The NOBOX protein becomes undetectable in developmentally competent antral and ovulated oocytes

Belli, Martina; Cimadomo, Danilo; Merico, Valeria; Redi, Carlo Alberto; Garagna, Silvia; Zuccotti, Maurizio

doi:10.1387/ijdb.120125mz

Cited by 19 publications

(21 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To examine whether NLBs can also contain nuclear proteins, we immunolabeled proteinase K-treated oocytes with antibodies to the nuclear splicing factor SC35, NOBOX (essential for folliculogenesis and regulation of oocyte-specific genes; Belli et al, 2013), topoisomerase II beta (involved in RNA transcription and DNA replication; Chen et al, 2013), heterochromatin protein HP1α (involved in regulation of gene transcription; Canzio et al, 2014), and the core nucleohistone H3 (Table 1). All these proteins except for SC35 and NOBOX have been reported in the proteome of mouse somatic nucleoli (Kar et al, 2011).…”

Section: Immunolabeling Of Oocytes With Antibodies To Key Nucleolar Amentioning

confidence: 99%

Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA

Shishova

Lavrentyeva

Dobrucki

et al. 2015

Developmental Biology

View full text Add to dashboard Cite

It is well known that fully-grown mammalian oocytes, rather than typical nucleoli, contain prominent but structurally homogenous bodies called "nucleolus-like bodies" (NLBs). NLBs accumulate a vast amount of material, but their biochemical composition and functions remain uncertain. To clarify the composition of the NLB material in mouse GV oocytes, we devised an assay to detect internal oocyte proteins with fluorescein-5-isothiocyanate (FITC) and applied the fluorescent RNA-binding dye acridine orange to examine whether NLBs contain RNA. Our results unequivocally show that, similarly to typical nucleoli, proteins and RNA are major constituents of transcriptionally active (or non-surrounded) NLBs as well as of transcriptionally silent (or surrounded) NLBs. We also show, by exposing fixed oocytes to a mild proteinase K treatment, that the NLB mass in oocytes of both types contains nucleolar proteins that are involved in all major steps of ribosome biogenesis, including rDNA transcription (UBF), early rRNA processing (fibrillarin), and late rRNA processing (NPM1/nucleophosmin/B23, nucleolin/C23), but none of the nuclear proteins tested, including SC35, NOBOX, topoisomerase II beta, HP1α, and H3. The ribosomal RPL26 protein was detected within the NLBs of NSN-type oocytes but is virtually absent from NLBs of SN-type oocytes. Taking into account that the major class of nucleolar RNA is ribosomal RNA (rRNA), we applied fluorescence in situ hybridization with oligonucleotide probes targeting 18S and 28S rRNAs. The results show that, in contrast to active nucleoli, NLBs of fully-grown oocytes are impoverished for the rRNAs, which is consistent with the absence of transcribed ribosomal genes in the NLB mass. Overall, the results of this study suggest that NLBs of fully-grown mammalian oocytes serve for storing major nucleolar proteins but not rRNA.

show abstract

Section: Immunolabeling Of Oocytes With Antibodies To Key Nucleolar Amentioning

confidence: 99%

Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA

Shishova

Lavrentyeva

Dobrucki

et al. 2015

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…Among these genes are the NOBOX , which has been found to play important roles in oogenesis and folliculogenesis. [1227] In this work, we studied the prevalence of seven SNPs in the NOBOX gene and found that both cases (POR patients) and control groups represented the wild-type allele in all investigated SNPs; therefore, we exclude the role of NOBOX in POR in the studied Jordanian cohort.…”

Section: Discussionmentioning

confidence: 99%

Genetics of female infertility: Molecular study of newborn ovary homeobox gene in poor ovarian responders

et al. 2019

View full text Add to dashboard Cite

Background: Newborn ovary homeobox ( NOBOX ) gene plays a critical role in the transcriptional regulation of oocyte-specific genes. Previous studies have demonstrated a pathogenic effect of NOBOX variants on premature ovarian insufficiency (POI) patients. Poor ovarian response (POR) is a risk factor for POI. Therefore, genetic variants in the NOBOX gene may also be studied as risk factors for POR development. Aims: The aim of the study is to investigate the association between seven known NOBOX single-nucleotide polymorphisms (SNPs) and POR in Jordanian females. Settings and Design: This was a case–control study of 60 females with POR for controlled ovarian hyperstimulation and 59 healthy females with no history of reproductive problems. Blood samples were collected from the participants and seven SNPs of NOBOX gene were screened. Subjects and Methods: DNA was extracted from blood samples. Polymerase chain reaction with primers specific for seven known SNPs in NOBOX gene was used to amplify the specified region within the gene followed by Sanger sequencing. Results: The seven SNPs investigated in this study, namely, rs77587352 (c.271G>T, p. Gly91Trp), rs7800847 (c.349C>T, p. Arg117Trp), rs193303102 (c.907C>T, p. Arg303X), rs193303103 (c.1025G>C, p. Ser342Thr), rs193303104 (c.1048G>T, p. Val350Leu), rs201947677 (c.1064G>A, p. Arg355His), and rs146227301 (c.1856C>T, p. Pro619Leu), only represent the wild-type allele in both females with POR and healthy participants. Conclusions: The results show that only monomorphic genotype of the NOBOX variants was found in Jordanian females studied.

show abstract

“…To isolate ovulated MII oocytes, control and exposed females were injected intraperitoneally with 3.75 IU of pregnant mare serum gonadotropin (Folligon; Intervet, Italy) followed, 48 hr later, by an injection with 3.75 IU of human chorionic gonadotropin (Corulon; Intervet, Italy). Ovulated MII oocytes were isolated from the oviducts 15 hr after Chorulon injection according to the methodology described by Belli et al () under a stereomicroscope. The oocytes were then deprived of cumulus cells enzymatically using M2 medium containing 500 IU/ml hyaluronidase type II (Sigma‐Aldrich, Milan, Italy), and mechanically.…”

Section: Methodsmentioning

confidence: 99%

Whole‐body exposure to cigarette smoke alters oocyte miRNAs expression in C57BL/6 mice

Budani

D’Aurora

Stuppia

et al. 2019

Molecular Reproduction Devel

View full text Add to dashboard Cite

Cigarette smoke is toxic for the female reproductive system with particular reference to the ovary. The purpose of the study was to investigate if the microRNAs (miRNAs) pattern could be altered by cigarette smoke exposure in mouse oocytes. For this purpose, C57BL/6 mice were whole‐body exposed to three cigarettes daily, 7 days/week, for 2 or 4 months by a specific rodent ventilator. Mice were then superovulated and oocytes collected. MII oocytes pools obtained by single animals were deprived of cumulus cells and used to extract total RNA including miRNAs. TaqMan™ Rodent MicroRNA A Array v2.0 was used to analyze the miRNAs expression profile. The biological functions and the functional networks of the identified up‐ and downregulated miRNAs were analyzed by ingenuity pathway analysis software. The gene expression of deregulated‐miRNAs targets was evaluated. For the first time, the global miRNAs changes in mouse oocyte in response to cigarette smoke exposure were disclosed. Our results revealed significant modulation of miRNAs mainly involved in inflammatory processes, cellular proliferation, and apoptosis. miRNAs expression was altered in a time‐dependent manner. Smoke exposure induced an early downregulation of Dicer1. Transcriptional alterations of the modulated miRNAs major targets, estrogen receptor 1, peroxisome proliferator‐activated receptor‐alpha, and tumor protein 53, as well as that of other key regulatory genes, were evidenced. Cigarette smoke represents a stimulus able to alter miRNAs pattern in mouse oocyte. This study increases our understanding of the ovarian toxicity profile of cigarette smoke, and open new roads toward the identification of biomarkers of oocyte toxicity and dysregulation.

show abstract

The NOBOX protein becomes undetectable in developmentally competent antral and ovulated oocytes

Cited by 19 publications

References 16 publications

Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA

Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA

Genetics of female infertility: Molecular study of newborn ovary homeobox gene in poor ovarian responders

Whole‐body exposure to cigarette smoke alters oocyte miRNAs expression in C57BL/6 mice

Contact Info

Product

Resources

About