The novel lactoferrin and DHA-codelivered liposomes with different membrane structures: Fabrication, in vitro infant digestion, and suckling pig intestinal organoid absorption
“…The degree of protein hydrolysis during digestion was measured by SDS-PAGE. 33 Digesta in stomach and small intestine collected at 30, 60, 120 and 180 min of gavage were mixed with 1 mL petroleum ether and vortexed for 20 s to extract the fat and reduce its interference with the protein assay. The subnatant was obtained after centrifugation at 16 000 g for 1 min, and the procedure was repeated three times.…”
“…The degree of protein hydrolysis during digestion was measured by SDS-PAGE. 33 Digesta in stomach and small intestine collected at 30, 60, 120 and 180 min of gavage were mixed with 1 mL petroleum ether and vortexed for 20 s to extract the fat and reduce its interference with the protein assay. The subnatant was obtained after centrifugation at 16 000 g for 1 min, and the procedure was repeated three times.…”
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