The Novel Use of a Combination of Sonication and Vacuum Infiltration in Agrobacterium-mediated Transformation of Kidney Bean (Phaseolus vulgaris L.) with lea Gene

GENETIC TRANSFORMATION AND REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) USING Agrobacterium SYSTEM

“…The lack of an efficient regeneration system for common bean is a major bottleneck to genetically transform this species. Plant biotechnology, and conventional breeding methods, could facilitate bean improvement since resistance to biotic and abiotic stress could be increased (Liu et al, 2005). Nevertheless, efficient in vitro culture system that results in efficient regeneration is an essential requirement for improvement of common bean through genetic transformation (Svetleva et al, 2003;Kwapata et al, 2010).…”

Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes

“…However, recent progress has increased the feasibility of transformation as a tool in Phaseolus 7 . Liu et al 8 developed a protocol based on the combination of sonication and vacuum infiltration to transform common bean with a late embryogenesis abundant gene, conferring abiotic stress tolerance. As the efficiency is 12%, this method looks very promising, and should be further evaluated.…”

GENETIC TRANSFORMATION OF COMMON BEANS (Phaseolus vulgaris L.) CALLI BY BIOLISTIC GUN AND VIA Agrobacterium tumefaciens

“…Researchers in Brazil, used particle bombardment protocol to insert bar gene for herbicide resistance and virus resistance (RNAi) into the pinto bean V. olathe (Aragão and Faria, 2009). Researchers in Japan inserted the lea gene for drought tolerance in kidney beans using sonication and vacuum infiltration (Liu et al, 2005).…”