The nitrification inhibitor 3,4-dimethylpyrazole-phosphat (DMPP) - quantification and effects on soil metabolism

Benckiser, Gero; Christ, Elke; Herbert, T.; Weiske, A.; Blome, J.; Hardt, Martin

doi:10.1007/s11104-013-1664-6

Cited by 82 publications

(53 citation statements)

References 38 publications

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“…Our results were consistent with those from other studies using DMPP on similar soil types in this region (De Antoni Migliorati et al 2014;Scheer et al 2014Scheer et al , 2016a and from other climates and production systems (Pasda et al 2001;Chaves et al 2006;Benckiser et al 2013). All have consistently reported effective nitrification inhibition periods of 60-90 days.…”

Section: Effects Of Dmpp On N 2 O Emissions and Grain Yieldssupporting

confidence: 92%

Comparison of grain yields and N2O emissions on Oxisol and Vertisol soils in response to fertiliser N applied as urea or urea coated with the nitrification inhibitor 3,4-dimethylpyrazole phosphate

Migliorati

Bell

Lester³

et al. 2016

Soil Res.

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Abstract. The potential for elevated nitrous oxide (N 2 O) losses is high in subtropical cereal cropping systems in northeast Australia, where the fertiliser nitrogen (N) input is one single application at or before planting. The use of urea coated with the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) has been reported to substantially decrease N 2 O emissions and increase crop yields in humid, high-intensity rainfall environments. However, it is still uncertain whether this product is similarly effective in contrasting soil types in the cropping region of north-east Australia. In this study the grain yield response of sorghum (Sorghum bicolor L. Moench) to rates of fertiliser N applied as urea or urea coated with DMPP were compared in crops grown on a Vertisol and an Oxisol in southern Queensland. Seasonal N 2 O emissions were monitored on selected treatments for the duration of the cropping season and the early stages of a subsequent fallow period using a fully automated high-frequency greenhouse gas measuring system. On each soil the tested treatments included an unfertilised control (0 kg N ha -1 ) and two fertilised treatments chosen on the basis of delivering at least 90% of seasonal potential grain yield (160 and 120 kg N ha -1 on the Vertisol and Oxisol respectively) or at a common (suboptimal) rate at each site (80 kg N ha -1 ). During this study DMPP had a similar impact at both sites, clearly inhibiting nitrification for up to 8 weeks after fertiliser application. Despite the relatively dry seasonal conditions during most of the monitoring period, DMPP was effective in abating N 2 O emissions on both soils and on average reduced seasonal N 2 O emissions by 60% compared with conventional urea at fertiliser N rates equivalent to those producing 90% of site maximum grain yield. The significant abatement of N 2 O emissions observed with DMPP, however, did not translate into significant yield gains or improvements in agronomic efficiencies of fertiliser N use. These results may be due to the relatively dry growing season conditions before the bulk of crop N acquisition, which limited the exposure of fertiliser N to large losses due to leaching and denitrification.

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Section: Effects Of Dmpp On N 2 O Emissions and Grain Yieldssupporting

confidence: 92%

Comparison of grain yields and N2O emissions on Oxisol and Vertisol soils in response to fertiliser N applied as urea or urea coated with the nitrification inhibitor 3,4-dimethylpyrazole phosphate

Migliorati

Bell

Lester³

et al. 2016

Soil Res.

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“…Firstly, it is conventionally considered that hydroxylamine (NH 2 OH) acts as the intermediate of ammonia oxidation (NH 3 → NH 2 OH), catalyzed by the AMO enzyme, and DMPP could inhibit nitrification through inactivation of the AMO activity (Benckiser et al 2013). However, the homologous gene encoding the hydroxylamine oxidoreductase (HAO), which is a necessity for the oxidation of NH 2 OH, has not been identified in AOA genomes (Tourna et al 2011).…”

Section: Discussion Dmpp Inhibits Nitrification Through Impairing Thementioning

confidence: 99%

“…More importantly, no negative effect of DMPP on non-target microorganisms in soils has been reported (Tindaon et al 2012;Kong et al 2016). The inhibition of nitrification by DMPP was previously thought to be through indiscriminately binding to the complex of membrane-bound proteins and inactivating the AMOcontaining AOB (Kleineidam et al 2011;Benckiser et al 2013). This perception, however, was challenged by the recent studies of Dong et al (2013) and Florio et al (2014), which provided evidence that DMPP inhibited AOA as well.…”

Section: Introductionmentioning

confidence: 99%

Effects of 3,4-dimethylpyrazole phosphate (DMPP) on nitrification and the abundance and community composition of soil ammonia oxidizers in three land uses

Shi

et al. 2016

Biol Fertil Soils

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The application of the nitrification inhibitor, 3,4-dimethylpyrazole-phosphate (DMPP), is considered as an effective strategy to mitigate agricultural nitrogen loss. However, the inhibitory effect of DMPP on nitrification is variable and the importance of the soil microbial community composition to the variability is poorly understood. In this study, nine soils were collected across three land uses to investigate the impact of DMPP on nitrification and associated dynamics of ammonia oxidizers in a 28-day microcosm incubation. The results showed that the efficacy of DMPP at inhibiting net nitrification rates varied highly from no effect to 63.6 % during the first week of incubation. The abundance of ammonia-oxidizing bacteria (AOB), rather than ammoniaoxidizing archaea (AOA), was significantly correlated with nitrate concentrations across three land uses and significantly inhibited by DMPP addition. DMPP had higher efficacy in neutral and alkaline wheat and vegetable soils, compared with pasture soils. Canonical correspondence analysis suggested that soil pH was the most influential factor explaining the community composition of AOB and AOA in the collected soils. However, neither ammonium nitrate nor DMPP addition had a significant effect on the community composition of AOB or AOA during the incubation indicated by non-metric multidimensional scaling ordination. Taken together, our findings indicated that DMPP slowed nitrification by inhibiting the growth of AOB, and DMPP application affected the abundance of AOB more than the ammonia oxidizer community composition.

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“…Two of the most widely used NI are dicyandiamide (DCD) and dimethylpyrazole-phosphate (DMPP) (Liu et al 2013). DCD blocks the electron transport chain in the cytochrome of ammonia monoxygenase (AMO), whereas DMPP binds indiscriminately to the membrane-bound AMO (Chaves et al 2006;Fiencke and Bocke 2006;Benckiser et al 2013), delaying the first and rate-limiting step of nitrification (the oxidation of NH 4 + to NO 2 − ; Zerulla et al 2001). In comparison to DCD, DMPP has been shown to be less phytotoxic and lower application rates are required Zerulla et al 2001;Di and Cameron 2012).…”

Section: Introductionmentioning

confidence: 99%

The mobility of nitrification inhibitors under simulated ruminant urine deposition and rainfall: a comparison between DCD and DMPP

et al. 2016

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Urine patches within pasture soils are hotspots for nitrogen (N) cycling and losses, where nitrification inhibitors (NI) offer a means of reducing such losses. Within urine influenced soil, more research has been conducted for dicyandiamide (DCD) than 3,4-dimethylpyrazole phosphate (DMPP). Differences in the efficacy of these NI are often ascribed to a greater mobility of DCD, which may lead to spatial separation from NH 4 + and nitrifying microorganisms. We tested the mobility of 14 C-labelled DCD and DMPP relative to sheep urine-derived NH 4 + in soil columns of contrasting texture and organic matter content, following simulated rainfall. We also assessed factors influencing the vertical mobility of these NI in soils, including solubility, sorption/ desorption processes and microbial degradation and uptake. Following 40-mm rainfall, without the presence of sheep urine, the distribution of both NI were similar in the soil columns; however, there was a greater retention of DCD compared to DMPP in the top 1 cm. Both NI appeared to co-locate well with urine-derived NH 4 + , and the presence of sheep urine altered the leaching profile of the NI (compared to rainfall application alone), but this effect was inhibitor and soil-type dependent. A greater sorption to the soil matrix was observed for DCD in comparison to DMPP in all three studied soils, and the presence of urine generally increased desorption processes. Of the NI applied to the soil columns, 18-66 % was taken up within 30 min by the microbial community. However, only small amounts (<1 %) were mineralized during this period. In conclusion, due to the greater adsorption of DCD as opposed to DMPP and similarity in the degree of co-location of both NI with urine NH 4 + , the results of this study suggest that differences in microbial uptake and degradation may be more important parameters for explaining differences in the efficacy of reducing nitrification. Further work is required to determine the comparative efficacy of both NI in reducing nitrification rates under field conditions in a range of soil types and environmental conditions.

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The nitrification inhibitor 3,4-dimethylpyrazole-phosphat (DMPP) - quantification and effects on soil metabolism

Cited by 82 publications

References 38 publications

Comparison of grain yields and N2O emissions on Oxisol and Vertisol soils in response to fertiliser N applied as urea or urea coated with the nitrification inhibitor 3,4-dimethylpyrazole phosphate

Comparison of grain yields and N2O emissions on Oxisol and Vertisol soils in response to fertiliser N applied as urea or urea coated with the nitrification inhibitor 3,4-dimethylpyrazole phosphate

Effects of 3,4-dimethylpyrazole phosphate (DMPP) on nitrification and the abundance and community composition of soil ammonia oxidizers in three land uses

The mobility of nitrification inhibitors under simulated ruminant urine deposition and rainfall: a comparison between DCD and DMPP

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