The nucleotide sequence of a recombinant tomato yellow leaf curl virus strain frequently detected in Sicily isolated from tomato plants carrying the Ty-1 resistance gene

Panno, Stefano; Caruso, A.; Davino, Salvatore

doi:10.1007/s00705-017-3674-9

Cited by 31 publications

(25 citation statements)

References 7 publications

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“…The production of infectious DF-640 clones is now underway, and they will be used to verify this hypothesis. Nevertheless, it is well documented that the increase in the acreage of cultivars harboring resistance genes such as Ty -1 can result in strong selection forces towards more aggressive viral isolates, accelerating the change in the composition of the viral population and potentially culminating with the loss of effectivity of the source of resistance/tolerance [ 71 , 72 , 73 , 74 , 75 ]. Studies have also reported the “breakdown” of the resistance mediated by the Ty -2 gene caused by a strain of Tomato yellow leaf curl virus (TYLCV) [ 76 ] and by a strain of the Tomato leaf curl Bangalore virus (ToLCBV) in India [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene

Reis

Fonseca

Ribeiro

et al. 2020

Viruses

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A complex of begomoviruses (Geminiviridae) can cause severe tomato yield losses in the neotropics. Here, next-generation sequencing was employed for large-scale assessment of single-stranded (ss)DNA virus diversity in tomatoes either harboring or lacking the large-spectrum begomovirus tolerance Ty-1 gene. Individual leaf samples exhibiting begomovirus-like symptoms (n = 107) were field-collected, circular DNA-enriched, subdivided into pools (with and without Ty-1), and Illumina-sequenced. Virus-specific PCR and Sanger dideoxy sequencing validations confirmed 15 distinct ssDNA virus/subviral agents (occurring mainly in mixed infections), which highlight the potential drawbacks of employing virus-specific resistance in tomato breeding. More viruses (14 versus 6 species) were observed in tomatoes without the Ty-1 gene. A gemycircularvirus (Genomoviridae), a new alpha-satellite, and two novel Begomovirus species were identified exclusively in samples without the Ty-1 gene. A novel begomovirus was found only in the Ty-1 pool, being the only species associated with severe symptoms in Ty-1 plants in our survey. Our work is the first step towards the elucidation of the potential begomovirus adaptation to Ty-1 and its specific filtering effects on a subset of ssDNA viral/subviral agents.

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Section: Discussionmentioning

confidence: 99%

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene

Reis

Fonseca

Ribeiro

et al. 2020

Viruses

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“…Recently several articles have been published on Ty‐1 resistance‐breaking strains of TYLCV in cultivations of Ty‐1 ‐bearing tomato. Samples of Ty‐1 plants showing TYLCV‐like symptoms collected in Morocco, Italy and Spain revealed the presence of viruses derived from a recombination event between TYLCV and TYLCSV in which a noncoding region between the origin of replication and the start of the coding region of V2 were exchanged (Belabess et al , ; Granier et al , ; Panno et al , ; Torre et al , ). In Morocco, this recombinant replaced both parental strains, but also under laboratory conditions this recombinant was positively selected in Ty‐1 ‐bearing plants (Belabess et al , , ).…”

Section: Discussionmentioning

confidence: 99%

Ty‐1, a universal resistance gene against geminiviruses that is compromised by co‐replication of a betasatellite

Voorburg

Yan

Bergua-Vidal

et al. 2019

Molecular Plant Pathology

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Tomato yellow leaf curl virus (TYLCV), a begomovirus, causes large yield losses and breeding for resistance is an effective way to combat this viral disease. The resistance gene Ty-1 codes for an RNA-dependent RNA polymerase and has recently been shown to enhance transcriptional gene silencing of TYLCV. Whereas Ty-1 was earlier shown to also confer resistance to a bipartite begomovirus, here it is shown that Ty-1 is probably generic to all geminiviruses. A tomato Ty-1 introgression line, but also stable transformants of susceptible tomato cv. Moneymaker and Nicotiana benthamiana (N. benthamiana) expressing the Ty-1 gene, exhibited resistance to begomoviruses as well as to the distinct, leafhopper-transmitted beet curly top virus, a curtovirus. Stable Ty-1 transformants of N. benthamiana and tomato showed fewer symptoms and reduced viral titres on infection compared to wild-type plants. TYLCV infections in wild-type N. benthamiana plants in the additional presence of a betasatellite led to increased symptom severity and a consistent, slightly lowered virus titre relative to the high averaged levels seen in the absence of the betasatellite. On the contrary, in Ty-1 transformed N. benthamiana viral titres increased in the presence of the betasatellite. The same was observed when these Ty-1-encoding plants were challenged with TYLCV and a potato virus X construct expressing the RNA interference suppressor protein βC1 encoded by the betasatellite. The resistance spectrum of Ty-1 and the durability of the resistance are discussed in light of antiviral RNA interference and viral counter defence strategies.

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“…Among monopartite begomoviruses, the Tomato yellow leaf curl virus (TYLCV) species is an example of an emergent begomovirus that seriously impacts tomato ( Solanum lycopersicum ) production, it causes the tomato yellow leaf curl disease (TYLCD) epidemics throughout the tropical and sub-tropical regions of the world [28]. A large body of information is available about the genetic diversity, biology and worldwide spread patterns of TYLCV [7,29,30,31,32,33,34,35]. In contrast, much less information is available for other begomoviruses also associated with TYLCD epidemics.…”

Section: Introductionmentioning

confidence: 99%

Tomato Yellow Leaf Curl Sardinia Virus, a Begomovirus Species Evolving by Mutation and Recombination: A Challenge for Virus Control

Díaz-Pendón

Sánchez-Campos

Fortes

et al. 2019

Viruses

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The tomato leaf curl disease (TYLCD) is associated with infections of several species of begomoviruses (genus Begomovirus, family Geminiviridae) and causes severe damage to tomatoes throughout tropical and sub-tropical regions of the world. Among others, the Tomato yellow leaf curl Sardinia virus (TYLCSV) species causes damage in the Mediterranean Basin since early outbreaks occurred. Nevertheless, scarce information is available about the diversity of TYLCSV. Here, we study this aspect based on the sequence information accessible in databases. Isolates of two taxonomically differentiated TYLCSV strains can be found in natural epidemics. Their evolution is mostly associated with mutation combined with selection and random genetic drift and also with inter-species recombination which is frequent in begomoviruses. Moreover, a novel putative inter-strain recombinant is reported. Although no significantly new biological behaviour was observed for this latter recombinant, its occurrence supports that as shown for other related begomoviruses, recombination continues to play a central role in the evolution of TYLCD-associated viruses and the dynamism of their populations. The confrontation of resistant tomatoes with isolates of different TYLCD-associated viruses including the novel recombinant demonstrates the existence of a variable virus x plant genotype interaction. This has already been observed for other TYLCD-associated viruses and is a challenge for the control of their impact on tomato production.

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The nucleotide sequence of a recombinant tomato yellow leaf curl virus strain frequently detected in Sicily isolated from tomato plants carrying the Ty-1 resistance gene

Cited by 31 publications

References 7 publications

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene

Ty‐1, a universal resistance gene against geminiviruses that is compromised by co‐replication of a betasatellite

Tomato Yellow Leaf Curl Sardinia Virus, a Begomovirus Species Evolving by Mutation and Recombination: A Challenge for Virus Control

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