The Nucleus of HeLa Cell Contains Tubular Structures for Ca2+Signalling

Lui, Pauline Po Yee; Kong, Siu Kai; Kwok, Tim Tak; Lee, C.Y.

doi:10.1006/bbrc.1998.8649

Cited by 50 publications

(40 citation statements)

References 25 publications

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“…The first consists of an infolding of both the INM and ONM, with a cytoplasmic core that often contains nuclear pore complexes. The purpose of the double membrane invagination has been studied and it is proposed to act as a conduit for localised calcium release within the nucleus to regulate transcription of genes controlled by calcium (Bkaily et al, 2009;Bootman et al, 2009;Chamero et al, 2008;ColladoHilly et al, 2010;Guatimosim et al, 2008;Lui et al, 1998;Marius et al, 2006;Nalaskowski et al, 2011). This might help the cell to adapt during the pathological accumulation of prelamin A.…”

Section: Discussionmentioning

confidence: 99%

The induction of a nucleoplasmic reticulum by prelamin A accumulation requires CTP:phosphocholine cytidylyltransferase-α

Goulbourne

Malhas

Vaux

2011

Journal of Cell Science

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SummaryFarnesylated prelamin A accumulates when the final endoproteolytic maturation of the protein fails to occur and causes a dysmorphic nuclear phenotype; however, the morphology and mechanisms of biogenesis of these changes remain unclear. We show here that acute prelamin A accumulation after reduction in the activity of the ZMPSTE24 endoprotease by short interfering RNA knockdown, results in the generation of a complex nucleoplasmic reticulum that depends for its formation on the enzyme CTP:phosphocholinecytidylyltransferase-a (CCT-a, also known as choline-phosphate cytidylyltransferase A). This structure can form during interphase, confirming that it is independent of mitosis and therefore not a consequence of disordered nuclear envelope assembly. Serial-section dual-axis electron tomography reveals that these invaginations can take two forms: one in which the inner nuclear membrane infolds alone with an inter membrane space interior, and the other in which an invagination of both nuclear membranes occurs, enclosing a cytoplasmic core. Both types of invagination can co-exist in one nucleus and both are frequently studded with nuclear pore complexes (NPC), which reduces NPC abundance on the nuclear surface.

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Section: Discussionmentioning

confidence: 99%

The induction of a nucleoplasmic reticulum by prelamin A accumulation requires CTP:phosphocholine cytidylyltransferase-α

Goulbourne

Malhas

Vaux

2011

Journal of Cell Science

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“…The presence of tubular structures inside the nucleus was described in the C6 glioma cell line and in HeLa cells. These structures apparently correspond to extrusions of the nuclear envelope, enriched in IP 3 R (Lui et al, 1998;Lui et al, 2003). In isolated nuclei from Xenopus oocytes, expression of lamin B1 and B2, was demonstrated to induce formation of intranuclear membranes that resemble ER cisternae (Ralle et al, 2004).…”

Section: Camentioning

confidence: 99%

“…However, it has been demonstrated both in oocytes and in HeLa cells that it is possible to induce an IP 3 -dependent rise of nuclear Ca 2+ , independently of the cytoplasm (Lui et al, 1998;Santella et al, 1998). The participation of IP 3 Rs in regulating nuclear Ca 2+ in skeletal muscle has not yet been described.…”

Section: Camentioning

confidence: 99%

Nuclear inositol 1,4,5-trisphosphate receptors regulate local Ca2+ transients and modulate cAMP response element binding protein phosphorylation

Cárdenas

Liberona

Molgó

et al. 2005

Journal of Cell Science

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Several lines of evidence indicate that increases in nuclear Ca2+ have specific biological effects that differ from those of cytosolic Ca2+, suggesting that they occur independently. The mechanisms involved in controlling nuclear Ca2+ signaling are both controversial and still poorly understood. Using hypotonic shock combined with mechanical disruption, we obtained and characterized a fraction of purified nuclei from cultured rat skeletal myotubes. Both immunoblot studies and radiolabeled inositol 1,4,5-trisphosphate [IP3] binding revealed an important concentration of IP3 receptors in the nuclear fraction. Immunofluorescence and immunoelectron microscopy studies localized type-1 and type-3 IP3 receptors in the nucleus with type-1 receptors preferentially localized in the inner nuclear membrane. Type-2 IP3 receptor was confined to the sarcoplasmic reticulum. Isolated nuclei responded to IP3 with rapid and transient Ca2+ concentration elevations, which were inhibited by known blockers of IP3 signals. Similar results were obtained with isolated nuclei from the 1B5 cell line, which does not express ryanodine receptors but releases nuclear Ca2+ in an IP3-dependent manner. Nuclear Ca2+ increases triggered by IP3 evoked phosphorylation of cAMP response element binding protein with kinetics compatible with sequential activation. These results support the idea that Ca2+ signals, mediated by nuclear IP3 receptors in muscle cells, are part of a distinct Ca2+ release component that originates in the nucleus and probably participates in gene regulation mediated by cAMP response element binding protein.

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“…Electron microscopy has shown that these nuclear invaginations can penetrate deep into the nucleus and confirmed the presence of the double membrane of the nuclear envelope (Ellenberg et al, 1997;Fricker et al, 1997;Clubb and Locke, 1998). However, revelation of the full nature of nuclear invaginations in living animal cells has required confocal microscopy of fluorescent dyes (Fricker et al, 1997;Lui et al, 1998aLui et al, , 1998b or green fluorescent protein (GFP) fusion proteins targeted to the nuclear envelope (Ellenberg et al, 1997;Broers et al, 1999). The number and nature of invaginations vary from cell type to cell type, ranging from simple invaginations to intricate branched structures that can penetrate into and through the nucleus.…”

Section: Introductionmentioning

confidence: 99%

“…Invaginations often associate with nucleoli (Fricker et al, 1997) and appear to be stable (Ellenberg et al, 1997;Broers et al, 1999). Nuclear grooves and invaginations substantially increase the surface area of the nucleus and have been suggested to function in signaling from the cytoplasm to the nucleus (Lui et al, 1998a(Lui et al, , 1998b or to coordinate transport processes between the nucleus and the cytoplasm (Fricker et al, 1997). However, whether any relationship exists between the distribution of nuclear invaginations and chromosome domains within the animal nucleus remains to be determined.…”

Section: Introductionmentioning

confidence: 99%

Plant Nuclei Can Contain Extensive Grooves and Invaginations [W]

et al. 2000

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Plant cells can exhibit highly complex nuclear organization. Through dye-labeling experiments in untransformed onion epidermal and tobacco culture cells and through the expression of green fluorescent protein targeted to either the nucleus or the lumen of the endoplasmic reticulum/nuclear envelope in these cells, we have visualized deep grooves and invaginations into the large nuclei of these cells. In onion, these structures, which are similar to invaginations seen in some animal cells, form tubular or planelike infoldings of the nuclear envelope. Both grooves and invaginations are stable structures, and both have cytoplasmic cores containing actin bundles that can support cytoplasmic streaming. In dividing tobacco cells, invaginations seem to form during cell division, possibly from strands of the endoplasmic reticulum trapped in the reforming nucleus. The substantial increase in nuclear surface area resulting from these grooves and invaginations, their apparent preference for association with nucleoli, and the presence in them of actin bundles that support vesicle motility suggest that the structures might function both in mRNA export from the nucleus and in protein import from the cytoplasm to the nucleus. INTRODUCTIONAlthough cellular function often requires maximization of surface area relative to volume, notably in organelles such as the endoplasmic reticulum (ER) and Golgi apparatus, traditional representations of the nucleus depict a rounded structure with little internal organization. Recently, however, the nuclei of animal cells have been found to show considerable spatial and structural organization at the chromosomal level. The discrete and comparatively stable territories that chromosomes occupy within the nucleus are separated by interchromosomal domains through which transcribed RNA and other macromolecules can diffuse (reviewed in Lamond and Earnshaw, 1998). Animal nuclei also sometimes deviate from the characteristic rounded shape. Deviations include a folded, grooved, or notched surface (Majno et al., 1969) and tubular invaginations. Electron microscopy has shown that these nuclear invaginations can penetrate deep into the nucleus and confirmed the presence of the double membrane of the nuclear envelope (Ellenberg et al., 1997;Fricker et al., 1997;Clubb and Locke, 1998). However, revelation of the full nature of nuclear invaginations in living animal cells has required confocal microscopy of fluorescent dyes (Fricker et al., 1997;Lui et al., 1998aLui et al., , 1998b or green fluorescent protein (GFP) fusion proteins targeted to the nuclear envelope (Ellenberg et al., 1997;Broers et al., 1999). The number and nature of invaginations vary from cell type to cell type, ranging from simple invaginations to intricate branched structures that can penetrate into and through the nucleus. Invaginations often associate with nucleoli (Fricker et al., 1997) and appear to be stable (Ellenberg et al., 1997;Broers et al., 1999). Nuclear grooves and invaginations substantially increase the surface area of the ...

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The Nucleus of HeLa Cell Contains Tubular Structures for Ca2+Signalling

Cited by 50 publications

References 25 publications

The induction of a nucleoplasmic reticulum by prelamin A accumulation requires CTP:phosphocholine cytidylyltransferase-α

The induction of a nucleoplasmic reticulum by prelamin A accumulation requires CTP:phosphocholine cytidylyltransferase-α

Nuclear inositol 1,4,5-trisphosphate receptors regulate local Ca2+ transients and modulate cAMP response element binding protein phosphorylation

Plant Nuclei Can Contain Extensive Grooves and Invaginations [W]

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