The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation

Wang, Y.; Yuan, Zhi Min; Wang, Jinyi; Hui-xin, Xiao; Wan, Lu; Li, Lanxin; Guo, Yan; Gong, Zhizhong; Friml, Jiřı́; Zhang, Jing

doi:10.1073/pnas.2221313120

Cited by 12 publications

(5 citation statements)

References 59 publications

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“…A series of assays confirmed the ability of PIN7 and NRT2.1 to physically interact with one another when NO 3 - levels are low, thereby suppressing the PIN7-mediated acropetal efflux of auxin, thus slowing the elongation of primary roots. Together these results support a model in which NRT2.1 is capable of influencing root growth activity through interactions with the PIN7-mediated auxin transport machinery when levels of available NO 3 - are low ( Wang et al., 2023 ).…”

Section: Plant Growth and Developmentsupporting

confidence: 74%

Functional analyses of the NRT2 family of nitrate transporters in Arabidopsis

Xu,

Cheng,

Kong

et al. 2024

Front. Plant Sci.

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Nitrogen is an essential macronutrient for plant growth and development. Nitrate is the major form of nitrogen acquired by most crops and also serves as a vital signaling molecule. Nitrate is absorbed from the soil into root cells usually by the low-affinity NRT1 NO3- transporters and high-affinity NRT2 NO3- transporters, with NRT2s serving to absorb NO3- under NO3–limiting conditions. Seven NRT2 members have been identified in Arabidopsis, and they have been shown to be involved in various biological processes. In this review, we summarize the spatiotemporal expression patterns, localization, and biotic and abiotic responses of these transporters with a focus on recent advances in the current understanding of the functions of the seven AtNRT2 genes. This review offers beneficial insight into the mechanisms by which plants adapt to changing environmental conditions and provides a theoretical basis for crop research in the near future.

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Section: Plant Growth and Developmentsupporting

confidence: 74%

Functional analyses of the NRT2 family of nitrate transporters in Arabidopsis

Xu,

Cheng,

Kong

et al. 2024

Front. Plant Sci.

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“…In this study, LBS6-treated plants showed higher expression of SYM29 , explaining the plausible reason behind the better growth in LBS6-treated plants under optimum N and N-deficient conditions. Additionally, the nitrate level in the root regulates auxin transport activity by differentially regulating the expression of NRT2.1 and thus plant growth ( Wang et al., 2023 ). Nitrate is reduced to nitrite in the cytosol by nitrate reductase, which is then transported to the chloroplast and converted to ammonia by nitrite reductase (NiR) ( Orsel et al., 2002 ).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the nitrate level in the root regulates auxin transport activity by differentially regulating the expression of NRT2.1 and thus plant growth (Wang et al, 2023). Nitrate is…”

Section: Discussionmentioning

confidence: 99%

A biostimulant prepared from red seaweed Kappaphycus alvarezii induces flowering and improves the growth of Pisum sativum grown under optimum and nitrogen-limited conditions

Shukla,

Nivetha,

Nori

et al. 2024

Front. Plant Sci.

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Nitrogen (N) is one of the critical elements required by plants and is therefore one of the important limiting factors for growth and yield. To increase agricultural productivity, farmers are using excessive N fertilizers to the soil, which poses a threat to the ecosystem, as most of the applied nitrogen fertilizer is not taken up by crops, and runoff to aquatic bodies and the environment causes eutrophication, pollution, and greenhouse gas emissions. In this study, we used LBS6, a Kappaphycus alvarezii-based biostimulant as a sustainable alternative to improve the growth of plants under different NO3- fertigation. A root drench treatment of 1 ml/L LBS6 significantly improved the growth of Pisum sativum plants grown under optimum and deficient N conditions. No significant difference was observed in the growth of LBS6-treated plants grown with excessive N. The application of LBS6 induced flowering under optimum and deficient N conditions. The total nitrogen, nitrate and ammonia contents of tissues were found to be higher in treated plants grown under N deficient conditions. The LBS6 treatments had significantly higher chlorophyll content in those plants grown under N-deficient conditions. The root drench application of LBS6 also regulated photosynthetic efficiency by modulating electron and proton transport-related processes of leaves in the light-adapted state. The rate of linear electron flux, proton conductivity and steady-state proton flux across the thylakoid membrane were found to be higher in LBS6-treated plants. Additionally, LBS6 also reduced nitrogen starvation-induced, reactive oxygen species accumulation by reduction in lipid peroxidation in treated plants. Gene expression analysis showed differential regulation of expression of those genes involved in N uptake, transport, assimilation, and remobilization in LBS6-treated plants. Taken together, LBS6 improved growth of those treated plants under optimum and nitrogen-limited condition by positively modulating their biochemical, molecular, and physiological processes.

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“…NRT2.1, in contrast to NPF6.3/NRT1.1, is a unidirectional nitrate transporter, which is not required for nitrate‐induced calcium influx, and is not required for nitrate‐dependent alleviation of proton toxicity (Fang et al ., 2016). Recently, progress has been made on how NRT2.1 exerts its effects on nitrate regulation of root growth, with a direct interaction between NRT2.1 and the auxin transporter PIN7 serving to inhibit PIN7‐mediated auxin efflux, depending on nitrate availability (Wang et al ., 2023).…”

Section: Nitrogen Uptake and Regulationmentioning

confidence: 99%

Microbe‐dependent and independent nitrogen and phosphate acquisition and regulation in plants

Zhao,

Jia,

et al. 2023

New Phytologist

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SummaryNitrogen (N) and phosphorus (P) are the most important macronutrients required for plant growth and development. To cope with the limited and uneven distribution of N and P in complicated soil environments, plants have evolved intricate molecular strategies to improve nutrient acquisition that involve adaptive root development, production of root exudates, and the assistance of microbes. Recently, great advances have been made in understanding the regulation of N and P uptake and utilization and how plants balance the direct uptake of nutrients from the soil with the nutrient acquisition from beneficial microbes such as arbuscular mycorrhiza. Here, we summarize the major advances in these areas and highlight plant responses to changes in nutrient availability in the external environment through local and systemic signals.

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The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation

Cited by 12 publications

References 59 publications

Functional analyses of the NRT2 family of nitrate transporters in Arabidopsis

Functional analyses of the NRT2 family of nitrate transporters in Arabidopsis

A biostimulant prepared from red seaweed Kappaphycus alvarezii induces flowering and improves the growth of Pisum sativum grown under optimum and nitrogen-limited conditions

Microbe‐dependent and independent nitrogen and phosphate acquisition and regulation in plants

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