The Novel Function of Unsymmetrical Chiral CCN Pincer Nickel Complexes as Chemotherapeutic Agents Targeting Prostate Cancer Cells

Qu, Jingjing; Shi, Linlin; Wang, Yanbing; Yan, Jing; Shao, Tian; Hao, Xin‐Qi; Wang, Jiaxiang; Zhang, Hongyu; Gong, Jun‐Fang; Song, Bing

doi:10.3390/molecules27103106

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…Figure B demonstrates that when HeLa cells are subjected to nickel complex 4 , the proportion of early apoptotic cells is only about 1.82%, whereas the proportion of late apoptotic cells is approximately 16.7%. The cell population in the Q2 quadrant [annexin V(+)/PI(+)] was higher (16.7%) than the control (Figure A), demonstrating the induction of late apoptosis …”

Section: Resultsmentioning

confidence: 94%

“…The cell population in the Q2 quadrant [annexin V(+)/PI(+)] was higher (16.7%) than the control (Figure 8A), demonstrating the induction of late apoptosis. 36 Assessment of Caspase-3/8/9 Activation. The apoptotic mode of cancer cell death was further substantiated by caspase activation by ELISA analysis.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The cell population in the Q2 quadrant [annexin V(+)/PI(+)] was higher (16.7%) than the control ( Figure 8 A), demonstrating the induction of late apoptosis. 36 …”

Section: Resultsmentioning

confidence: 99%

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Exploration of Antiproliferative Activity and Apoptosis Induction of New Nickel(II) Complexes Encompassing Carbazole Ligands

et al. 2023

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To attest the effectiveness of nickel complexes as anticancer drug candidates with minimum side effects, the present investigation describes the facile synthesis and anticancer activities of nickel(II) complexes enriched with three derivatives of carbazolone-based benzhydrazone ligands(L) having a [Ni(L) 2 ] composition. Analytical and spectral techniques were used to characterize the synthesized Ni(II) complexes. The single-crystal X-ray diffraction performed for complex 4 confirmed the square planar geometry with a [Ni(κ 2 -N,O-L) 2 ] arrangement. The MTT assay was carried out for the complexes to determine in vitro cytotoxicity against cancerous human-cervical carcinoma, humancolon carcinoma, and non-cancerous L929 (fibroblast) cells. All three complexes exhibited good toxicity against the cancer cells with a low IC 50 concentration. Complex 4, containing −OCH 3 fragment, exhibits high lipophilicity and revealed exceptional cytotoxicity against cancer cells. AO-EB fluorescent staining indicated apoptosis-associated cell morphological changes after exposure to complex 4. The apoptosis induction was further confirmed by a HOECHST-33342 fluorescent staining technique via chromosomal condensation and nuclear fragmentation. Further, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) mechanistic studies revealed that complex 4 can raise ROS levels and reduce MMP and promote mitochondrial dysfunction-mediated apoptotic cell death. Further, stimulation of late apoptosis by complex 4 in cervical cancer cells was quantitatively differentiated through the staining of phosphatidylserine externalization by flow cytometry. Furthermore, the ELISA analysis confirmed that complex 4 induced apoptosis through caspase activation.

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Section: Resultsmentioning

confidence: 94%

Section: ■ Results and Discussionmentioning

confidence: 99%