The Nuclear Maturation and Embryo Development of Mice Germinal Vesicle Oocytes with and without Cumulus Cell after Vitrification

Nikseresht, Mohsen; Toori, Mehdi Akbartabar; Rasti, Tahere; Kashani, Iraj Ragerdi; Mahmoudi, Reza

doi:10.7860/jcdr/2015/8856.5421

Cited by 13 publications

(9 citation statements)

References 32 publications

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“…Although a previous study reports that cumulus cells do not contribute to oocyte survival after freezing 25 , accumulating data suggest that cumulus cells and their extracellular matrix promote fertilization 26 27 . We found that oocytes surrounded by fully intact or partial cumulus layers had a higher fertilization rate than denuded oocytes, which is consistent with previous results 28 . Based on these findings, we conclude that the presence of cumulus layers promotes oocyte survival after freezing.…”

Section: Discussionsupporting

confidence: 93%

High survival of mouse oocytes using an optimized vitrification protocol

Zhou

Wang

Niu

et al. 2016

Sci Rep

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The method of vitrification has been widely used for cryopreservation. However, the effectiveness of this method for mammalian oocytes could be improved by optimizing each step of the process. In the present study, we tested the effects of varying several key parameters to determine the most effective protocol for mouse oocyte vitrification. We found that cryoprotectant containing ethylene glycol and dimethylsulfoxide plus 20% fetal calf serum produced the highest rates of oocyte survival, fertilization, and blastocyst formation. The duration and temperature of oocyte exposure to vitrification and thawing solutions influenced survival rate. The presence of cumulus cells surrounding oocytes and the incubation of thawed oocytes in Toyoda-Yokoyama-Hosoki medium also increased oocyte survival. Open pulled straw and nylon loop methods were more effective than the mini-drop method. Finally, the combination of these improved methods resulted in better spindle morphology when compared to the unimproved methods. These results demonstrate that the outcomes of mouse oocyte vitrification can be improved by a suitable combination of cryopreservation methods, which could be applied to future clinical research with human oocytes.

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Section: Discussionsupporting

confidence: 93%

High survival of mouse oocytes using an optimized vitrification protocol

Zhou

Wang

Niu

et al. 2016

Sci Rep

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“…However, compared to conventional ovarian stimulation and in vivo maturation of oocytes, reduced pregnancy rates yielded with IVM have precluded its application on a large scale; IVM only supposes 0.1% of the total in vitro cycles, according to the European Society of Human Reproduction and Embryology (49). Nevertheless, IVM is a promising alternative treatment option for many patients unsuitable for conventional IVF treatment, such as patients with severe polycystic ovary syndrome or cancer (50, 51). In addition, by avoiding controlled ovarian stimulation, the IVM procedure eliminates the need for frequent ultrasound monitoring and could be cheaper than conventional IVF (52).…”

Section: Discussionmentioning

confidence: 99%

CB₁cannabinoid receptor drives oocyte maturation and embryo development via PI3K/Akt and MAPK pathways

et al. 2017

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Endocannabinoids have been recognized as mediators of practically all reproductive events in mammals. However, little is known about the role of this system in oocyte maturation. In a mouse model, we observed that activation of cannabinoid receptor 1 (CB) during oocyte maturation modulated the phosphorylation status of Akt and ERK1/2 and enhanced the subsequent embryo production. In the absence of CB, oocyte maturation was impaired and embryo development delayed. Cannabinoid receptor 2 (CB) was unable to rescue these effects. Finally, we confirmed abnormal oocyte maturation rather than impaired embryonic transport through the oviduct in CB knockouts. Our data suggest that cannabinoid agonists may be useful maturation supplements. For fertilization patients intolerant to gonadotropins, this could be a promising and only option.-López-Cardona, A. P., Pérez-Cerezales, S., Fernández-González, R., Laguna-Barraza, R., Pericuesta, E., Agirregoitia, N., Gutiérrez-Adán, A., Agirregoitia, E. CB cannabinoid receptor drives oocyte maturation and embryo development PI3K/Akt and MAPK pathways.

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“…Fertilizability preservation was undoubtedly proved by the same group that recently obtained live rats from vitrified COCs fertilized in vitro with cryopreserved sperm (Fujiwara et al , 2017). Similarly, vitrified immature mouse oocytes had higher survival, maturation and embryo developmental rates, with respect to the denuded vitrified counterpart (Nikseresht et al , 2015). CCs also seemed to maintain the fertilization ability in vitrified bovine oocytes, as demonstrated by comparing blastocyst formation and cleavage rates from vitrified DOs and COCs.…”

Section: Introductionmentioning

confidence: 97%

Human cumulus cell sensitivity to vitrification, an ultrastructural study

Taghizabet

Khalili

Anbari

et al. 2018

Zygote

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SummaryCumulus cells (CCs) play an important role in the regulation of female gamete development, meiotic maturation, oocyte-sperm interaction, capacitation and acrosome reaction. However, their role in maintaining oocyte competence after vitrification is unclear as controversial data on their protecting action against oocyte cryoinjuries are available. Here we described the effects of vitrification on the ultrastructure of human CCs collected from women undergoing assisted reproductive technologies (ARTs). In total, 50 patches of CCs, sampled from high-quality human cumulus-oocyte complexes, were randomly allocated into two groups after patient informed consent: 1, fresh CCs (controls, n = 25); 2, vitrified CCs (n = 25). Samples were then prepared and observed by transmission electron microscopy. In fresh CCs, in which small cell clusters were visible, cell membranes were joined by focal gap junctions. Microvilli were rare and short. Nuclei, mitochondria, smooth endoplasmic reticulum (SER), Golgi apparatus and lipid droplets appeared well preserved; vacuoles were scarce. After vitrification, we observed two populations of CCs: light CCs, with a smooth appearance and few short microvilli; and dark CCs, with numerous and long microvilli. In both, most of the organelles appeared similar to those of fresh CCs. Lipid droplets were denser and more numerous, with respect to fresh CCs. They were mainly located in the peri-nuclear and sub-plasmalemmal regions. Numerous packed electron-negative vacuoles were visible. The vitrification procedure did not cause alterations in the fine structure of major organelles, except for an increased amount of lipid droplets and vacuoles. This specific sensitivity of human CCs to vitrification should be considered during ARTs.

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The Nuclear Maturation and Embryo Development of Mice Germinal Vesicle Oocytes with and without Cumulus Cell after Vitrification

Cited by 13 publications

References 32 publications

High survival of mouse oocytes using an optimized vitrification protocol

High survival of mouse oocytes using an optimized vitrification protocol

CB₁cannabinoid receptor drives oocyte maturation and embryo development via PI3K/Akt and MAPK pathways

Human cumulus cell sensitivity to vitrification, an ultrastructural study

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The Nuclear Maturation and Embryo Development of Mice Germinal Vesicle Oocytes with and without Cumulus Cell after Vitrification

Cited by 13 publications

References 32 publications

High survival of mouse oocytes using an optimized vitrification protocol

High survival of mouse oocytes using an optimized vitrification protocol

CB1cannabinoid receptor drives oocyte maturation and embryo development via PI3K/Akt and MAPK pathways

Human cumulus cell sensitivity to vitrification, an ultrastructural study

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CB₁cannabinoid receptor drives oocyte maturation and embryo development via PI3K/Akt and MAPK pathways