The Nucleotide Sequence of the Equine Herpesvirus 4 Thymidine Kinase Gene

Nicolson, Lesley; Cullinane, Ann; Onions, David

doi:10.1099/0022-1317-71-8-1801

Cited by 22 publications

(7 citation statements)

References 28 publications

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“…This region of the sialyltransferases has recently been shown to be part of the binding site for CMP-sialic acids by sitedirected mutagenesis [30]. A sequence of five amino acids which were similar or identical to this region was also found in the nucleotide-binding region of the thymidine kinase from herpes viruses [31]. The structural similarity of thymidine and cytidine supports the hypothesis that the presented sequence of the hydroxylase is involved in pyridine nucleotide binding (see Fig.…”

Section: Discussionsupporting

confidence: 64%

CMP‐N‐acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron‐sulphur protein to be described in Eukarya

et al. 1996

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Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-Nacetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known to be characteristic for a Rieske ironsulphur centre (2Fe-2S), the g-values being 1.78, 1.91 and 2.01, respectively. An analysis of the primary structure of the hydroxylase led to the identification of an amino acid sequence, known to be characteristic for Rieske proteins. Furthermore, possible binding sites for cytochrome bs, the substrate CMPNeu5Ac and a mononuclear iron centre were also identified.

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Section: Discussionsupporting

confidence: 64%

CMP‐N‐acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron‐sulphur protein to be described in Eukarya

et al. 1996

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“…The complete nucleotide sequence (150,223 nucleotides) of the EHV 1 genome has become available [41]. The nucleotide sequence of several genes in the EHV4 genome has been determined including genes in the UL region encoding glycoprotein gB, gC, and gH and thymidine kinase [25][26][27]33]. In the US region the sequence of the Barn HI L restriction fragment, which contains parts of US region extending into the terminal repeat, has also been determined [7].…”

Section: T44mentioning

confidence: 99%

Analysis of the nucleotide sequence of five genes at the left end of the unique short region of the equine herpesvirus 4 genome

Nagesha

Crabb

Studdert

1993

Archives of Virology

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Eco RI fragment G of equine herpesvirus 4 strain 405/76 (EHV 4.405/76) is located at the left end of the unique short region close to or extending into the internal repeat region of the prototypic arrangement of the genome. The nucleotide sequence of two subclones designated HS and G 19, contiguous within Eco RI fragment G, was determined for each strand by obtaining a nested set of deletion clones of these double-stranded DNA plasmids. Analysis of the nucleotide sequence revealed that the two subclones contain 5449 base pairs with four complete open reading frames (ORFs) and part of a fifth ORF. Comparison of the predicted amino acid sequences of these reading frames showed that they correspond to ORFs 67, 68, 69, 70, and 71 of equine herpesvirus type 1 (EHV 1) [41], of which ORFs 68, 69, and 70 are homologous to human herpes simplex virus (HSV) genes in the unique short (US) region, i.e., US 2, US 3, and US 4. ORF 67' of EHV 4 and ORF 67 of EHV 1 are homologous (65.7%) but these genes have no homologue in HSV 1.

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“…The nucleotide sequences of the TK genes of a variety of herpesviruses, including EHV‐1 and EHV‐4, have been reported ( Robertson and Whalley, 1988, Nicolson et al, 1990; Telford et al, 1992; Telford et al, 1998). Alignment comparison of the TK amino acid sequences of EHV1 and EHV4 indicates 89% identity between them.…”

Section: Methodsmentioning

confidence: 99%

“…Young horses are usually infected by EHV‐1 and ‐4 in the first year of life, and both can be present simultaneously in seronegative animals, arising from the transient nature of the antibody response to these viruses ( Allen and Bryans, 1986). The heterogeneity between EHV‐1 and EHV‐4 has been demonstrated through restriction endonuclease analysis ( Sabine et al, 1981; Studdert et al, 1981) and recent nucleotide sequence alignment of their genome estimated a genetic identity that ranges from 65 to 85% ( Nicolson and Onions, 1990; Nicolson et al, 1990; Telford et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Development of a Differential Multiplex PCR Assay for Equine Herpesvirus 1 and 4 as a Diagnostic Tool

Carvalho

Passos

Martins

2000

Journal of Veterinary Medicine, Series B

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In this study, a multiplex polymerase chain reaction (PCR) procedure was developed for differentiation of strains and field isolates of equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4). Specific oli-gonucleotide primers were combined to amplify the thymidine kinase (TK) gene region of EHV-1 and EHV-4, which would yield fragments of different lengths for each virus in the same amplification reaction. The specificity of the largest PCR amplicon for EHV-4 was confirmed by restriction digestion with HindIII. The multiplex PCR proved to be a fast and sensitive method for typing EHV-1 and EHV-4 isolates and for detection and differentiation of both viruses in field samples in which infectious virus is no longer available. The sensitivity was improved by combining cycling optimization and visualization of PCR products in ethidium bromide and silver-stained acrylamide gels.

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The Nucleotide Sequence of the Equine Herpesvirus 4 Thymidine Kinase Gene

Cited by 22 publications

References 28 publications

CMP‐N‐acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron‐sulphur protein to be described in Eukarya

CMP‐N‐acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron‐sulphur protein to be described in Eukarya

Analysis of the nucleotide sequence of five genes at the left end of the unique short region of the equine herpesvirus 4 genome

Development of a Differential Multiplex PCR Assay for Equine Herpesvirus 1 and 4 as a Diagnostic Tool

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