The Novel Role of hnRNP UL1 in Human Cell Nucleoli

Cichocka, Marlena; Karlik, Anna; Plewka, Patrycja; Gawade, Kishor; Stępień, Agata; Świergiel, Patrycja; Gadgil, Ankur; Raczyńska, Katarzyna Dorota

doi:10.7150/ijbs.75084

Cited by 2 publications

(2 citation statements)

References 67 publications

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“…In addition, Hong et al found that HNRNPUL1 down-regulates the expression of PARP1 by controlling gene transcription and increases cellular sensitivity to double-strand breaks (DSBs) 48 . Cells silencing HNRNPUL1 were shown to be more sensitive to DNA damage 49 . HNRNPUL1 suppresses cisplatin (CDDP) sensitivity of esophageal squamous cell carcinoma cells by regulating the formation of MAN1A2, a cyclic RNA (circRNA) that suppresses CDDP sensitivity 25 .…”

Section: Discussionmentioning

confidence: 99%

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression

Long¹,

Ren²,

Wei³

et al. 2023

Int. J. Med. Sci.

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N4-acetylcytidine (ac4C) is a lately discovered nucleotide modification that has been shown to be closely implicated in cancer. N-acetyltransferase10(NAT10) acts as an enzyme that regulates mRNA acetylation modifications. Currently, the role of NAT10-mediated RNA acetylation modification in cervical cancer remains to be elucidated. On the basis of transcriptome analysis of TCGA and GEO open datasets (GSE52904, GSE29570, GSE122697), NAT10 is upregulated in cervical cancer tissues and correlated with poor prognosis. Knockdown of NAT10 suppressed the cell proliferation, invasion, and migration of cervical cancer cells. The in vivo oncogenic function of NAT10 was also confirmed in xenograft models. Combined RNA-seq and acRIP-seq analysis revealed HNRNPUL1 as the target of NAT10 in cervical cancer. NAT10 positively regulate HNRNPUL1 expression by promoting ac4C modification and stability of HNRNPUL1 mRNA. Furthermore, depletion of HNRNPUL1 suppressed the cell division, invasion, and migration of cervical cancer. HNRNPUL1 overexpression partially restored cellular function in cervical cancer cells with NAT10 knockdown. Thus, this study demonstrates that NAT10 contributes to cervical cancer progression by enhancing HNRNPUL1 mRNA stability via ac4C modification, and NAT10-ac4C-HNRNPUL1 axis might be a potential target for cervical cancer therapy.

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Section: Discussionmentioning

confidence: 99%

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression

Long¹,

Ren²,

Wei³

et al. 2023

Int. J. Med. Sci.

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“…Strikingly nucleolar association of some of them [hnRNPs K, G (RBMX), and A2/B1] is enhanced when transcription is inhibited ( Andersen et al, 2002 ). In addition, one recent study used immunofluorescence to show a colocalization of hnRNP UL1 with nucleolin, the major nucleolar protein, in HeLa cells ( Cichocka et al, 2022 ). However, it is worth mentioning that immunogold electron or immunofluorescence microscopy did not show any labeling of hnRNP C and A2/B1 in the nucleolus of human cells ( Romero et al, 1998 ; Friend et al, 2008 ), the discrepancy with the proteomic data likely coming from the different sensitivity in the methods used.…”

Section: Localization Expression and Regulationmentioning

confidence: 99%

hnRNPs: roles in neurodevelopment and implication for brain disorders

Tilliole,

Fix,

Godin

2024

Front. Mol. Neurosci.

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Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a family of multifunctional RNA-binding proteins able to process nuclear pre-mRNAs into mature mRNAs and regulate gene expression in multiple ways. They comprise at least 20 different members in mammals, named from A (HNRNP A1) to U (HNRNP U). Many of these proteins are components of the spliceosome complex and can modulate alternative splicing in a tissue-specific manner. Notably, while genes encoding hnRNPs exhibit ubiquitous expression, increasing evidence associate these proteins to various neurodevelopmental and neurodegenerative disorders, such as intellectual disability, epilepsy, microcephaly, amyotrophic lateral sclerosis, or dementias, highlighting their crucial role in the central nervous system. This review explores the evolution of the hnRNPs family, highlighting the emergence of numerous new members within this family, and sheds light on their implications for brain development.

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The Novel Role of hnRNP UL1 in Human Cell Nucleoli

Cited by 2 publications

References 67 publications

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression

hnRNPs: roles in neurodevelopment and implication for brain disorders

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