The nucleic acid ligand. A new tool for molecular recognition

McGown, Linda B.; Joseph, Melissa J.; Pitner, J. Bruce; vonk, Glenn P.; Linn, C. P.

doi:10.1021/ac00117a002

Cited by 79 publications

(38 citation statements)

References 8 publications

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“…Studies with additional cytokines are ongoing. With the increase in synthetic receptors such as aptamers (McGown et al, 1995) as well as ongoing work in designing synthetic peptides with specific receptor capability (Enander et al, 2004;Rzepecki et al, 2004), there should be additional suitable protein trapping agents for microdialysis sampling.…”

Section: Summary and Future Perspectivesmentioning

confidence: 99%

Natural and synthetic affinity agents as microdialysis sampling mass transport enhancers: Current progress and future perspectives

Duo

Fletcher

Stenken

2006

Biosensors and Bioelectronics

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Section: Summary and Future Perspectivesmentioning

confidence: 99%

Natural and synthetic affinity agents as microdialysis sampling mass transport enhancers: Current progress and future perspectives

Duo

Fletcher

Stenken

2006

Biosensors and Bioelectronics

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“…The "discovery" of aptamers in 1990 has opened a new scenario in the development of RNA/DNA-based assays for different molecules (Tuerk and Gold, 1990;McGown et al, 1995), but a further advance in nucleic acid nanotechnology was made in 1994 with the first application of a DNA with a catalytic function (DNAzyme) (Breaker and Joyce, 1994). An example of DNAzyme is a single-stranded guanine-rich nucleic acid that has been reported for binding a hemin to yield a DNAzyme possessing peroxidase-like activity.…”

Section: Aptazymes and Aptamer-based Machinesmentioning

confidence: 99%

Aptamer‐Based Bioanalytical Assays: Amplification Strategies

Tombelli

Minunni

Mascini

2008

Aptamers in Bioanalysis

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“…Initially, biological reagents such as antibodies, enzymes, and membrane-bound receptors were used for sensor development, 24 since these species were available in high purity, or could be extracted from living tissue. More recently, species such as regulatory proteins, 25 RNA, 26 DNA 27 and nucleic acid aptamers 28 have been exploited for optical sensor development. In these cases, the development of new optical biosensors had to wait for advances in molecular biology and for the emergence of new synthetic tools, such as solid-phase synthesis of DNA, before the biomolecules could be obtained.…”

Section: Historymentioning

confidence: 99%

Fluorescence‐Based Biosensors

Brennan

2000

Encyclopedia of Analytical Chemistry

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Biosensors, as defined by Biosensors and Bioelectronics, are analytical devices “incorporating a biological material, a biologically derived material or biomimetic intimately associated with or integrated within a physicochemical transducer or transducing microsystem”. Fluorescence‐based biosensors are those devices that derive an analytical signal from the fluorescence emission process. Such biosensors may be used for a wide variety of tasks, including: detection of compounds of biomedical, 1 environmental 2 or defense interest, 3 on‐line monitoring for process control, 4 monitoring of foodstuffs, 5 selective detection of compounds undergoing a chemical separation, 6 and screening of drug compounds. 7 Advantages of such devices include: 8 high selectivity; rapid response times; reusability; amenability to remote analysis; and immunity from electrical interferences. The selective nature of the complexation between the biomolecule and the analyte, combined with the small size of the device and the advantages of total internal reflection (TIR)‐based spectroscopy, 9 also results in an ability to measure analytes in complex matrixes. Such samples may include highly scattering systems such as milk or whole blood, 10 or relatively inaccessible locations such as groundwater wells, or even intracellular environments. 11 The key limitation of such devices mainly centers around the poor stability of biological compounds, which can lead to a substantial drift in instrument response over time. There is also the potential for interferences related to biological autofluorescence, and the analyte‐dependent sensitivity and limit of detection (LOD) for sensors, which rely on the nature of both the protein and the fluorescent probe utilized. Finally, in cases where immunological reagents are used, the devices can show a lack of reversibility, and may operate only as a “one‐shot” screen, without the potential for continuous, quantitative analysis.

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The nucleic acid ligand. A new tool for molecular recognition

Cited by 79 publications

References 8 publications

Natural and synthetic affinity agents as microdialysis sampling mass transport enhancers: Current progress and future perspectives

Natural and synthetic affinity agents as microdialysis sampling mass transport enhancers: Current progress and future perspectives

Aptamer‐Based Bioanalytical Assays: Amplification Strategies

Fluorescence‐Based Biosensors

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