelli-Bagatini, Simone; Blair, Amy R.; Proietto, Joseph; Spritzer, Poli Mara; Andrikopoulos, Sofianos

doi:10.1530/joe-11-0022

Cited by 43 publications

(29 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This can be related to the reduced ovulatory rate or anovulation, explaining the poor fertility rate in this group. This finding is confirmed by Radavelli-Bagatini et al study (16). It seems these results may be due to increased serum estradiol concentration which can provide an inappropriate hormonal environment for developing follicles to be ovulated in very earliest stages of folliculogenesis (22).…”

Section: Body Weight and Fertility Ratesupporting

confidence: 64%

“…All types of follicles were counted in each ovary. Then all follicles were classified as healthy (primary, preantral, antral and preovulatory) or atretic (16). All types of follicles, cysts, and corpus lutea in each ovary were counted by two persons within 9 representative sections at least 25 μm apart.…”

Section: Evaluation Of Ovarian Morphology and Follicle Countmentioning

confidence: 99%

See 1 more Smart Citation

Polycystic Ovary Morphology (PCOM) in Estradiol Valerate Treated Mouse Model

Amini¹,

Tehranian²,

Movahedin³

et al. 2016

IJWHR

View full text Add to dashboard Cite

IntroductionPolycystic ovary syndrome (PCOS) is one of the most common endocrinopathies found among 5%-10% of reproductive age women. It is a common cause of infertility due to anovulatory (1) and hyperandrogenism (2). The name of PCOS is based on ovarian morphological changes in women who suffer from menstrual disturbances and hyperandrogenism symptoms, so polycystic ovary morphology (PCOM) can be a notable feature of PCOS (3). PCOM is revealed by some ovarian morphological abnormalities such as polycystic and enlarged ovaries (4). Although many extra ovarian causes are raised in the pathophysiology of PCOS, ovarian dysfunction plays the most important role in this field (5). Although all women with PCOS are in risk of hormonal and glucose metabolism disturbances, insulin resistance, dyslipidemia, metabolic syndrome, cardiovascular disease, and endometrial hyperplasia (4), it seems that PCOS women with PCOM are in risk of elevated serum DHEA-S levels and also taking a higher daily dose of insulin in comparison to PCOS women without PCOM (6).There are many hypotheses for the etiology of PCOS (7), but the exact initiating event in the pathophysiology of the syndrome is not entirely clear and remains poorly understood. Since the study of pathophysiology or any manipulation for treatment of PCOS/PCOM in human faces some serious logistic and ethical limitations; animal modeling is a good choice to improve the understanding of the abstruse aspects of PCOS (8). Since the 1960s, many animal models such as rodents, sheep and nonhuman primates have been applied for this purpose. Using the mouse models can provide an all-purpose tool for studying and understanding the comprehensive biologic mechanisms of PCOS/PCOM. They have stable genetic backgrounds, shorter reproductive and estrous cycle and generation times, and reasonably priced. Besides, these models have shown same reproductive and metabolic conditions as human PCOS (9). Different procedures have been suggested to produce PCOS model including prenatal or pre-pubertal androgen exposure (10,11), aromatase inhibitor or estradiol administration (11-13), and transgenic models (11). AbstractObjectives: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. Polycystic ovary (PCO) is a criterion for PCOS. Several animal models of polycystic ovary morphology (PCOM) are developed in order to study PCOS aspects. The present study aimed to develop a polycystic ovarian model in mice. Materials and Methods: Thirty-four young adult female mice were divided equally into estradiol valerate (EV) treated and control groups. Mice in experimental group (n = 18) received a single dose injection of EV (IM, 4 mg/kg). Weight and fertility rate of all mice were recorded. Morphology of ovarian follicles was assessed too. Chi-square and independent t test were used for comparison between groups. P value less than 0.05 was considered as significant level. Results: After 60 days, the weight of EV-treated mice was significantly lower than control (P ...

show abstract

Section: Body Weight and Fertility Ratesupporting

confidence: 64%

Section: Evaluation Of Ovarian Morphology and Follicle Countmentioning

confidence: 99%

Polycystic Ovary Morphology (PCOM) in Estradiol Valerate Treated Mouse Model

Amini¹,

Tehranian²,

Movahedin³

et al. 2016

IJWHR

View full text Add to dashboard Cite

show abstract

“…NZO is a polygenic model of obesity, insulin resistance, leptin insensitivity, glucose intolerance, and hyperinsulinemia. [6] Fed with carbohydrate-rich diet, those animals become quickly resistant to insulin and eventually diabetic. At the same time, they develop obesity.…”

Section: Introductionmentioning

confidence: 99%

“Cyt/Nuc,” a Customizable and Documenting ImageJ Macro for Evaluation of Protein Distributions Between Cytosol and Nucleus

Grune

Kehm

Höhn

et al. 2018

Biotechnology Journal

View full text Add to dashboard Cite

Large amounts of data from multi-channel, high resolution, fluorescence microscopic images require tools that provide easy, customizable, and reproducible high-throughput analysis. The freeware "ImageJ" has become one of the standard tools for scientific image analysis. Since ImageJ offers recording of "macros," even a complex multi-step process can be easily applied fully automated to large numbers of images, saving both time and reducing human subjective evaluation. In this work, we present "Cyt/Nuc," an ImageJ macro, able to recognize and to compare the nuclear and cytosolic areas of tissue samples, in order to investigate distributions of immunostained proteins between both compartments, while it documents in detail the whole process of evaluation and pattern recognition. As practical example, the redistribution of the 20S proteasome, the main intracellular protease in mammalian cells, is investigated in NZO-mouse liver after feeding the animals different diets. A significant shift in proteasomal distribution between cytosol and nucleus in response to metabolic stress was revealed using "Cyt/ Nuc" via automatized quantification of thousands of nuclei within minutes. "Cyt/Nuc" is easy to use and highly customizable, matches the precision of careful manual evaluation and bears the potential for quick detection of any shift in intracellular protein distribution.

show abstract

“…Although they have normal leptin and leptin receptor genes, NZO mice exhibited a defect in leptin transport across the blood-brain barrier. Along with obesity, the NZO mouse exhibited insulin resistance and hyperinsulinemia, all of which are common metabolic abnormal characteristics of PCOS [99]. Furthermore, the mouse displayed dyslipidemia, hypercholesterolemia, and hypertension [100].…”

Section: Leptin Mutant Rodent Strainsmentioning

confidence: 99%

Rodent models of polycystic ovary syndrome

McNeilly¹,

Duncan²

2013

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

Polycystic ovary syndrome (PCOS) is the most frequent female endocrine disorder, affecting 5%-10% of women, causing infertility due to dysfunctional follicular maturation and ovulation, distinctive multicystic ovaries and hyperandrogenism, together with metabolic abnormalities including obesity, hyperinsulinism, an increased risk of type 2 diabetes, and cardiovascular disease. The etiology of PCOS is unclear, and decisive clinical studies are limited by ethical and logistic constraints. Consequently treatment is palliative rather than curative and focuses on symptomatic approaches. Hence, a suitable animal model could provide a valuable means with which to study the pathogenesis of the characteristic reproductive and metabolic abnormalities and thereby identify novel and more effective treatments. So far there is no consensus on the best experimental animal model, which should ideally reproduce the key features associated with human PCOS. The prenatally androgenized rhesus monkey displays many characteristics of the human condition, including hyperandrogenism, anovulation, polycystic ovaries, increased adiposity, and insulin insensitivity. However, the high cost of nonhuman primate studies limits the practical utility of these large-animal models. Rodent models, on the other hand, are inexpensive, provide well-characterized and stable genetic backgrounds readily accessible for targeted genetic manipulation, and shorter reproductive life spans and generation times. Recent rodent models display both reproductive and metabolic disturbances associated with human PCOS. This review aimed to evaluate the rodent models reported to identify the advantages and disadvantages of the distinct rodent models used to investigate this complex endocrine disorder.animal models, fertility, follicular development, ovary, PCOS

show abstract

The New Zealand obese mouse model of obesity insulin resistance and poor breeding performance: evaluation of ovarian structure and function

Cited by 43 publications

References 76 publications

Polycystic Ovary Morphology (PCOM) in Estradiol Valerate Treated Mouse Model

Polycystic Ovary Morphology (PCOM) in Estradiol Valerate Treated Mouse Model

“Cyt/Nuc,” a Customizable and Documenting ImageJ Macro for Evaluation of Protein Distributions Between Cytosol and Nucleus

Rodent models of polycystic ovary syndrome

Contact Info

Product

Resources

About