The Nuclear Pore Regulates GAL1 Gene Transcription by Controlling the Localization of the SUMO Protease Ulp1

Texari, Lorane; Dieppois, Guennaëlle; Vinciguerra, Patrizia; Contreras, Mariana Pardo; Groner, Anna C.; Letourneau, Audrey; Stutz, Françoise

doi:10.1016/j.molcel.2013.08.047

Cited by 75 publications

(103 citation statements)

References 67 publications

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“…In addition, budding yeast Ulp1 was recently shown to desumoylate transcription factors on chromatin to facilitate transcriptional activation, providing an example of recruitment of Ulp1 targets to the nuclear pore (50). Interestingly, a subcomplex of the human nuclear pore including Nup133 transiently localizes to centromeres and contributes to their mitotic function (55).…”

Section: Discussionmentioning

confidence: 99%

Pli1PIAS1 SUMO Ligase Protected by the Nuclear Pore-associated SUMO Protease Ulp1SENP1/2

Nie

Boddy

2015

Journal of Biological Chemistry

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Background: SUMO conjugation to the proteome critically controls cell growth, but mechanisms for regulating SUMO ligases are poorly defined. Results: Desumoylation of the major fission yeast SUMO ligase by a nuclear pore-associated protease protects it from proteolysis. Conclusion: Desumoylation of a SUMO ligase antagonizes autoinhibition of the SUMO pathway.Significance: These data demonstrate cooperation between STUbL and Cdc48(p97) in proteasome-mediated degradation of SUMO conjugates.

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Section: Discussionmentioning

confidence: 99%

Pli1PIAS1 SUMO Ligase Protected by the Nuclear Pore-associated SUMO Protease Ulp1SENP1/2

Nie

Boddy

2015

Journal of Biological Chemistry

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“…For example, a recent study showed that Ulp1, an NPCassociated SUMO protease, accelerates derepression of the prototypical GAL1 gene, which migrates to NPCs during induction (Texari et al 2013). This scenario and others in which tDNAs associate with NPCs for reasons other than export are distinct because, in these cases, pretRNAs generated at the NPC might venture elsewhere in the nucleus before subsequent export.…”

Section: Potential Advantages Of Tdna Transcription At Npcsmentioning

confidence: 99%

“…After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http:// creativecommons.org/licenses/by-nc/4.0/. initial transcriptional induction as well as transcriptional reactivation (Hampsey et al 2011;Texari et al 2013).…”

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confidence: 99%

Coordination of tRNA transcription with export at nuclear pore complexes in budding yeast

Chen

Gartenberg

2014

Genes Dev.

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tRNAs are encoded by RNA polymerase III-transcribed genes that reside at seemingly random intervals along the chromosomes of budding yeast. Existing evidence suggests that the genes congregate together at the nucleolus and/or centromeres. In this study, we re-examined spatial and temporal aspects of tRNA gene (tDNA) expression. We show that tDNA transcription fluctuates during cell cycle progression. In M phase, when tRNA synthesis peaks, tDNAs localize at nuclear pore complexes (NPCs). Docking of a tDNA requires the DNA sequence of the contacted gene, nucleoporins Nup60 and Nup2, and cohesin. Characterization of mutants that block NPC localization revealed that docking is a consequence of elevated tDNA transcription. NPC-tDNA contact falters in the absence of the principal exportin of nascent tRNA, Los1, and genetic assays indicate that gating of tDNAs at NPCs favors cytoplasmic accumulation of functional tRNA. Collectively, the data suggest that tDNAs associate with NPCs to coordinate RNA polymerase III transcription with the nuclear export of pre-tRNA. The M-phase specificity of NPC contact reflects a regulatory mechanism that may have evolved, in part, to avoid collisions between DNA replication forks and transcribing RNA polymerase III machinery at NPCs.[Keywords: tRNA gene; RNA polymerase III; nuclear pore complex; cohesin; cell cycle; Los1 exportin] Supplemental material is available for this article. Nuclear pore complexes (NPCs) form large aqueous channels through the nuclear envelope that permit transfer of materials between the nucleus and cytoplasm. In 1985, Blobel (1985 proposed the gene-gating hypothesis, which stipulated that transcriptionally poised genes associate with NPCs. In this way, tethered genes would be situated to export transcripts directly to the cytoplasm. The original gene-gating scheme also envisioned that genome packaging would influence the distribution of NPCs on the nuclear envelope. A contemporary spin on this idea posits that anchorage of genes to NPCs and other structures at the edge of the nucleus orchestrates the three-dimensional structure of the genome (Taddei and Gasser 2012).Substantial evidence for gating of RNA polymerase II genes has been obtained primarily from studies of the budding yeast Saccharomyces cerevisiae. Genome-wide approaches revealed that components of the NPC, known as nucleoporins or Nups, associate with many active genes (Casolari et al. 2004;Schmid et al. 2006). Focused analyses of representative examples showed that the genes moved from the nucleoplasm to the nuclear periphery upon induction in a Nup-dependent manner. That transcription and export might be coupled events emerged from studies showing that mRNA export machinery associated with transcribed genes and that the machinery was required for positioning the genes at NPCs (for review, see Dieppois and Stutz 2010). Whether gating of genes at NPCs affects their expression has been less certain. For example, one study showed that artificially targeting a reporter gene to NPCs increased transcri...

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“…In addition to the main function of the Nups in nucleocytoplasmic transport, new roles -especially in the regulation of gene expression -have recently been identified (Texari et al, 2013;Van de Vosse et al, 2013). Therefore, it is not surprising that aberrant functions of Nups and NPCs have been implicated in many and diverse human pathologies, including autoimmune diseases, viral infections, cardiomyopathies and various cancers (Capelson and Hetzer, 2009;Chow et al, 2012;Hatch and Hetzer, 2014;Simon and Rout, 2014).…”

Section: Perspectivesmentioning

confidence: 99%

The nuclear pore complex – structure and function at a glance

Kabachinski

Schwartz

2015

Journal of Cell Science

184

136

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Nuclear pore complexes (NPCs) are indispensable for cell function and are at the center of several human diseases. NPCs provide access to the nucleus and regulate the transport of proteins and RNA across the nuclear envelope. They are aqueous channels generated from a complex network of evolutionarily conserved proteins known as nucleporins. In this Cell Science at a Glance article and the accompanying poster, we discuss how transport between the nucleoplasm and the cytoplasm is regulated, what we currently know about the structure of individual nucleoporins and the assembled NPC, and how the cell regulates assembly and disassembly of such a massive structure. Our aim is to provide a general overview on what we currently know about the nuclear pore and point out directions of research this area is heading to.

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The Nuclear Pore Regulates GAL1 Gene Transcription by Controlling the Localization of the SUMO Protease Ulp1

Cited by 75 publications

References 67 publications

Pli1PIAS1 SUMO Ligase Protected by the Nuclear Pore-associated SUMO Protease Ulp1SENP1/2

Pli1PIAS1 SUMO Ligase Protected by the Nuclear Pore-associated SUMO Protease Ulp1SENP1/2

Coordination of tRNA transcription with export at nuclear pore complexes in budding yeast

The nuclear pore complex – structure and function at a glance

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