The nucleotide sequence of RNA 2 of barley yellow mosaic virus

Davidson, Andrew D.; Pröls, Markus; Schell, Jeff; Steinbiß, Hans‐Henning

doi:10.1099/0022-1317-72-4-989

Cited by 52 publications

(32 citation statements)

References 19 publications

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“…The extensive sequence similarities, despite differences in genome organization, between the proteins encoded by potyviruses and BaYMV have been described (8,9,12). Inspection of the local similarity plots revealed one of the conserved motifs of the RNA polymerases of the positive-strand RNA viruses (designated motif IV in Fig.…”

Section: Resultsmentioning

confidence: 94%

Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses.

Koonin

Choi

Nuss

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

168

119

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Computer-assisted analysis of the putative polypeptide products encoded by the two open reading frames present in a large virus-like double-stranded RNA, L-dsRNA, associated with hypovirulence of the chestnut blight fungus, Cryphonectria parasitica, revealed five distinct domains with significant sequence similarity to previously described conserved domains within plant potyvirus-encoded polyproteins.These included the putative RNA-dependent RNA polymerase, RNA helicase, two papain-like cysteine proteases related to the potyvirus helper-component protease, and a cysteine-rich domain of unknown function similar to the N-terminal portion of the potyvirus helper-component protein. Phylogenetic trees derived from the alignment of the polymerase domains of L-dsRNA, a subset of positive-stranded RNA viruses, and double-stranded RNA viruses, using three independent algorithms, suggested that the hypovirulence-associated dsRNA and potyvirus genomes share a common ancestry. However, comparison of the organization of the conserved domains within the encoded polyproteins of the respective viruses indicated that the proposed subsequent evolution involved extensive genome rearrangement.The phenomenon of transmissible hypovirulence represents a natural form of biological control in which the virulence of Cryphonectria parasitica, the chestnut blight fungus, is modulated by the presence of virus-like genetic elements composed of double-stranded RNA (dsRNA) (reviewed in refs. 1 to 4). Efforts to understand the molecular mechanisms responsible for transmissible hypovirulence have provided an emerging view of the structural and functional properties of hypovirulence-associated dsRNA genetic elements (reviewed in ref. 5). The largest dsRNA present in C. parasitica strain Ep713, L-dsRNA (12,712 base pairs), was shown recently to contain two contiguous coding domains, designated open reading frame (ORF) A and ORF B, consisting of 622 and 3165 codons, respectively (6). Both ORFs encode polyproteins that undergo autocatalytic processing during or immediately after translation (6, 7). On the basis of the similarity of the L-dsRNA genetic organization and expression strategy to those of several viral genomes, it was suggested that L-dsRNA should be considered the equivalent of a viral genome or replicative form and the descriptive term hypovirulence-associated virus (HAV) was proposed (6).Similarities between one of the HAV-encoded proteases, p29, and the potyvirus-encoded protease, HC-Pro, were noted previously (7). In addition, computer-assisted analysis of the C-terminal portion of the ORF B-encoded polyprotein revealed a domain that was clearly related to the RNA helicase of potyviruses (6). We now extend these observations by demonstrating that three additional domains are conserved between the gene products of HAV and those of potyviruses, including the putative RNA polymerase. A detailed analysis of the sequence and organization of the conserved domains within the HAV-encoded and potyvirusencoded polyproteins suggests tha...

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Section: Resultsmentioning

confidence: 94%

Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses.

Koonin

Choi

Nuss

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

168

119

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“…However, studies on bymoviruses have so far been focussed on the sequence analysis of the viral genomes and electron microscopy of infected plants (Huth et al, 1984;Kashiwazaki et al, 1990;Davidson et al, 1991;Peerenboom et al, 1992;Schenk et al, 1993), and nothing is known about the nucleic acid-binding characteristics of any bymoviral CP.…”

mentioning

confidence: 99%

“…BaYMV is transmitted by the soil-borne fungus Polymyxa graminis and is classified in the genus Bymovirus of the Potyviridae, the largest family of plant RNA-viruses (Murphy et al, 1995). Its bipartite, plus-strand RNA genome codes for two large polyproteins, which are processed by virus-encoded proteases (Kashiwazaki et al, 1989;Davidson et al, 1991).…”

mentioning

confidence: 99%

Cooperative binding to nucleic acids by barley yellow mosaic bymovirus coat protein and characterization of a nucleic acid-binding domain

Reichel

Maas²,

Schulze³

et al. 1996

Journal of General Virology

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The capacity of several coat protein (CP) mutants of a German isolate of barley yellow mosaic bymovirus (BaYMV) to bind to nucleic acids was studied in vitro. Recombinant CP, produced by overexpression in Escherichia eoli, was purified from inclusion bodies and subsequently renatured. Binding to single-stranded (ss) RNA and ssDNA oligonucleotides was found to be cooperative and sequence non-specific. By deletion mutagenesis, several truncated CP derivatives were created and their nucleic acid-binding capacity was investigated in order to define a protein domain responsible for RNA-and DNA-binding. The nucleic acid-binding domain consists of a core which was located to an internal 23 amino acid peptide (aa 125-147) and an adjacent domain (aa 148-184) which stimulates binding.

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“…Barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) are two members of this genus that cause, often in mixed infection, the economically important yellow mosaic disease of winter barley (Hordeum vulgare) in Europe and Asia Huth & Adams, 1990 ;Hariri et al, 1990). Bymovirus research initially focused on the type species, BaYMV, whose entire genomic sequence was published for Japanese and German isolates (Kashiwazaki et al, 1990(Kashiwazaki et al, , 1991Davidson et al, 1991 ;Peerenboom et al, 1992). Since then, progress on the study of its molecular biology has been hampered by the lack of infectious clones to enable reverse genetics.…”

mentioning

confidence: 99%

35S promoter-driven cDNAs of barley mild mosaic virus RNA1 and RNA2 are infectious on barley plants.

Meyer

Dessens²

1997

Journal of General Virology

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The nucleotide sequence of RNA 2 of barley yellow mosaic virus

Cited by 52 publications

References 19 publications

Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses.

Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses.

Cooperative binding to nucleic acids by barley yellow mosaic bymovirus coat protein and characterization of a nucleic acid-binding domain

35S promoter-driven cDNAs of barley mild mosaic virus RNA1 and RNA2 are infectious on barley plants.

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