The number of nucleoli in benign and malignant thyroid lesions: a useful diagnostic sign in cytological preparations*

Montironi, R; Braccischi, A.; Scarpelli, Marina; Sisti, S.; Matera, Giovanni; Mariuzzi, Gian Mario; Alberti, R; Collan, Yrjö

doi:10.1111/j.1365-2303.1990.tb00341.x

Cited by 10 publications

(3 citation statements)

References 14 publications

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“…The available evidences indicate that the nucleolar changes in morphology or function are the consequence of increased demand for ribosome biogenesis. PTC has several significant alterations which are observed both in the fine structure and in the number of nucleoli [ 17 , 18 ]. These studies raise the possibility of the interference of a ribosome biogenesis thus inducing PTC pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

RRS1 gene expression involved in the progression of papillary thyroid carcinoma

Chen

Jin

et al. 2018

Cancer Cell Int

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BackgroundPapillary thyroid carcinoma (PTC) is one of the most frequent malignancies of the endocrine system, whose mechanisms of pathogenesis, progression and prognosis are still far from being clearly elucidated. Despite an increasing body of evidences highlights ribosome biogenesis regulator homolog (RRS1) as a ribosome biogenesis protein in yeast and plants, little is known about human RRS1 function.MethodsProliferation, cell cycle and apoptosis of PTC cells were assessed following the knockdown of RRS1 expression though MTT, colony formation assay, and flow cytometry. Then, transcriptome profiling was conducted to explore pathway changes after RRS1 silencing in PTC cells. Receiver operating characteristic curve and Youden’s index were performed in twenty-four thyroid carcinoma samples to assess their potential clinical diagnostic value.ResultsFirstly, we found that silencing RRS1 significantly reduced cell proliferation, inhibited cell cycle, and promoted apoptosis in PTC cell line. The result also showed that knock-down of RRS1 could up-regulate genes involving apoptosis and metabolism, while, down-regulate genes relative to cell proliferation and blood vessel development. Notably, the present study confirmed the diagnostic value of RRS1 for thyroid carcinoma in both children and adults.ConclusionsIn conclusion, these data afford a comprehensive view of a novel function of human RRS1 by promoting cell proliferation and could be a potential indicator for papillary thyroid carcinoma.Electronic supplementary materialThe online version of this article (10.1186/s12935-018-0519-x) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

RRS1 gene expression involved in the progression of papillary thyroid carcinoma

Chen

Jin

et al. 2018

Cancer Cell Int

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“…Montironi and coworkers, in various studies, [14][15][16][17] had shown the ability of nucleolar counts to differentiate follicular adenomas from follicular carcinomas. In our study all the nucleolar parameters studied showed an overlap (including nuclear margination).…”

Section: Discussionmentioning

confidence: 99%

“…In spite of the profusion of work available, only marginal success has been achieved in separating them cytologically. Parameters used for this purpose include morphologic features, such as cellular patterns, [9][10][11]18 morphometric criteria like nuclear diameter, 1,2,12 and number and margination of nucleoli [14][15][16][17] and flow cytometry, 3,5,6 either singly or in combination. The sensi-tivity of these results has varied from 40% 19 to 75%.…”

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confidence: 99%

Follicular Neoplasms of the Thyroid

Deshpande

Kapila²,

Sai³

et al. 1997

Acta Cytologica

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Quantitative analysis of nucleolar margination in diagnostic cytopathology

Montironi

Scarpelli

Braccischi³

et al. 1991

Vichows Archiv A Pathol Anat

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The diagnostic value of nucleolar margination, defined as the percentage of nucleoli touching the nuclear membrane, was investigated in 359 cytological preparations of benign and malignant lesions of the thyroid, breast, prostate and central nervous system. Premalignant lesions of the uterine cervix and non-invasive papillary carcinomas of the bladder were also examined. It was observed that the percentages in benign lesions were, in general, lower than in the malignant and that the values increased progressively with increasing grade in the cervix and bladder. When the overlap index was calculated, this gave exact information on the usefulness of nucleolar margination in distinguishing benign from malignant lesions, particularly in the prostate and thyroid and, to a lesser extent, in the breast and central nervous system. As for lesions of different grades, the calculation of the index allowed the identification of two subgroups, one corresponding to low grades (mild cervical dysplasia or urothelial papillary carcinoma of grade 1), the other subgroup to high grades (severe cervical dysplasia and carcinoma in situ, or papillary carcinoma of grade 3). Moderate dysplasia cases and grade 2 papillary carcinomas do not appear as separate intermediate categories but rather show values falling into the range of either the higher or lower grades. The margination values obtained from the cytological preparations corresponded well to those in the histological slides obtained from the resected specimens. In conclusion, nucleolar margination appears to be a feature which is easy to evaluate in a reproducible way and useful in cytological diagnosis.

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The number of nucleoli in benign and malignant thyroid lesions: a useful diagnostic sign in cytological preparations*

Cited by 10 publications

References 14 publications

RRS1 gene expression involved in the progression of papillary thyroid carcinoma

RRS1 gene expression involved in the progression of papillary thyroid carcinoma

Follicular Neoplasms of the Thyroid

Quantitative analysis of nucleolar margination in diagnostic cytopathology

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