The novel avirulence effector AlAvr1 from <i>Ascochyta lentis</i> mediates host cultivar specificity of ascochyta blight in lentil

Henares, Bernadette M.; Debler, Johannes Wolfram; Farfan-Caceres, Lina M; Grime, C. R.; Syme, Robert A.; Blake, Sara N.; Herdina,; Davidson, J. A.; Oliver, Richard P.; Singh, Karam B.; Kamphuis, Lars G.; Lee, Robert C.

doi:10.1111/mpp.13203

Cited by 6 publications

(1 citation statement)

References 69 publications

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“…It has been reported that the nuclear effector ArPEC25 of A. rabiei manipulated the lignin biosynthesis of chickpea for invasion by targeting the transcription factor CaβLIM1a ( 37 ). The effector AlAvr1 from A. lentis mediated the host cultivar specificity of ascochyta blight of lentil ( 38 ). Homolog analysis found that the ArPEC25 homologs were highly conserved, with over 80% amino acid identities in all available genomes of Ascochyta spp.…”

Section: Discussionmentioning

confidence: 99%

Genome and Transcriptome Analysis of Ascochyta pisi Provides Insights into the Pathogenesis of Ascochyta Blight of Pea

Liu

Song

et al. 2023

Microbiol Spectr

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Section: Discussionmentioning

confidence: 99%

Genome and Transcriptome Analysis of Ascochyta pisi Provides Insights into the Pathogenesis of Ascochyta Blight of Pea

Liu

Song

et al. 2023

Microbiol Spectr

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The History and Pedigree of Australian Lentil Cultivars

Thackwray,

Materne,

Shunmugam

et al. 2024

Legume Science

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Lentils are an ancient, edible grain legume, consumed worldwide in an array of dishes as either whole or split seed. While India and Canada are the largest modern‐day producers, Australia is a close third and the second largest exporter of lentil globally. An overview of lentil introduction cultivar development and production in Australia since the 1960s is presented. This commenced with obtaining international germplasm, and in the 1970s, Australia participated in the ICARDA‐led Food Legume Improvement Program (FLIP), which saw the release of nine varieties in the span of a decade. The first local breeding efforts in Australia commenced in the 1990s through the Coordinated Improvement Program for Australian Lentils (CIPAL), which transitioned into Pulse Breeding Australia (PBA) in the 2000s, and saw the first Australian‐bred varieties released in 2008. Currently, Agriculture Victoria's National Lentil Breeding Program and Grains Innovation Australia (GIA) breed and release varieties for Australian lentil growers, and future perspectives for their programmes are presented. One of the main diseases of lentil is Ascochyta blight, which is caused by the fungus Ascochyta lentis. The discovery of a major avirulence gene within Australian A. lentis populations which determines pathotype has allowed recent categorisation of a collection of isolates, and their response to Australian varieties is discussed. The narrowing gene pool and viability of interspecific hybridisation of Australian lentil is additionally explained. Taken together, this review summarises the history and pedigree of Australian varieties and lentil breeding, the impact of major disease pathotypes on cultivar utility and the pursuits of public and private lentil breeding initiatives.

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Virulence Profiles and Genome-Wide Association Study for Ascochyta lentis Isolates Collected from Australian Lentil-Growing Regions

et al. 2023

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Ascochyta lentis, the causal organism of ascochyta blight of lentil (Lens culinaris L.), has been shown to produce an avirulence effector protein that mediates ascochyta blight (AB) resistance in certain lentil cultivars. The two known forms of the effector protein were identified from a biparental mapping population between isolates that have reciprocal virulence on PBA Hurricane XT and Nipper. The effector AlAvr1–1 was described for the PBA Hurricane XT–avirulent isolate P94–24 and AlAvr1–2 characterised in the PBA Hurricane XT-virulent isolate AlKewell. Here, we performed a genome-wide association study (GWAS) to identify other loci associated with AB for a differential set of lentil cultivars from a diverse panel of isolates collected in the Australian lentil growing regions from 2013 to 2020. The chromosome 3 AlAvr1 locus was strongly associated with the PBA Hurricane XT, Indianhead and Nipper disease responses but one other genomic region on chromosome 11 was also associated with the Nipper disease trait. Our results corroborate the earlier work that identified the AlAvr1 locus, for field-collected isolates that span the period prior to release and after widespread adoption of PBA Hurricane XT. A multiplex PCR assay was developed to differentiate the respective genes AlAvr1–1 and AlAvr1–2, to predict PBA Hurricane XT avirulence and pathotype designation in the diversity panel. Increasing numbers of the PBA Hurricane XT–virulent pathotype 2 isolates across that time indicates strong selection for isolates with the AlAvr1–2 allele. Furthermore, one other region of the A. lentis genome may contribute to the pathogen-host interaction for lentil AB.

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The novel avirulence effector AlAvr1 from Ascochyta lentis mediates host cultivar specificity of ascochyta blight in lentil

Cited by 6 publications

References 69 publications

Genome and Transcriptome Analysis of Ascochyta pisi Provides Insights into the Pathogenesis of Ascochyta Blight of Pea

Genome and Transcriptome Analysis of Ascochyta pisi Provides Insights into the Pathogenesis of Ascochyta Blight of Pea

The History and Pedigree of Australian Lentil Cultivars

Virulence Profiles and Genome-Wide Association Study for Ascochyta lentis Isolates Collected from Australian Lentil-Growing Regions

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