The nutritional supplement FFD-002 attenuates streptozotocin-induced diabetes and decelerates diabetes-related tissue injury

Abstract

A nutritional supplement FFD-002 composed of micronutrients, macronutrients, and herbal actives was formulated with ingredients known for therapeutic effect in diabetes management. The study evaluated the effect of supplementing FFD-002 on diabetes and related complications. Diabetes was induced in Wistar rats by administering a single dose of streptozotocin (STZ, 45 mg/Kg bodyweight, intraperitoneal) and rats were supplemented with either 2.5 g/kg or 5 g/kg of FFD-002 (p. o [per oral] once daily) for 90 days. Supplementation of FFD-002 to diabetic rats resulted in improved fasting glucose levels and marked decrease in diabetes-induced nephropathy and retinopathy. Cell-based experimental results indicated that FFD-002 treatment increased fat oxidation in muscle cells and stimulated the secretion of GLP-1 (Glucagon-Like Peptide-1) in intestinal cells.These studies showed that FFD-002, formulated by combining modern nutritional concepts with phytomedicine, could be beneficial in the management of diabetes.

Treatment with a polyherbal extract improves fat metabolism, attenuates hepatic stellate cell activation and fibrogenesis

“…After 24 h incubation, supernatants were discarded and cells were lysed with lysis buffer containing protease inhibitor cocktail. Cell lysates were centrifuged at 1000xg for 10 min; the clear supernatant was used for estimating the enzyme activity [10]. The enzyme activity was normalized to protein levels.…”