1994
DOI: 10.1042/bj3030171
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The non-phospholipase A2 subunit of β-bungarotoxin plays an important role in the phospholipase A2-independent neurotoxic effect: characterization of three isotoxins with a common phospholipase A2 subunit

Abstract: Three isotoxins (SP I-III) of the beta-bungarotoxin family were purified to homogeneity via a series of isolation procedures including a final step of h.p.l.c. on an SP column washed with a linear gradient of 0.2-0.6 M sodium acetate at pH 7.4. Their proportions varied greatly with the batch of venom. Each isotoxin was demonstrated by SDS/PAGE to contain a phospholipase A2 subunit and a non-phospholipase A2 subunit. The three proteins were reductively alkylated with 4-vinylpyridine and the alkylated derivative… Show more

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Cited by 29 publications
(11 citation statements)
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“…The PLA 2 superfamily has broadly been classified into four major groups; the snake venom PLA 2 s, with molecular weight ranging from 10 to 15 kDa, are classified under Groups I and II of the secretory PLA 2 (sPLA 2 ) [69]. PLA 2 enzymes display diverse pharmacological effects like neurotoxicity, cardiotoxicity, myotoxicity, necrosis, anticoagulant, hypotensive, hemolysis, hemorrhage, edema and membrane damage by several different mechanisms and PLA 2 s are therefore considered important toxic enzymes of snake venoms [28][29][30]70,71].…”
Section: Enzymatic Proteins In Pakistan Rvvmentioning
confidence: 99%
“…The PLA 2 superfamily has broadly been classified into four major groups; the snake venom PLA 2 s, with molecular weight ranging from 10 to 15 kDa, are classified under Groups I and II of the secretory PLA 2 (sPLA 2 ) [69]. PLA 2 enzymes display diverse pharmacological effects like neurotoxicity, cardiotoxicity, myotoxicity, necrosis, anticoagulant, hypotensive, hemolysis, hemorrhage, edema and membrane damage by several different mechanisms and PLA 2 s are therefore considered important toxic enzymes of snake venoms [28][29][30]70,71].…”
Section: Enzymatic Proteins In Pakistan Rvvmentioning
confidence: 99%
“…This provides a remarkable example of evolution-driven reshaping of the same scaffold to radically change its binding specificity (324,593,611). Different ␤-bungarotoxins have been isolated so far with a similar PLA 2 subunit and different binding subunits (113). Their toxicity ranges from 19 to 130 mg/kg and does not appear to correlate with the level of PLA 2 activity (311,508).…”
Section: B Structure and Enzymic Propertiesmentioning
confidence: 99%
“…So far, as many as 16 isoforms have been reported from Bungarus species [5], and 8 of them, namely b1-b5 [6] and SPI-SPIII [7], have been studied in detail. Structurally, they are covalently linked heterodimers (Fig.…”
Section: Introductionmentioning
confidence: 99%