2019
DOI: 10.1093/femsyr/foz006
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The NHR1-1 of Prs1 and the pentameric motif 284KKCPK288 of Prs3 permit multi-functionality of the PRPP synthetase in Saccharomyces cerevisiae

Abstract: The five-membered PRS gene family of Saccharomyces cerevisiae is an example of gene duplication allowing the acquisition of novel functions. Each of the five Prs polypeptides is theoretically capable of synthesising PRPP but at least one of the following heterodimers is required for survival: Prs1/Prs3, Prs2/Prs5 and Prs4/Prs5. Prs3 contains a pentameric motif 284 KKCPK 288 found only in nuclear proteins. Deletion of 284 KKCPK 288 destabilises the Prs1/Prs3 complex resulting in a cascade of events, including r… Show more

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Cited by 4 publications
(22 citation statements)
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“…A strain deleted for one of the cell surface sensors, Mid2 ( Figure 4 b), thereby activating the CWI pathway, is also sensitive to α-factor, but the combination of a mid2Δ deletant with either a prs1Δ or prs3Δ deletant did not increase the α-factor sensitivity, suggesting that the Prs1 and Prs3 polypeptides interact with downstream components of this pathway [ 78 , 82 ]. Further evidence for the interaction of Prs polypeptides with the CWI pathway has been demonstrated in an extensive Y2H analysis revealing that Rlm1, an endpoint of the CWI pathway, interacts with Prs1, Prs3 and Prs5 ( Figure 4 b) [ 74 , 78 , 79 , 92 ]. Congo red, like caffeine, is a screening agent for yeast mutants with altered cell wall architecture [ 84 , 89 ].…”
Section: Prs-encoding Genes In Saccharomyces Cerevisiaementioning
confidence: 95%
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“…A strain deleted for one of the cell surface sensors, Mid2 ( Figure 4 b), thereby activating the CWI pathway, is also sensitive to α-factor, but the combination of a mid2Δ deletant with either a prs1Δ or prs3Δ deletant did not increase the α-factor sensitivity, suggesting that the Prs1 and Prs3 polypeptides interact with downstream components of this pathway [ 78 , 82 ]. Further evidence for the interaction of Prs polypeptides with the CWI pathway has been demonstrated in an extensive Y2H analysis revealing that Rlm1, an endpoint of the CWI pathway, interacts with Prs1, Prs3 and Prs5 ( Figure 4 b) [ 74 , 78 , 79 , 92 ]. Congo red, like caffeine, is a screening agent for yeast mutants with altered cell wall architecture [ 84 , 89 ].…”
Section: Prs-encoding Genes In Saccharomyces Cerevisiaementioning
confidence: 95%
“…Excellent reviews of the yeast CWI pathway and the extent of its influence on yeast physiology have been published [ 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ]. Sc PRS mutant strains have consistently shown varying degrees of sensitivity to the purine analogue, caffeine (1,3,7-tri-methylxanthine), thus compromising CWI signalling via the MAPK (mitogen-activated protein kinase) [ 64 , 74 , 79 , 82 , 92 ]. It should be noted that caffeine not only interferes with the integrity of the cell wall, but also possibly by the inhibition of mTOR (mechanistic Target Of Rapamycin) mTORC1 and mTORC2, well-known regulators of eukaryotic cellular metabolism [ 93 , 94 , 95 ].…”
Section: Prs-encoding Genes In Saccharomyces Cerevisiaementioning
confidence: 99%
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