The Nutritional Significance of Lipid Rafts

Yaqoob, Parveen

doi:10.1146/annurev-nutr-080508-141205

Cited by 95 publications

(69 citation statements)

References 176 publications

(181 reference statements)

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“…[23,24] Furthermore, incorporation of ω-3 PUFAs into cell phospholipid membranes changes the fluidity, structure and function of lipid rafts, resulting in altered downstream signaling by cell surface receptors, such as epidermal growth factor receptor. [25,26] Moreover, ω-3 PUFAs are highly peroxidizable and increase levels of intracellular reactive oxygen species (ROS), a byproduct of cell growth. Although moderately increased levels of ROS damage DNA and promote mutagenesis in cells, recent evidence indicates that high ROS levels exert an oxidative stress that can restrain tumor progression and metastasis by causing cell senescence or death.…”

Section: Discussionmentioning

confidence: 99%

658 Marine Omega-3 Polyunsaturated Fatty Acid Intake and Survival After Colorectal Cancer Diagnosis

Song¹,

Zhang²,

Meyerhardt³

et al. 2016

Gastroenterology

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Objective-Experimental evidence supports an antineoplastic activity of marine omega-3 polyunsaturated fatty acids (ω-3 PUFAs; including eicosapentaenoic acid, docosahexaenoic acid, and docosapentaenoic acid). However, the influence of ω-3 PUFAs on colorectal cancer (CRC) survival is unknown. AUTHOR CONTRIBUTIONSDrs Song and Chan have full access to all of the data in the study, and take responsibility for the integrity of the data and the accuracy of the data analysis. HHS Public AccessAuthor manuscript Gut. Author manuscript; available in PMC 2017 October 01. Author Manuscript Author ManuscriptAuthor Manuscript Author ManuscriptDesign-Within the Nurses' Health Study and Health Professionals Follow-up Study, we prospectively studied CRC-specific and overall mortality in a cohort of 1,659 CRC patients according to intake of marine ω-3 PUFAs and its change after diagnosis.Results-Higher intake of marine ω-3 PUFAs after CRC diagnosis was associated with lower risk of CRC-specific mortality (P for trend=0.03). Compared with patients who consumed less than 0.10 g/day of marine ω-3 PUFAs, those consuming at least 0.30 g/day had an adjusted hazard ratio for CRC-specific mortality of 0.59 (95% confidence interval [CI], 0.35 to 1.01). Patients who increased their marine ω-3 PUFA intake by at least 0.15 g/day after diagnosis had a hazard ratio of 0.30 (95% CI, 0.14 to 0.64, P for trend<0.001) for CRC deaths, compared with those who did not change or changed their intake by less than 0.02 g/day. No association was found between postdiagnostic marine ω-3 PUFA intake and all-cause mortality (P for trend=0.47).Conclusion-High marine ω-3 PUFA intake after CRC diagnosis is associated with lower risk of CRC-specific mortality. Increasing consumption of marine ω-3 PUFAs after diagnosis may confer additional benefits to patients with CRC.

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Section: Discussionmentioning

confidence: 99%

658 Marine Omega-3 Polyunsaturated Fatty Acid Intake and Survival After Colorectal Cancer Diagnosis

Song¹,

Zhang²,

Meyerhardt³

et al. 2016

Gastroenterology

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“…Plasma membrane microdomains are likely to be of diverse nature, exhibiting altering substable L o states (9,(47)(48)(49)(50)(51)(52). Therefore, the DRM/DSF protein abundance ratios can better reflect global changes in protein-sterol interactions and account for total protein abundance changes compared with studying abundance changes in DRM only.…”

Section: Discussionmentioning

confidence: 99%

Unraveling Sterol-dependent Membrane Phenotypes by Analysis of Protein Abundance-ratio Distributions in Different Membrane Fractions Under Biochemical and Endogenous Sterol Depletion

Zauber

Szymanski

Schulze

2013

Molecular & Cellular Proteomics

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During the last decade, research on plasma membrane focused increasingly on the analysis of so-called microdomains. It has been shown that function of many membrane-associated proteins involved in signaling and transport depends on their conditional segregation within sterol-enriched membrane domains. High throughput proteomic analysis of sterol-protein interactions are often based on analyzing detergent resistant membrane fraction enriched in sterols and associated proteins, which also contain proteins from these microdomain structures. Most studies so far focused exclusively on the characterization of detergent resistant membrane protein composition and abundances. This approach has received some criticism because of its unspecificity and many co-purifying proteins. In this study, by a label-free quantitation approach, we extended the characterization of membrane microdomains by particularly studying distributions of each protein between detergent resistant membrane and detergent-soluble fractions (DSF). This approach allows a more stringent definition of dynamic processes between different membrane phases and provides a means of identification of co-purifying proteins. We developed a random sampling algorithm, called Unicorn, allowing for robust statistical testing of alterations in the protein distribution ratios of the two different fractions. Unicorn was validated on proteomic data from methyl-␤-cyclodextrin treated plasma membranes and the sterol biosynthesis mutant smt1. The plasma membrane incorporates a broad spectrum of proteins covering mainly different structural, signaling or transport functionalities. Being the first semipermeable cell barrier to its surrounding environment the plasma membrane is important for metabolite transport as well as initiation point of several signaling processes (1-4). To maintain cell homeostasis, protein activity as well as complex formation through protein protein interactions (PPI) need to be tightly regulated. The major regulating mechanisms are postranslational modification of proteins and modulated abundances of proteins present in the plasma membrane. Another potential regulating mechanism became apparent with the discovery of sterol and sphingolipid enriched domains (microdomains) in the plasma membrane (5-8, 3). Microdomain like structures have been shown to form spontaneously in artificial plasma membranes (9). After a decade of research on these structures, microdomains turned out to be particularly involved in signaling and transport processes incorporating a specific set of proteins. Microdomains provide subcompartments in the plasma membrane with specific physicochemical properties that on specific sterol protein interactions might alter protein activity or PPIs. With the discovery of microdomains the fluid lipid mosaic model was extended by distinguishing two plasma membrane phases, an ordered phase of lower density (L o phase) enriched in sterols, sphingolipids and long chain fatty acids and a disordered phase of higher density (L d phase). From isolated...

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“…However, it is not defined yet if targeting lipid rafts in vivo might impair physiological functions. For instance, it is not clear whether dietary LC-PUFA are incorporated into raft lipids or whether their low affinity to cholesterol disallows this and causes phase separation from rafts and displacement of raft proteins [128]. Moreover, it was shown that depletion of cholesterol leads to instability of surface AMPA-glutamate in lipid rafts, which was accompanied by gradual loss of synapses and dendritic spines [129].…”

Section: Discussionmentioning

confidence: 99%

Manipulation of Lipid Rafts in Neuronal Cells~!2009-09-18~!2009-12-16~!2010-03-19~!

Eckert¹

2010

TOBIOJ

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Lipid rafts are specialized plasma membrane micro-domains highly enriched in cholesterol, sphingolipids and glycosylphosphatidylinositol (GPI) anchored proteins. Lipid rafts are thought to be located in the exofacial leaflet of plasma membranes. Functionally, lipid rafts are involved in intracellular trafficking of proteins and lipids, secretory and endocytotic pathways, signal transduction, inflammation and in cell-surface proteolysis. There has been substantial interest in lipid rafts in brain, both with respect to normal functioning and with certain neurodegenerative diseases. Based on the impact of lipid rafts on multitude biochemical pathways, modulation of lipid rafts is used to study related disease pathways and probably offers a target for pharmacological intervention. Lipid rafts can be targeted by modulation of its main components, namely cholesterol and sphingolipids. Other approaches include the modulation of membrane dynamics and it has been reported that protein-lipid interactions can vary the occurrence and composition of these membrane micro-domains. The present review summarizes the possibilities to modulate lipid rafts with focus on neuronal cells.

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The Nutritional Significance of Lipid Rafts

Cited by 95 publications

References 176 publications

658 Marine Omega-3 Polyunsaturated Fatty Acid Intake and Survival After Colorectal Cancer Diagnosis

658 Marine Omega-3 Polyunsaturated Fatty Acid Intake and Survival After Colorectal Cancer Diagnosis

Unraveling Sterol-dependent Membrane Phenotypes by Analysis of Protein Abundance-ratio Distributions in Different Membrane Fractions Under Biochemical and Endogenous Sterol Depletion

Manipulation of Lipid Rafts in Neuronal Cells~!2009-09-18~!2009-12-16~!2010-03-19~!

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