The nucleotide sequence of the major glutamate transfer RNA from Schizosaccharomyces pombe

Abstract

The nucleotide sequence of glutamate tRNA1 from Schizosaccharomyces pombe was determined to be pU-C-C-G-U-U-G-U-m1G-G-U-C-C-A-A-C-G-G-C-D-A-G-G-A-U-U-C-G-U-C-G-C-U-U-U*-C-A-C-C-G-A-C-G-G-G-A-G-m5C-G-G-G-G-T-psi-C-G-A-C-U-C-C-C-C-G-C-A-A-C-G-G-A-G-C-C-AOH. The sequence differs markedly from that of S. cerevisiae tRNAGlu. S. pombe glutamate tRNA1 can be aminoacylated by the homologous glutaminyl-tRNA synthetase as well as by the corresponding enzyme from S. cerevisiae.

5-Methyl-2-thiouridine in the tRNA of Candida tropicalis and its localization in lysine tRNA

“…Since both the thionucleotides were found in the RNase A-resistant fragment (see above), the results established that both of the thionucleotides were associated with Up only. The mobilities of the dinucleotides on DEAE-cellulose and Whatman 3 MM paper were in agreement for a dinucleotide of the type UJ*pUp or UpU*p. Observations similar to this have been made by other research workers, who encountered a RNase-resistant dinucleotide, mcm5s2Up followed by uri- (10,11,13,17). The present resu the presence of mcm5s2UpUp in the RNase tropicalis tRNA.…”

The nucleotide sequence of a glutamate tRNA from rat liver

“…Its nucleotide sequence is remarkably similar to that of tRNAGlu from 4 Drosophila melanogaster (12); both tRNAs are 75 nucleotides long, and only 10 out of 75 nucleotides in the two tRNAs are completely different. The homology to the glutamate tRNAs from yeasts is considerably less; 21 out of the 75 nucleotides in S. pombe tRNAGlu (13) and 23 out of the 75 nucleotides in S. cerevisiae tRNAGlu (14) are different. The divergence from the glutamate tRNAs of bacteria is even more striking; the tRNAGlu 2 from E. coli (15) is 76 nucleotides long, of which 31 are different from the rat liver tRNAGlu including an additional nucleotide in the D loop; finally, the tRNAG'u from H. volcanii (16) is 78 nucleotides long and differs in 34 nucleotides, including three extra nucleotides in the D loop.…”

An early step in wobble uridine tRNA modification requires the Elongator complex

“…Strains with tagged Elp proteins were killer toxin sensitive, confirming the functionality of the tagged proteins. (Wong et al 1979) from the sin3 + strain contained mcm 5 s 2 U, whereas it was reduced in the sin3-193 (5% of wild type) and not detectable in the sin3ϻKanMX6 derivative (data not shown). Furthermore, analysis of the ochre suppressor tRNA Ser revealed the presence of mcm 5 U in the ade7-413 sup3-18 strain, but not in the sin3-193 and sin3ϻKanMX6 derivatives or the wild-type ade7-413 strain (data not shown).…”