The nuclear reticulum in placental cells ofLilium regale is a part of the endomembrane system

Singh, Sangeeta; Lazzaro, Mark D.; Walles, Björn

doi:10.1007/bf01279471

Cited by 11 publications

(8 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2D) or as substantial expansions (Fig. 2C–F) forming a reticulum inside the nucleus, similar to the nuclear reticulum described by Singh et al. (1998).…”

Section: Resultssupporting

confidence: 79%

Early meiosis in Rhynchospora pubera L. (Cyperaceae) is marked by uncommon ultrastructural features

Martin

Andrade

Vanzela

2009

Cell Biology International

View full text Add to dashboard Cite

The family Cyperaceae has an unusual microsporogenesis in which tetrad formation does not occur. In addition, other cytological features are important, such as the occurrence of holokinetic chromosomes and post-reductional meiosis. We have examined the ultrastructural features of the pollen mother cell (PMC) of Rhynchospora pubera. Anthers of several sizes were analyzed using light and transmission electron microscopy. The PMC before meiosis presented a central nucleus and a regular profile of the nuclear envelope. During prophase I, the nucleus was in the abaxial region of the cell. This cellular polarization was accompanied by other marked ultrastructural features in the nuclear envelope. Morphological changes involved dilations of perinuclear cisterns and polarization of the nuclear pore complexes. The results show that polarization occurs in the initial phases of microsporogenesis in R. pubera, unlike other plant species.

show abstract

“…2D) or as substantial expansions (Fig. 2C–F) forming a reticulum inside the nucleus, similar to the nuclear reticulum described by Singh et al. (1998).…”

Section: Resultssupporting

confidence: 79%

Early meiosis in Rhynchospora pubera L. (Cyperaceae) is marked by uncommon ultrastructural features

Martin

Andrade

Vanzela

2009

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…Furthermore, because the structures in onion contain a cytoplasmic core, they are unlike the nuclear reticulum found in Lilium ovary placental cells, in which only the inner membrane of the nuclear envelope invaginates, so that the centers of these invaginations are continuous with the lumen of the ER rather than the cytoplasm (Singh and Walles, 1995;Singh et al, 1998).…”

Section: Nuclear Invaginations In Onion Are Similar To Structures Seementioning

confidence: 99%

“…The Plant Cell placental cells in Lilium ovaries contain a nuclear reticulum in which the inner nuclear membrane forms extensive tubular invaginations that penetrate through the center of the nucleus, the lumen of this reticulum being contiguous with the lumen of the ER (Singh and Walles, 1995;Singh et al, 1998). This type of structure differs from the shallow and narrow invaginations of both membranes of the nuclear envelope found in developing microspores of various species (Aldrich and Vasil, 1970; Bell, 1970, 1972;Li and Dickinson, 1987) and also differs from nuclear vacuoles, membrane-bound inclusions within the nucleus that are also generally found in meiotic cells (Sheffield et al, 1979;Karasawa and Ueda, 1983;Sangwan, 1986;Yi et al, 1994).…”

mentioning

confidence: 99%

Plant Nuclei Can Contain Extensive Grooves and Invaginations [W]

et al. 2000

View full text Add to dashboard Cite

Plant cells can exhibit highly complex nuclear organization. Through dye-labeling experiments in untransformed onion epidermal and tobacco culture cells and through the expression of green fluorescent protein targeted to either the nucleus or the lumen of the endoplasmic reticulum/nuclear envelope in these cells, we have visualized deep grooves and invaginations into the large nuclei of these cells. In onion, these structures, which are similar to invaginations seen in some animal cells, form tubular or planelike infoldings of the nuclear envelope. Both grooves and invaginations are stable structures, and both have cytoplasmic cores containing actin bundles that can support cytoplasmic streaming. In dividing tobacco cells, invaginations seem to form during cell division, possibly from strands of the endoplasmic reticulum trapped in the reforming nucleus. The substantial increase in nuclear surface area resulting from these grooves and invaginations, their apparent preference for association with nucleoli, and the presence in them of actin bundles that support vesicle motility suggest that the structures might function both in mRNA export from the nucleus and in protein import from the cytoplasm to the nucleus. INTRODUCTIONAlthough cellular function often requires maximization of surface area relative to volume, notably in organelles such as the endoplasmic reticulum (ER) and Golgi apparatus, traditional representations of the nucleus depict a rounded structure with little internal organization. Recently, however, the nuclei of animal cells have been found to show considerable spatial and structural organization at the chromosomal level. The discrete and comparatively stable territories that chromosomes occupy within the nucleus are separated by interchromosomal domains through which transcribed RNA and other macromolecules can diffuse (reviewed in Lamond and Earnshaw, 1998). Animal nuclei also sometimes deviate from the characteristic rounded shape. Deviations include a folded, grooved, or notched surface (Majno et al., 1969) and tubular invaginations. Electron microscopy has shown that these nuclear invaginations can penetrate deep into the nucleus and confirmed the presence of the double membrane of the nuclear envelope (Ellenberg et al., 1997;Fricker et al., 1997;Clubb and Locke, 1998). However, revelation of the full nature of nuclear invaginations in living animal cells has required confocal microscopy of fluorescent dyes (Fricker et al., 1997;Lui et al., 1998aLui et al., , 1998b or green fluorescent protein (GFP) fusion proteins targeted to the nuclear envelope (Ellenberg et al., 1997;Broers et al., 1999). The number and nature of invaginations vary from cell type to cell type, ranging from simple invaginations to intricate branched structures that can penetrate into and through the nucleus. Invaginations often associate with nucleoli (Fricker et al., 1997) and appear to be stable (Ellenberg et al., 1997;Broers et al., 1999). Nuclear grooves and invaginations substantially increase the surface area of the ...

show abstract

“…They re− sembled nuclear vacuoles of mitotic cells (Sheffield et al 1979;Yi et al 1994) and endoplasmic reticulum elements trapped inside nuclei. It is believed that such struc− tures are a part of the membrane system in the cytoplasm (Singh and Walles 1995;Singh et al 1998). Extended endoplasmic reticulum comprising transverse and lon− gitudinal cisternae was observed in the direct proximity of cell nuclei in C. quitensis.…”

Section: Discussionmentioning

confidence: 98%

“…Different visualization techniques have been used to analyze atypical characteristics of cell nuclei, including their ability to create grooves and invaginations (Singh et al 1998;Collings et al 2000;Meier 2001 Determining the location of insoluble polysaccharides. -Insoluble poly− saccharides were determined by the Periodic acid−Schiff (PAS) reaction (Pearse 1985) in leaf blade segments fixed in Carnoy's solution (96% ethanol and acetic acid v/v, 3:1) at room temperature for 12 hours.…”

Section: Introductionmentioning

confidence: 99%

Cold stress effects on organelle ultrastructure in polar Caryophyllaceae species

Giełwanowska¹,

Pastorczyk²,

Lisowska³

et al. 2014

Polish Polar Research

View full text Add to dashboard Cite

This study investigated leaf mesophyll cells of Caryophyllaceae plants growing in polar regions -Cerastium alpinum and Silene involucrata from the Hornsund region of Spitsbergen island (Svalbard Archipelago, Arctic), and Colobanthus quitensis from the Ad− miralty Bay region on King George Island (South Shetland Islands, West Antarctic). Ultra− structural changes were analyzed in mesophyll protoplasts of plants growing in natural Arctic and Antarctic habitats and plants grown in a greenhouse, including plants exposed to short−term cold stress under semi−controlled conditions. Cell organelles of plants growing in natural polar habitats and greenhouse−grown plants were characterized by significant mor− phological plasticity. Chloroplasts of plants studied in this work formed variously shaped pro− trusions and invaginations that visibly increased the contact area between adjacent cell com− partments and reduced the distance between organelles. S. involucrata plants grown under greenhouse conditions, tested by us in this work, were characterized by highly dynamic cell nuclei with single or multiple invaginations of the nuclear membrane and the presence of channels and cisternae filled with cytoplasm and organelles. Crystalline inclusion proteins were observed in the cell nuclei of C. quitensis between nuclear membranes and in the direct proximity of heterochromatin. Our study revealed significant conformational dynamics of organelles, manifested by variations in the optical density of matrices, membranes and enve− lopes, in particular in C. quitensis, which could suggest that the analyzed Caryophyllaceae taxa are well adapted to severe climate and changing conditions in polar regions.

show abstract

The nuclear reticulum in placental cells ofLilium regale is a part of the endomembrane system

Cited by 11 publications

References 61 publications

Early meiosis in Rhynchospora pubera L. (Cyperaceae) is marked by uncommon ultrastructural features

Early meiosis in Rhynchospora pubera L. (Cyperaceae) is marked by uncommon ultrastructural features

Plant Nuclei Can Contain Extensive Grooves and Invaginations [W]

Cold stress effects on organelle ultrastructure in polar Caryophyllaceae species

Contact Info

Product

Resources

About