The Number of Proliferating Cells in the Rostral Migratory Stream of Rat During the First Postnatal Month

Martončı́ková, Marcela; Račeková, Enikő; Orendáčová, J

doi:10.1007/s10571-006-9039-7

Cited by 19 publications

(24 citation statements)

References 20 publications

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“…Previously we have demonstrated that there are physiological changes in mitotic activity in the RMS during the first postnatal month. The lowest number of proliferating cells has been noted at day P7 [9]. In maternally deprived rats of the same age, the low density of dividing cells was even more striking (Figure 2).…”

Section: Cell Proliferationmentioning

confidence: 81%

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Early stress affects neurogenesis in the rat rostral migratory stream

et al. 2010

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Early stress affects neurogenesis in the rat rostral migratory stream Abstract: Stressful experience during the early postnatal period may influence processes associated with neurogenesis (i.e. proliferation, cell death, appearance of astrocytes or cell differentiation) in the neonatal rat rostral migratory stream (RMS). To induce stress, pups were subjected to maternal deprivation daily for three hours, starting from the first postnatal day till the seventh postnatal day. Immunohistochemical methods were used to visualize proliferating cells and astrocytes; dying cells and nitrergic cells were visualizedusinghistochemicalstaining.Quantitativeanalysisshowedthatmaternaldeprivationdecreasedthenumberofproliferating cells and significantly increased the number of dying cells in the RMS. Maternal deprivation did not influence the appearance of astrocytes in the RMS, but caused premature differentiation of nitrergic cells. In control rats, nitrergic cells can be observed in the RMS as early as the tenth postnatal day. In maternally deprived pups, these cells were detected as early as the seventh postnatalday. TheobservedearlierappearanceofnitrergiccellsintheRMSwasassociatedwithalteredproliferationandincreased cell dying and this observation supports the hypothesis that nitric oxide has an anti-proliferative role in the RMS. Our study demonstrates that maternal deprivation represents a stressful condition with a profound impact on early postnatal neurogenesis.

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Section: Cell Proliferationmentioning

confidence: 81%

“…The early postnatal RMS is U-shaped because the elbow between the vertical and horizontal arms is not as sharp compared to later postnatal stages. The typical "L"-shaped column, characteristic of the adult rat RMS, develops in two week-old rats [9].…”

Section: Introductionmentioning

confidence: 99%

Early stress affects neurogenesis in the rat rostral migratory stream

et al. 2010

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“…The proliferation activity of ependymal cells was assessed by the most commonly used markers of cell division: BrdU and Ki-67, respectively. BrdU immunohistochemistry has regularly been applied for estimating proliferating cells number in the main neurogenic region of the brain under physiological conditions [24] and in experimental interventions [25,26]. The more recently introduced marker, Ki-67 is also commonly used for detection of proliferation in the brain [27] and the spinal cord [16].…”

Section: Discussionmentioning

confidence: 99%

Regional differences of proliferation activity in the spinal cord ependyma of adult rats

et al. 2012

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Increased proliferation activity in the central canal ependyma of adult rodent spinal cord was described after injury and is thought to participate in recovery processes. Proliferation activity is scarce under physiological conditions, but still could be of importance, as in vitro studies showed that the spinal cord ependyma is an internal source of neural stem cells. Data from these studies indicate that there are regional differences in the distribution of proliferation activity along the rostro-caudal axis. We analyzed the proliferation activities in the ependyma within the entire extent of intact adult rat spinal cord. To identify proliferating cells we performed immunohistochemistry either for cell cycle S-phase marker BrdU or for the nuclear protein Ki-67. BrdU and Ki-67 positive cells were counted on sections selected from four spinal cord regions — cervical, thoracic, lumbar and sacral/coccygeal. Analysis showed that the number of BrdU positive cells within the ependyma was very low in all subdivisions of the spinal cord. Both BrdU and Ki-67 labeling revealed a significantly higher number of proliferating cells in the ependyma of sacrococcygeal part in comparison to all other spinal cord regions, suggesting that the caudal spinal cord might have potentially higher regeneration capacity compared to more rostral parts.

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“…This is consistent with findings of Uberti et al (2001), who detected a biphasic response to irradiation in dentate gyrus in mice, characterized by an early inhibition and delayed stimulation of cell proliferation. Later effects of ionizing radiation may involve the population of quiescent stem or precursor cells that appear to be responsible for repopulating of damaged SVZ Martončíková et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Under physiological conditions, this process is closely associated with simultaneous reduction in the number of stem or precursor cells via apoptosis (Brunjes & Armstrong 1996;Thomaidou et al 1997). The effects of ionizing radiation on the rat brain have been studied primarily in prenatal and neonatal animals (Shinohara et al 1997;Amano et al 2002) as well as in adults (Peissner et al 1999;Martončíková et al 2006). Acute low dose irradiation (0.5 Gy) has effect on various metabolic pathways that do not appear to be directly involved in cell death (Silasi et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Alterations in the rat forebrain apoptosis following exposure to ionizing radiation

et al. 2011

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Ionizing radiation commonly used in the radiotherapy of brain tumours can cause adverse side effects to surrounding normal brain tissue. The most significant response of adult brain to radiation damage is induction of apoptosis. The adult mammalian subventricular zone (SVZ) of the brain lateral ventricles (LV) and their subsequent lateral ventricular extension, the rostral migratory stream (RMS), is one of the few areas, which retains the ability to generate new neurons and glial cells throughout life. Taking into account the fact, that ionizing radiation is one of the strongest exogenous factors affecting cell proliferation, the aim of the present study was to investigate the occurrence of radiation-induced apoptosis in this neurogenic region. Adult male Wistar rats were investigated 1, 5 or 10 days after single whole-body gamma irradiation with the dose of 3 Gy. Apoptotic cell death was determined by in situ labelling of DNA nick ends (TUNEL) and fluorescence microscopy evaluation of TUNEL-positive cells. Considerable increase of apoptotic TUNEL-positive cells was observed 24 hrs after irradiation in caudal parts of RMS; i.e. in the vertical arm and elbow of RMS. Initial increase was followed by strong reduction of apoptosis in the RMS and by secondary over-accumulation of apoptotic cells in the animals that survived ten days after exposure. Results showed, that the proliferating population of cells, arisen in SVZ are highly sensitive to radiation-induced apoptosis. This observation should have implications for clinical radiotherapy to avoid complications in therapeutic brain irradiation.

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The Number of Proliferating Cells in the Rostral Migratory Stream of Rat During the First Postnatal Month

Cited by 19 publications

References 20 publications

Early stress affects neurogenesis in the rat rostral migratory stream

Early stress affects neurogenesis in the rat rostral migratory stream

Regional differences of proliferation activity in the spinal cord ependyma of adult rats

Alterations in the rat forebrain apoptosis following exposure to ionizing radiation

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