The Novel Small Leucine-rich Protein Chondroadherin-like (CHADL) Is Expressed in Cartilage and Modulates Chondrocyte Differentiation

Abstract

Background: Collagen-binding proteins regulate tissue-specific extracellular matrices. Results: CHADL is enriched in cartilage, binds collagen, and modulates the chondrocyte phenotype. Conclusion: CHADL regulates the chondrocyte microenvironment. Significance: Characterizing novel collagen-associated proteins is crucial to understand the constitution and function of specialized extracellular matrices.

From Whole Gene Deletion to Point Mutations ofEP300-Positive Rubinstein-Taybi Patients: New Insights into the Mutational Spectrum and Peculiar Clinical Hallmarks

“…Two genes ( miR1281 and miR6889 ) are provisional sequences possibly encoding for microRNAs, whereas EP300‐AS1 generates a long noncoding RNA. Protein coding genes includes: (1) ZC3H7B , a nuclear protein with a role in nuclear export of cytoplasmic poly(A)‐binding protein (PABP‐C1) [Harb et al., ]; (2) L3MBTL2 (MIM #611865), an integral component of Polycomb repressive complexes essential to compact chromatin and promote transcriptional repression of target genes [Trojer et al., ]; (3) CHADL (MIM #616236), encoding chondroadherin‐like, a small leucine‐rich protein expressed in the extracellular matrix of cartilage cells [Tillgren et al., ]; (4) RANGAP1 , codifying for the GTPase activator of the nuclear Ras‐related regulatory protein Ran, able to regulate the shuttle activity between nucleus and cytoplasm for proteins and ribonucleoproteins [Bischoff et al., ]; (5) TEF , coding for a bZIP transcription factor whose expression is diffused in adult tissues but restricted to the anterior pituitary during embryonic development in combination with the appearance of thyroid‐stimulating hormone beta (TSHB) [Drolet et al., ]; and (6) TOB2 gene, whose product belongs to the BTG/Tob family of proteins implicated in transcription, mRNA turnover and regulation of cell cycle progression, and contributing to cell type specific activities in different tissues [Winkler GS, ] ( A ). Histograms derived from qPCR reporting the relative DNA copy number of the two investigated regions (probe 1 in red/dark and probe 2 in pink/light) in proband, mother, and father compared with controls.…”

Proteomic analysis of aqueous humor proteins associated with cataract development

“…As shown in Table S5, 77 proteins were changed in common between each kind of patient and controls, among which 17 proteins were synchronously down-regulated and 38 proteins were synchronously upregulated in patients either with high myopia or after glaucoma surgery, or after vitrectomy surgery. Out of the 17 down-regulated proteins, chondroadherin-like protein (CHADL) was the most decreased one, which was recently reported to have a negative regulatory role and could possibly ensure the formation of a stable extracellular matrix [27]. Calsyntenin-1 was also decreased, and its decrease has been reported to be involved in proliferative diabetic retinopathy via differentiating vitreous proteomes.…”

Identification of molecular pathways and candidate genes associated with cocks’ comb size trait by genome-wide transcriptome analysis

“…Previous works also suggest that comb growth is closely related to bone deposition, and the identified BMP2 influences both comb growth and bone deposition as a candidate gene 15 . Another gene chondroadherin-like ( CHADL ) is expressed in cartilage and can modulate chondrocyte differentiation 37 . The expression level of CHADL was higher in the BC than the SC group, suggesting that this gene has a positive role in comb growth by mitigating cartilage metabolism.…”