The novel anti-hyperglycemic effect of Paeoniae radix via the transcriptional suppression of phosphoenopyruvate carboxykinase (PEPCK)

Juan, Yi‐Chen; Tsai, Wei‐Jern; Lin, Yun; Wang, G.-J.; Cheng, Jing-Jy; Yang, Haihua; Hsu, Chin-Hsien; Liu, H.-K.

doi:10.1016/j.phymed.2009.12.007

Cited by 33 publications

(34 citation statements)

References 28 publications

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“…7) Today, it has been reported that paeoniflorin exhibits many pharmacological effects such as anti-inflammation, anti-allergy, anti-hyperglycemia, analgesia and enhancing cognition. [7][8][9][10] The aim of the present study was to investigate whether paeoniflorin exerted a protective effect against on NAFLD and to determine its mechanism.…”

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confidence: 99%

Paeoniflorin Protects against Nonalcoholic Fatty Liver Disease Induced by a High-Fat Diet in Mice

Zhang

Yang

2015

Biological & Pharmaceutical Bulletin

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Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide. Paeoniflorin, a natural product and active ingredient of Paeonia lactiflora, has been demonstrated to have many pharmacological effects including antiinflammatory and antihyperglycemic activity. We investigated the effects of paeoniflorin on NAFLD in mice and its underlying mechanisms. We examined this hypothesis using a well-established animal model of NAFLD. The effects of paeoniflorin on inflammation and glucolipid metabolism disorder were evaluated. The corresponding signaling pathways were measured using real-time polymerase chain reaction (PCR). We demonstrated that the mice developed obesity, dyslipidemia, and fatty liver, which formed the NAFLD model. Paeoniflorin attenuated NAFLD and exhibited potential cardiovascular protective effects in vivo by lowering body weight, hyperlipidemia, and insulin resistance; blocking inflammation; and inhibiting lipid ectopic deposition. Further investigation revealed that the antagonistic effect on hyperlipidemia and lipid ectopic deposition was related to lowering the lipid synthesis pathway (de novo pathway, 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoAR)), promoting fatty acid oxidation [peroxisome proliferator-activated receptor-alpha (PPARα), carnitine palmitoyltransferase-1, etc.] and increasing cholesterol output (PPARγ-liver X receptor-α-ATP-binding cassette transporter-1); the inhibitory effects on inflammation and hyperglycemia were mediated by blocking inflammatory genes activation and reducing gluconeogenic genes expression (phosphoenolpyruvate carboxykinase and G6Pase). These results suggest that paeoniflorin prevents the development of NAFLD and reduces the risks of atherosclerosis through multiple intracellular signaling pathways. It may therefore be a potential therapeutic compound for NAFLD.

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confidence: 99%

Paeoniflorin Protects against Nonalcoholic Fatty Liver Disease Induced by a High-Fat Diet in Mice

Zhang

Yang

2015

Biological & Pharmaceutical Bulletin

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“…Suppression of PEPCK mRNA and protein expression by the Paeoniae Rubra Radix extract (PR-Et) has been successfully demonstrated in rats with insulin-deficient streptozotocin-induced diabetes or insulin-resistant db/db mice (Juan et al, 2010). This implied the existence of insulin mimetic actions of the PR-Et with properties that could bypass the deficit in insulin signaling found in these animal models.…”

Section: Discussionmentioning

confidence: 99%

“…Lin (Juan et al, 2010). Briefly, slices of the dried commercially available Paeoniae Rubra Radix (Paeonia lactiflora roots, 2 kg) were extracted with 80% ethanol/H 2 O at 60 • C overnight.…”

Section: Preparation Of the Npf From Paeoniae Rubra Radixmentioning

confidence: 99%

“…For signal transduction analysis, cells were harvested for protein analysis after treated with insulin (10 nM) or PGG for 30 min. H4-pPCK-GFP cells containing the PEPCK promoter regulating a green fluorescent protein (GFP) reporter were cultured with DMEM consisting of 5% FBS and G418 (2.2 mg/ml) according to previous description (Juan et al, 2010). After overnight serum starvation, cells were treated with inhibitors for 30 min prior to adding insulin, PGG or NPF.…”

Section: Cell Culturementioning

confidence: 99%

“…In addition, the suppressive effect of the PR-Et on the dexamethasone (Dex) and 8-bromo-cAMP (CA) induced PEPCK (m)RNA level appeared to be unaffected by an insulin-desensitized condition. The possibility that two major compounds, paeonol and paeoniflorin, suppressed PEPCK was ruled out (Juan et al, 2010). Therefore, both responsible bioactive component(s) and potential mechanisms of actions on PEPCK suppression by this herb remained to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Pharmacological evaluation of insulin mimetic novel suppressors of PEPCK gene transcription from Paeoniae Rubra Radix

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The novel anti-hyperglycemic effect of Paeoniae radix via the transcriptional suppression of phosphoenopyruvate carboxykinase (PEPCK)

Cited by 33 publications

References 28 publications

Paeoniflorin Protects against Nonalcoholic Fatty Liver Disease Induced by a High-Fat Diet in Mice

Paeoniflorin Protects against Nonalcoholic Fatty Liver Disease Induced by a High-Fat Diet in Mice

Pharmacological evaluation of insulin mimetic novel suppressors of PEPCK gene transcription from Paeoniae Rubra Radix

Selective suppression of microglial activation by paeoniflorin attenuates morphine tolerance

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