The nucleolus of the maternal gamete is essential for life

Lefèvre, Brigitte

doi:10.1002/bies.20774

Cited by 20 publications

(13 citation statements)

References 12 publications

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“…We have also taken into account the chromatin configuration as already described in GV oocytes (Bouniol-Baly et al, 1999;Zuccotti et al, 2002) where the chromatin may be dispersed in the nucleus (NSN) or condensed around the nucleolus (SN). The SN oocytes are considered as more ready to resume meiosis and more able to be fertilized and to have a good embryo development (for review see Lefèvre, 2008).…”

Section: Immunolocalization Of the Three Caspases During The Differenmentioning

confidence: 99%

Caspase‐2_L, caspase‐9, and caspase‐3 during in vitro maturation and fragmentation of the mouse oocyte

Arnault

Tosca

Courtot

et al. 2008

Developmental Dynamics

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Several studies have shown that apoptotic pathways control fragmentation of unfertilized ovulated oocyte, induced by doxorubicin. But very few have investigated the basis of this process, from prophase I to later stages. Our results revealed the presence of caspase-2 L , caspase-9, and caspase-3 in their zymogen and cleaved forms in the oocyte before meiosis resumption. Caspase-2 L and caspase-9 were detected in the nucleus of GV-oocytes in a distribution related to chromatin configuration. The inhibition of caspase activity by Z-VAD-fmk accelerated the transition from metaphase I to metaphase II, and caspase-9 and caspase-3 were detected along the meiotic spindle. Surprisingly, Western blot analysis revealed that the three cleaved caspases were present in similar amounts in healthy and fragmented oocytes and caspase inhibition did not prevent doxorubicin-induced apoptosis. Our results suggest that, if cleaved, caspases may be dispensable for final oocyte death and they could be involved in regulating the maturation process.

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Section: Immunolocalization Of the Three Caspases During The Differenmentioning

confidence: 99%

Caspase‐2_L, caspase‐9, and caspase‐3 during in vitro maturation and fragmentation of the mouse oocyte

Arnault

Tosca

Courtot

et al. 2008

Developmental Dynamics

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“…The presence of a nucleolus is not necessary for the spermatozoon, because, after fecundation, the nucleoli of the male and female pronuclei in the zygote are both of maternal origin. Recent studies have suggested that the maternal nucleolus, associated with other nucleoplasmic products, is essential for embryonic development [57]. Peripheral nucleoli were also reported by [58] where the nucleolar position in the nucleus was linked to the NORs location.…”

Section: Discussionmentioning

confidence: 88%

Morphological Changes of Mammalian Nucleoli during Spermatogenesis and Their Possible Role in the Chromatoid Body Assembling

2012

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Chromatoid body (CB) is a typical cytoplasmic organelle of germ cells, and it seems to be involved in RNA/protein accumulation for later germ-cell differentiation. Despite most of the events in mammals spermatogenesis had been widely described in the past decades and the increase in the studies related to the CB molecular composition and physiology, the origins and functions of this important structure of male germ cells are still unclear. The aims of this study were to describe the nucleolar cycle and also to find some relationship between the nucleolar organization and the CB assembling during the spermatogenesis in mammals. Cytochemical and cytogenetics analysis showed nucleolar fragmentation in post-pachytene spermatocytes and nucleolar reorganization in post-meiotic spermatids. Significant difference in the number and in the size of nucleoli between spermatogonia and round spermatids, as well as differences in the nucleolar position within the nucleus were also observed. Ultrastructural analysis showed the CB assembling in the cytoplasm of primary spermatocytes and the nucleolar fragmentation occurring at the same time. In conclusion our results suggest that the CB may play important roles during the spermatogenesis process in mammals and that its origin may be related to the nucleolar cycle during the meiotic cell cycle.

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“…As expected, the detailed analysis of these embryos indicated defects in transcription. The embryo nucleolus is thus of maternal (oocyte) origin [9,10].…”

Section: Manipulating the Mammalian Oocyte Nucleolimentioning

confidence: 99%

Transplantation of nucleoli into human zygotes: not as simple as expected?

Fulka

Langerova

Loi

et al. 2011

J Assist Reprod Genet

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The nucleolus of the maternal gamete is essential for life

Cited by 20 publications

References 12 publications

Caspase‐2_L, caspase‐9, and caspase‐3 during in vitro maturation and fragmentation of the mouse oocyte

Caspase‐2_L, caspase‐9, and caspase‐3 during in vitro maturation and fragmentation of the mouse oocyte

Morphological Changes of Mammalian Nucleoli during Spermatogenesis and Their Possible Role in the Chromatoid Body Assembling

Transplantation of nucleoli into human zygotes: not as simple as expected?

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The nucleolus of the maternal gamete is essential for life

Cited by 20 publications

References 12 publications

Caspase‐2L, caspase‐9, and caspase‐3 during in vitro maturation and fragmentation of the mouse oocyte

Caspase‐2L, caspase‐9, and caspase‐3 during in vitro maturation and fragmentation of the mouse oocyte

Morphological Changes of Mammalian Nucleoli during Spermatogenesis and Their Possible Role in the Chromatoid Body Assembling

Transplantation of nucleoli into human zygotes: not as simple as expected?

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Product

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Caspase‐2_L, caspase‐9, and caspase‐3 during in vitro maturation and fragmentation of the mouse oocyte

Caspase‐2_L, caspase‐9, and caspase‐3 during in vitro maturation and fragmentation of the mouse oocyte