The nucleoplasmic reticulum: form and function

Malhas, Ashraf N.; Goulbourne, Chris N.; Vaux, David J.

doi:10.1016/j.tcb.2011.03.008

Cited by 219 publications

(267 citation statements)

References 134 publications

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“…The sequence shows that, during the process of chromatin condensation and apparent changes in the shape of the nucleus leading to formation of apoptotic bodies, acidic vesicles migrate into nuclear membrane invaginations. Such membraneous structures are known to penetrate deep into the nucleus (21). Although the preliminary image data presented here do not shed light on the role of acidic vesicles in chromatin degradation, they demonstrate that the use of QA as a lysosomal tracer may facilitate long-term visualization of such processes.…”

Section: Acidic Vesicles In Apoptosismentioning

confidence: 58%

Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long‐term tracking of acidic vesicles

2014

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Acidic vesicles can be imaged and tracked in live cells after staining with several low molecular weight fluorescent probes, or with fluorescently labeled proteins. Three fluorescent dyes, acridine orange, LysoTracker Red DND-99, and quinacrine, were evaluated as acidic vesicle tracers for confocal fluorescence imaging and quantitative analysis. The stability of fluorescent signals, achievable image contrast, and phototoxicity were taken into consideration. The three tested tracers exhibit different advantages and pose different problems in imaging experiments. Acridine orange makes it possible to distinguish acidic vesicles with different internal pH but is fairly phototoxic and can cause spectacular bursts of the dye-loaded vesicles. LysoTracker Red is less phototoxic but its rapid photobleaching limits the range of useful applications considerably. We demonstrate that quinacrine is most suitable for long-term imaging when a high number of frames is required. This capacity made it possible to trace acidic vesicles for several hours, during a process of drug-induced apoptosis. An ability to record the behavior of acidic vesicles over such long periods opens a possibility to study processes like autophagy or long-term effects of drugs on endocytosis and exocytosis. V C 2014International Society for Advancement of Cytometry

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Section: Acidic Vesicles In Apoptosismentioning

confidence: 58%

Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long‐term tracking of acidic vesicles

2014

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“…After farnesylated prelamin A accumulates the nucleus becomes dysmorphic with a highly convoluted nuclear envelope with many nuclear invaginations. However, the relationship between these morphological changes and the increasingly well-recognised nuclear invaginations observed in different cell types and during various stages of cellular development remains obscure (Fricker et al, 1997a;Fricker et al, 1997b;Malhas et al, 2011). Such structures are believed to increase the interface between nucleoplasm and cytoplasm to improve communication between the two environments as well as add structural support.…”

Section: Introductionmentioning

confidence: 99%

The induction of a nucleoplasmic reticulum by prelamin A accumulation requires CTP:phosphocholine cytidylyltransferase-α

Goulbourne

Malhas

Vaux

2011

Journal of Cell Science

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SummaryFarnesylated prelamin A accumulates when the final endoproteolytic maturation of the protein fails to occur and causes a dysmorphic nuclear phenotype; however, the morphology and mechanisms of biogenesis of these changes remain unclear. We show here that acute prelamin A accumulation after reduction in the activity of the ZMPSTE24 endoprotease by short interfering RNA knockdown, results in the generation of a complex nucleoplasmic reticulum that depends for its formation on the enzyme CTP:phosphocholinecytidylyltransferase-a (CCT-a, also known as choline-phosphate cytidylyltransferase A). This structure can form during interphase, confirming that it is independent of mitosis and therefore not a consequence of disordered nuclear envelope assembly. Serial-section dual-axis electron tomography reveals that these invaginations can take two forms: one in which the inner nuclear membrane infolds alone with an inter membrane space interior, and the other in which an invagination of both nuclear membranes occurs, enclosing a cytoplasmic core. Both types of invagination can co-exist in one nucleus and both are frequently studded with nuclear pore complexes (NPC), which reduces NPC abundance on the nuclear surface.

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“…Intranuclear phosphorylated inositol phospholipids (PIPs) have been known for years and their roles in chromatin remodeling is a subject of active research (Shah et al, 2013). Moreover, many (and maybe most) cell types have a nucleoplasmic reticulum (NR) (Malhas et al, 2011). The NR, derived from the nuclear envelope, may contain a lumen continuous with cytoplasm as well as one with the intermembrane space between the inner and outer nuclear envelope membranes.…”

Section: Associations With the Nucleusmentioning

confidence: 99%

Molecular Mechanisms and Physiological Significance of Organelle Interactions and Cooperation

2017

Frontiers Research Topics

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The nucleoplasmic reticulum: form and function

Cited by 219 publications

References 134 publications

Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long‐term tracking of acidic vesicles

Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long‐term tracking of acidic vesicles

The induction of a nucleoplasmic reticulum by prelamin A accumulation requires CTP:phosphocholine cytidylyltransferase-α

Molecular Mechanisms and Physiological Significance of Organelle Interactions and Cooperation

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