The nuclear protein Artemis promotes AMPK activation by stabilizing the LKB1–AMPK complex

AMPK: restoring metabolic homeostasis over space and time

“…AMPK has been proposed to play a role in UVB response previously (Wu et al, 2013a), and a recent careful analysis of genetic ablation of AMPK in the mouse skin showed defects in UVB response and general growth, though mTORC1 was a key target of AMPK in that process (Crane et al, 2021). Artemis, a protein involved in non-homologous end-joining DNA repairs, has been reported in multiple independent studies as a nuclear AMPK interactor (Chen et al, 2020;Nakagawa et al, 2012), which may relate to a recent finding that Kras LKB1-deficient lung cancers show specific defects in double-strand repair when compared with Kras tumors with other coincident mutations (Deng et al, 2021).…”

The transcriptional activity of Gli1 is negatively regulated by AMPK through Hedgehog partial agonism in hepatocellular carcinoma

“…AMPK is a heterotrimeric serine/threonine protein kinase composed of 3 subunits: a catalytic subunit (α), a scaffolding subunit (β) and an AMP-sensing subunit (γ). Its kinase activity is controlled by the AMP/ATP ratio and some upstream kinases, such as liver kinase B1 (LKB1), TGF-β-activated kinase 1 (TAK1) and Ca 2+ /calmodulin-dependent protein kinase kinase (CaMKK) (10–15). AMPK controls cell metabolism and growth in response to changes in nutrient availability by phosphorylating a variety of substrates in cells, including acetyl-CoA carboxylase (ACC), forkhead box O3 (FOXO3) and tuberous sclerosis complex 2 (TSC2) (16–18).…”