The Nitroblue Tetrazolium Test

Gordon, Andrew M.; Rm, Rowan

doi:10.1177/003693307301800105

Cited by 10 publications

(4 citation statements)

References 30 publications

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“…Furthermore, Nishikimi, Rao & Yagi (1972) have shown that superoxide will reduce NBT in a cell-free system, using phenazine methosulphate as the intermediate electron carrier. It is essential that each laboratory employing an NBT test must establish its own range of norms for blood neutrophii activity, and the various technical factors to be considered have been reviewed (Gordon & Rowan 1973, Segal 1974, Freeman & King 1975.…”

Section: Discussionmentioning

confidence: 99%

Functional integrity of gingival crevicular neutrophil polymorphonuclear leucocytes as demonstrated by nitroblue tetrazolium reduction

Kowolik

Raeburn

1980

J of Periodontal Research

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Gingival crevicular neutrophils were collected from 20 healthy subjects and their ability to reduce nitroblue tetrazolium was investigated by means of a semi‐quantitative histochemical test. 78.04 % (± s.e.m. 0.8 %) of the cells were vital. 42.9 % (± s.e.m. 3.3 %) of the crevice neutrophils were NBT positive as compared to 5.6% (± s.e.m. 0.89%) of unstimulated peripheral venous blood neutrophils from the same patients (p < 0.001). No difference between male and female was found. The results indicate a stimulation of crevicular neutrophils by bacteria. Furthermore, a slight correlation between blood and crevicular neutrophil activity in each individual was found, suggesting that even in the normal state some antigen or other bacterial product in the circulation is capable of stimulating the neutrophils. One patient with hereditary cyclical neutropenia, shown to have a greatly reduced number of crevicular cells, had normal activity of both blood and crevicular neutrophils as measured by this test. These findings, compatible with known immunological results, are compared with the neutrophil function test results in this patient and two other neutropenic subjects. This study supports the view that the gingival crevicular neutrophil is a functional cell capable of contributing towards host defense.

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Section: Discussionmentioning

confidence: 99%

Functional integrity of gingival crevicular neutrophil polymorphonuclear leucocytes as demonstrated by nitroblue tetrazolium reduction

Kowolik

Raeburn

1980

J of Periodontal Research

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“…Leucocytes in cervical secretion are mostly neutrophiles that play an important role in protecting the reproductive tract from infection [8] and are an important component of the non-specific element of the immune system, so we studied their functional activity in detail. We studied phagocyte activity of neutrophiles in the cervical secretion due to their ability to absorb the latex microspheres and found that the patients with Chlamydia cervitis had much lower activity and intensity of phagocytes and statistically they were significantly different from the results of the intact women (p < 0,005).…”

Section: Results Coursementioning

confidence: 99%

Low intensity laser effects on factors of congenital immunity and cytokine balance in secretions of female reproductive system affected with chlamydia infection

2019

Photobiology and Photomedicine

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Therefore, we defined the objective of our research: to study the factors of uterine cervix tissue im- munity for women with Chlamydia infection and to estimate the effect of low-intensity laser therapy (LILT) on these factors due to a wavelength of 632 nm and a power of 30 mW. Materials and methods. Clinical immunologic studies of cervical secretion were carried out on 184 women of reproductive age 25,9 ± 4,22 in 2016–2018. Chlamydia infection Chlamydia trachomatis was found by the methods of real-time polymerase chain reaction (Rt-PCR) tests. Code by Classifier of the International Standard Classification of Diseases and Related Health Problems 10th Revision is А 56.0, Chlamydial infections of the lower sections of the urogenital tract. Urethra and canalis cervicis uteri scrapings were used as the material for detecting C. trachomatis. All patients with chlamydial infection of the lower part of the urogenital system were divided into two groups: a group of 100 women who were treated by using the local influence of the low-intensity laser of 632 nm as a part of complex treatment, the comparison group (basic treatment) consisted of 84 women who took azithromycin of 1 g once a day for the 1st, 5th, 10th days of treatment. 50 healthy women without any pathology and infections of the genitourinary system made the control group. A system of cytokines was studied by interleukin's level IL-8. IL-1 α, IL-1 β, TNF-α and IFN-γ content in cervical mucus. Results Course. The results of the study showed a high clinical and immunological efficacy of treating chlamydial cervicitis using a low intensity laser with a wavelength of 632 nm. An imbalance of cellular factors of innate immunity revealed before starting a therapy: a decrease in the oxygen-dependent metabolism of neutrophilic granulocytes of the cervical secretion.

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“…In contrast, the development of secondary polycythemia is mediated through accelerated erythropoietin formation which, in turn, stimulates the differentiation of bone marrow stem cells into erythropoietic precursors (9). Erythropoietin is produced from a serum glycoprotein and, to a large extent (90 %), under the influence of a renal erythropoietic factor (REF) (10). Since nephrectomy apparently reverses the plethoric condition, as mentioned in the introduction, intrarenal nickel sulfide administration presumably stimulates the REF and, consequently, erythropoietin production, thereby eliciting polycythemia.…”

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confidence: 99%