The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

Abstract

Despite the long history of breeding potatoes resistant to Phytophthora infestans, this oomycete is still economically the most important pathogen of potato worldwide. The correlation of high levels of resistance to late blight with a long vegetation period is one of the bottlenecks for progress in breeding resistant cultivars of various maturity types. Solanum phureja was identiWed as a source of eVective late blight resistance, which was transferred to the cultivated gene pool by interspeciWc crosses with dihaploids of Solanum tuberosum. A novel major resistance locus, Rpiphu1, derived most likely from S. phureja and conferring broad-spectrum resistance to late blight, was mapped to potato chromosome IX, 6.4 cM proximal to the marker GP94. Rpi-phu1 was highly eVective in detached leaXet, tuber slice and whole tuber tests during 5 years of quantitative phenotypic assessment. The resistance did not show signiWcant correlation with vegetation period length. Our Wndings provide a well-characterized new source of resistance for breeding early and resistant-to-P. infestans potatoes.

Tagging QTLs for late blight resistance and plant maturity from diploid wild relatives in a cultivated potato (Solanum tuberosum) background

“…Recently, the functionality of R1 both in foliage and in tubers, and foliage speciWcity of R3a have been conWrmed, and for another investigated R gene Rpi-abpt foliage speciWcity has been shown (Park et al 2005). A similar study was performed for Rpi-phu1 of S. phureja, which was shown to be highly eVective both in foliage and in tubers (Jliwka et al 2006). Tuber resistance assessment is very important while introducing a novel source of resistance to P. infestans into a breeding program because the relation of potato tuber and foliage resistance is a genotype-dependent trait.…”

“…Four tetraploid Polish cultivars were used as resistance standards: Irys (susceptible in foliage and in tubers), Sokói (susceptible in foliage and moderately resistant in tubers), Bzura (resistant in foliage and susceptible in tubers) and Meduza (resistant in leaXets and moderately resistant in tubers). Additionally, we used two diploid potato hybrids DG 94-15 with resistant foliage and DG 94-668 with resistant tubers (Jliwka et al 2006).…”

Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum

“…Examples are S. pinnatisectum (Kuhl et al 2001) and S. bulbocastanum (Naess et al 2000;Song et al 2003; Van der Vossen et al 2003 andPark et al 2005) from Mexico and S. berthaultii (Rauscher et al 2006;Ewing et al 2000), S. microdontum (Sandbrink et al 2000), S. mochiquense (Smilde et al 2005) and S. paucissectum (Villamon et al 2005) from Andean countries. In addition, the cultivated diploid S. phureja has been described to contain valuable resistance (Ghislain et al 2001;Sliwka et al 2006). The presence of late blight Rgenes in the above-mentioned species indicates that wild and so-called primitive germplasm is a rich source for novel R-genes that may be exploited in breeding programs.…”

R2-like Gene Contributes to Resistance to Phytophthora infestans in Polish Potato Cultivar Bzura

“…Recently, the number of potentially durable R genes, like RB/Rpi-blb1, Rpi-blb3, Rpi-phu1, Rpi-vnt1.1, Rpi-rzc1, Rpisto1, and Rpi-Smira2, were found (Lokossou et al 2009;Jo 2013;Pel et al 2009;Song et al 2003;Śliwka et al 2006;Śliwka et al 2012;Vleeshouwers et al 2008). Potato resistance breeding will greatly benefit from the using of these promising R genes.…”

“…Isolate MP 324 collected in 1997 in Poland is highly aggressive, metalaxyl resistant and is of A1 mating type. This isolate has frequently been used in late blight resistance tests Śliwka et al 2006, 2007.…”