The Nitrogen Terminal End-Groups of Hypophyseal Growth Hormone

Spectrophotometric Study of the Reaction of Protein Sulfhydryl Groups with Organic Mercurials

“…Protein Preparations.-The growth hormone preparations were isolated from ox anterior pituitary glands by the methods previously described19-21; they had been shown to behave as homogeneous substances according to electrophoretic, sedimentation and diffusion studies19'21 and have recently been found to be essentially homogeneous by zone electrophoresis on starch22 and end group analysis. 23 Growthpromoting activity of the hormone was estimated by the tibia test in hypophysectomized rats. 24 The ACTH fraction employed was a peptic digest of a concentrate prepared from sheep glands, obtained by procedures previously described.…”

Adsorption Chromatography of Hypophyseal Growth Hormone and Other Proteins on Hyflo Super-cel Columns

“…Protein Preparations.-The growth hormone preparations were isolated from ox anterior pituitary glands by the methods previously described19-21; they had been shown to behave as homogeneous substances according to electrophoretic, sedimentation and diffusion studies19'21 and have recently been found to be essentially homogeneous by zone electrophoresis on starch22 and end group analysis. 23 Growthpromoting activity of the hormone was estimated by the tibia test in hypophysectomized rats. 24 The ACTH fraction employed was a peptic digest of a concentrate prepared from sheep glands, obtained by procedures previously described.…”

Biochemical Analysis

“…As has been true almost from the inception of paper chromatography, major interest still continues in amino acid and polypeptide separation, identification, and determination from the standpoint of method (40,169,170,172,173,177,213,296,369,391,401,403,429,512) and for numerous specific purposes, including analysis of proteins (168,281), resolution of the optical isomers of tyrosine and glutamic acid (432), determination of peptide end groups (235) and terminal amino acids of bovine plasma albumin (186), of ovomucoid (389), and of hypophysial growth hormone (300), clinical studies of amino acid metabolism (313,330), and comparative studies of the blood amino acids of mammals (129), insects (15), and silkworms (158). The specific problems of chromatography of protein split products as it applies to amino acid metabolism were reviewed by Awapara (17), and a more general comparative review of this technique in comparison with other types of fractionation procedures was published by Boulanger and Biserte (45).…”