The noa gene is functionally linked to the activation of the Toll/Imd signaling pathways in Bactrocera dorsalis (Hendel)

Dong, Xinxin; Li, Qiujia; Zhang, Hongyu

doi:10.1016/j.dci.2015.09.009

Cited by 21 publications

(19 citation statements)

References 29 publications

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“…The glass capillaries used for microinjection were made from 50 µl glass micropipettes using a Puller at heater level 60.4 (PC-10, Narishige, Japan). The injection conditions were set to a Pi of 570 hpa and a Ti of 0.2 s. A total of 200 nl of solution was injected into each B. dorsalis fly 34 .…”

Section: Methodsmentioning

confidence: 99%

The inducible blockage of RNAi reveals a role for polyunsaturated fatty acids in the regulation of dsRNA-endocytic capacity in Bactrocera dorsalis

Dong

et al. 2017

Sci Rep

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Exogenous double-stranded RNA (dsRNA) can trigger gene silencing through the RNA interference (RNAi) pathway. Our previous research established that Bactrocera dorsalis can block RNAi after an initial priming of exposure to dsRNA. However, the mechanism underlying this phenomenon is not yet fully understood. Here, we demonstrate that fatty acid biosynthesis and metabolism pathways play important roles in the blockage of RNAi induced by dsRNA priming. The ratio of linoleic acid (LA) to arachidonic acid (AA) was significantly increased in the hemolymph of B. dorsalis following dsRNA priming, and further, the endocytosis of dsRNA into the midgut cells of B. dorsalis was inhibited in these samples. The expression levels of most genes involved in the fatty acid biosynthesis and metabolism pathways were altered following priming with dsRNA. Furthermore, altering the composition of fatty acids via the injection of AA can facilitate the uptake of ingested dsRNA into the midgut cells of Drosophila melanogaster and successfully induce an RNAi effect, which cannot be achieved via feeding in fruit flies. Our results suggest that polyunsaturated fatty acids are involved in the regulation of the dsRNA-endocytic ability in B. dorsalis.

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Section: Methodsmentioning

confidence: 99%

The inducible blockage of RNAi reveals a role for polyunsaturated fatty acids in the regulation of dsRNA-endocytic capacity in Bactrocera dorsalis

Dong

et al. 2017

Sci Rep

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“…Plasmids were transformed into Escherichia coli HT115 (DE3) competent cells and individual colonies were cultured at 37 °C in 500 ml of LB medium and induced to generate dsRNA with 0.4 mM isopropyl-D-thiogalactoside (IPTG). dsRNA was extracted from aliquots of bacteria with a recently described protocol3940.…”

Section: Methodsmentioning

confidence: 99%

Knockdown of the MAPK p38 pathway increases the susceptibility of Chilo suppressalis larvae to Bacillus thuringiensis Cry1Ca toxin

Qiu

Fan

Zhang

et al. 2017

Sci Rep

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The bacterium Bacillus thuringiensis (Bt) produces a wide range of toxins that are effective against a number of insect pests. Identifying the mechanisms responsible for resistance to Bt toxin will improve both our ability to control important insect pests and our understanding of bacterial toxicology. In this study, we investigated the role of MAPK pathways in resistance against Cry1Ca toxin in Chilo suppressalis, an important lepidopteran pest of rice crops. We first cloned the full-length of C. suppressalis mitogen-activated protein kinase (MAPK) p38, ERK1, and ERK2, and a partial sequence of JNK (hereafter Csp38, CsERK1, CsERK2 and CsJNK). We could then measure the up-regulation of these MAPK genes in larvae at different times after ingestion of Cry1Ca toxin. Using RNA interference to knockdown Csp38, CsJNK, CsERK1 and CsERK2 showed that only knockdown of Csp38 significantly increased the mortality of larvae to Cry1Ca toxin ingested in either an artificial diet, or after feeding on transgenic rice expressed Cry1Ca. These results suggest that MAPK p38 is responsible for the resistance of C. suppressalis larvae to Bt Cry1Ca toxin.

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“…Positive clones were cultured at 378C in 100 ml Lysogeny Broth (LB) medium containing 50 lg/ml kanamycin and induced to generate dsRNA by the addition of 0.4 mM isopropyl-Dthiogalactoside, after which the bacteria were cultured for an additional 4 h at 378C. dsRNA was extracted according to the method described by Timmons et al (2001) and Dong et al (2016).…”

Section: Preparation Of Bacterially Expressed Dsrnamentioning

confidence: 99%

Downregulation of Chilo suppressalis alkaline phosphatase genes associated with resistance to three transgenic Bacillus thuringiensis rice lines

Qiu

Wang

et al. 2017

Insect Molecular Biology

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Insecticidal crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis are highly toxic to lepidopteran pests. Strains of transgenic rice expressing cry genes have been developed that are resistant to rice pests. Understanding the mode of action of Cry toxins in rice pests will improve our ability to use them effectively as insecticides. In this study, we tested the hypothesis that alkaline phosphatases (ALPs) are involved in Cry1A, Cry2Aa and Cry1Ca toxicity in Chilo suppressalis, an important insect pest of rice crops in China. We first cloned three novel C. suppressalis alps (Csalps) from the larval midgut of C. suppressalis. RNA interference knockdown of six different Csalp genes (Csalp1, Csalp2, Csalp3, Csalp4, Csalp5 and Csalp6) showed that knockdown of three of these, Csalp1, Csalp2 and Csalp4, reduced larval mortality to the transgenic rice strain TT51, which expresses a fusion protein of Cry1Ab and Cry1Ac, whereas suppression of Csalp1, Csalp2, Csalp3, Csalp4 and Csalp6 transcripts decreased the susceptibility of larvae to the transgenic rice strain T2A-1, which expresses cry2Aa.Moreover, downregulation of Csalp1, Csalp2, Csalp3, Csalp4 and Csalp5 transcripts conferred significant tolerance to the transgenic rice strain T1C-19, which expresses cry1Ca. These results suggest that these ALPs play a key role in the toxicity of Cry1A, Cry2A and Cry1C to C. suppressalis.

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The noa gene is functionally linked to the activation of the Toll/Imd signaling pathways in Bactrocera dorsalis (Hendel)

Cited by 21 publications

References 29 publications

The inducible blockage of RNAi reveals a role for polyunsaturated fatty acids in the regulation of dsRNA-endocytic capacity in Bactrocera dorsalis

The inducible blockage of RNAi reveals a role for polyunsaturated fatty acids in the regulation of dsRNA-endocytic capacity in Bactrocera dorsalis

Knockdown of the MAPK p38 pathway increases the susceptibility of Chilo suppressalis larvae to Bacillus thuringiensis Cry1Ca toxin

Downregulation of Chilo suppressalis alkaline phosphatase genes associated with resistance to three transgenic Bacillus thuringiensis rice lines

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