The nucleolus: an old factory with unexpected capabilities

Olson, Matthew S.; Dundr, Miroslav; Szebeni, Attila

doi:10.1016/s0962-8924(00)01738-4

Cited by 365 publications

(318 citation statements)

References 56 publications

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“…Indeed, an increasing data showed that many proteins unrelated to ribosome assembly, including viral proteins encoded by DNA and RNA viruses [16], were detected in the nucleolus. Thus, many non-traditional functions of the nucleolus have been proposed [5][6][7]9]. These include signal recognition particle assembly, small RNA modification, RNA editing, telomerase maturation, nuclear export, cell cycle control, and stress sensor [17][18][19][20][21][22].…”

Section: Plurifunctionality Of the Nucleolusmentioning

confidence: 99%

“…However, to understand the structure and function of nucleolus then took more than one century. Till 1960s, two major progresses in nucleolus research have been accumulated and summarized as follows: (i) the nucleolus is membrane-less structure and appears fibril and granule, which are recognized as a tripartite structure, including the dense fibrillar component (DFC), the fibrillar center (FC), and the granular component (GC), under an electron microscope [4][5][6] ( Figure 1B); and (ii) it is the site for pre-rRNA transcription and processing and for ribosome subunit assembly and thus known as a "factory of ribosome" [5][6][7][8]. On the other hand, the dynamic disappearance and reappearance of nucleoli in mitotic cells were described in 1893 and 1894 [4].…”

Section: Introductionmentioning

confidence: 99%

“…At the same period of time, new functions, of the nucleolus, in addition to the ribosome biogenesis, which are called non-traditional or non-conventional functions, were emerged [5,9,10]. More importantly, in the past few years, a new era of bioinformatic analyses of the nucleolus began [11,12].…”

Section: Introductionmentioning

confidence: 99%

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The nucleolus: reviewing oldies to have new understandings

Lee

Lai

2006

Cell Res

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Section: Plurifunctionality Of the Nucleolusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The nucleolus: reviewing oldies to have new understandings

Lee

Lai

2006

Cell Res

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“…In the 1960s, it was demonstrated that nucleoli are the site of ribosome biogenesis. This function gives rise to their characteristic ultrastructural organization into three distinct regions visible by electron microscopy: the fibrillar center, the dense fibrillar component, and the granular component (Melese and Xue, 1995;Scheer and Hock, 1999;Olson et al, 2000). Ribosome biogenesis is a very complex process that involves the synthesis and assembly of four different mature rRNA molecules and of 80 different proteins.…”

Section: Introductionmentioning

confidence: 99%

Functional Proteomic Analysis of Human Nucleolus

Scherl

Couté

Déon

et al. 2002

MBoC

445

378

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The notion of a “plurifunctional” nucleolus is now well established. However, molecular mechanisms underlying the biological processes occurring within this nuclear domain remain only partially understood. As a first step in elucidating these mechanisms we have carried out a proteomic analysis to draw up a list of proteins present within nucleoli of HeLa cells. This analysis allowed the identification of 213 different nucleolar proteins. This catalog complements that of the 271 proteins obtained recently by others, giving a total of ∼350 different nucleolar proteins. Functional classification of these proteins allowed outlining several biological processes taking place within nucleoli. Bioinformatic analyses permitted the assignment of hypothetical functions for 43 proteins for which no functional information is available. Notably, a role in ribosome biogenesis was proposed for 31 proteins. More generally, this functional classification reinforces the plurifunctional nature of nucleoli and provides convincing evidence that nucleoli may play a central role in the control of gene expression. Finally, this analysis supports the recent demonstration of a coupling of transcription and translation in higher eukaryotes.

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“…In eukaryotes, ribosome biogenesis takes place primarily in the nucleolus (Olson et al 2000;Pederson and Politz 2000). The process starts with the transcription of the primary transcripts (35S pre-rRNA and pre-5S rRNA in yeast) by distinct RNA polymerases (Pol I and Pol III, respectively) and requires the import of most ribosomal proteins (r-proteins) from the cytoplasm (Nomura et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Npa1p is an essential trans-acting factor required for an early step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae

Rosado

Cruz²

2004

RNA

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Ribosome biogenesis requires >100 nonribosomal proteins, which are associated with different preribosomal particles. The substrates, the interacting partners, and the timing of action of most of these proteins are largely unknown. To elucidate the functional environment of the putative ATP-dependent RNA helicase Dbp6p from Saccharomyces cerevisiae, which is required for 60S ribosomal subunit assembly, we have previously performed a synthetic lethal screen and thereby revealed a genetic interaction network between Dbp6p, Rpl3p, Nop8p, and the novel Rsa3p. In this report, we extended the characterization of this functional network by performing a synthetic lethal screen with the rsa3 null allele. This screen identified the so far uncharacterized Npa1p (YKL014C). Polysome profile analysis indicates that there is a deficit of 60S ribosomal subunits and an accumulation of half-mer polysomes in the slowly growing npa1-1 mutant. Northern blotting and primer extension analysis shows that the npa1-1 mutation negatively affects processing of all 27S pre-rRNAs and the normal accumulation of both mature 25S and 5.8S rRNAs. In addition, 27SA 2 pre-rRNA is prematurely cleaved at site C 2 . Moreover, GFP-tagged Npa1p localizes predominantly to the nucleolus and sediments with large complexes in sucrose gradients, which most likely correspond to pre-60S ribosomal particles. We conclude that Npa1p is required for ribosome biogenesis and operates in the same functional environment of Rsa3p and Dbp6p during early maturation of 60S ribosomal subunits.

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The nucleolus: an old factory with unexpected capabilities

Cited by 365 publications

References 56 publications

The nucleolus: reviewing oldies to have new understandings

The nucleolus: reviewing oldies to have new understandings

Functional Proteomic Analysis of Human Nucleolus

Npa1p is an essential trans-acting factor required for an early step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae

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