The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the dog

Cook, James L.; Kuroki, Keiichi; Visco, Denise M.; Pelletier, J.-P.; Schulz, Laura C.; Lafeber, Floris P. J. G.

doi:10.1016/j.joca.2010.04.017

Cited by 182 publications

(256 citation statements)

References 68 publications

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“…Prior to AC collection, the articular surface of each femoral condyle was visually inspected to ensure the absence of OA. 25 Four cylindrical AC plugs attached with subchondral bones (A3 × 3 mm) were punched from the load bearing region of each femoral condyle. Despite only six joints being used, a total of 24 AC plugs were collected for the experiment.…”

Section: Specimen Preparationmentioning

confidence: 99%

“…25,26,46 Since the coefficient of RI fluctuation demonstrated the capability to detect the PG loss of AC, it could be potentially incorporated into preclinical applications. In particular, the histological assessment shows that the AC samples are either healthy or merely degenerated in early stage, which suggests the potential of RI fluctuation for the diagnose of early OA.…”

Section: Ri Fluctuation and Pathological Progressmentioning

confidence: 99%

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Depth-dependent refractive index of normal and early degenerated articular cartilage

Wang¹,

Wu²,

Kirk³

2013

J. Biomed. Opt

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Abstract. The depth distributions of refractive index (RI) in normal and early degenerated articular cartilage (AC) were measured and correlated to the pathological statuses. Utilizing a confocal microscope, the depth distribution of RI was determined with an interval of 50 μm from the articular surface and approximated as a smooth curve. The fluctuation of RI was quantified with a specially defined coefficient and the proteoglycan (PG) loss was quantified with histological images. The overall RI of the AC collected from the femoral condyles of mature sheep was 1.4444. Significant differences existed in the refractive indices of different cartilage samples or even in the different depths of the same sample. The RI of normal cartilage distributed as a regular ramp or arch curve with depth and peaked in the middle-deep zone. The cartilage samples detected with high PG losses were coupled with severe fluctuations of RI. The fluctuating level was increased with pathological progress. The coefficient of RI fluctuation can distinguish the normal cartilage from the initially degenerated one, which potentially provides an indicator to detect the pathological development of AC. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Specimen Preparationmentioning

confidence: 99%

Section: Ri Fluctuation and Pathological Progressmentioning

confidence: 99%

Depth-dependent refractive index of normal and early degenerated articular cartilage

Wang¹,

Wu²,

Kirk³

2013

J. Biomed. Opt

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“…The gradation of the samples was performed in the same way as in a previously published article [19] The synovial membrane and the decalcified bone and cartilage core biopsies were evaluated by two independent observers according to the already published guidelines [22,23]. The gradation of damage to the structures was as follows: in cartilage, chondrocyte and proteoglycan pathology from 0 (normal) to 4 (completely affected) and in tidemark and synovial membrane from 0 (normal) to 2 (osteoarthritic).…”

Section: Microscopic Evaluationmentioning

confidence: 99%

Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model

Permuy

López

Miño

et al. 2012

Bone

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Background: Osteoarthritis is thought to be the most prevalent chronic and disabling joint disease in animals and humans. At present, there is no ideal treatment option. The aim of this study was to assess the effects of the treatment with oral diacerein on articular cartilage, synovial membrane and subchondral bone in an experimental rabbit model of osteoarthritis by micro-CT evaluation and histological analysis. To this purpose, osteoarthritis was surgically induced on one knee of 16 rabbits using the contralateral knee as healthy controls. Treatment was started three weeks later and lasted eight weeks. Animals were divided into two groups for treatment: Placebo (treated daily with oral saline) and diacerein (treated orally with 1.5 mg/kg/day of diacerein).

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“…Recently, a blind study of the gross anatomic disease progression in these canine joints has been completed (26). Using the OARSI scores of 0-4 that graded different parts of a joint (27), the 12-week contralateral tibias had a score of 1, while the 12-week transected tibias (OA) had a score of 2; the difference was statistical significant. After excess tissues (muscles and skins) were trimmed, all unopened knee capsules were placed in a sample holder and imaged (within 24 hours of sacrifice) in a Varian MRI system with a 7T/20 cm horizontal magnet (Santa Clara, CA, USA)-the system is termed as macro-MRI in this report.…”

Section: Sample and Proceduresmentioning

confidence: 99%

Image interpolation improves the zonal analysis of cartilage T2 relaxation in MRI

Badar

Xia

2017

Quant. Imaging Med. Surg.

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Background: This project aimed to investigate the improvement in the detection of osteoarthritis (OA) in cartilage by the interpolation of T2 images, in the situation when the native MRI resolution is insufficient to resolve the depth-dependent T2 characteristics in articular cartilage (AC).Methods: Eighteen intact canine knee joints that were healthy or had mild (contralateral) or severe OA were T2-imaged in a 7T/20 cm MRI system at 200 µm/pixel resolution (macro-MRI). Two image analysis methods were used to interpolate the images to 100 µm/pixel, i.e., by Fourier-transforming the time-domain FID (Free Induction Decay) signal using the Varian NMR software and by interpolating the 2D T2 image using the ImageJ software. and the interpolated 100 µm resolutions were subdivided into two equal-thickness regions and three-equal thickness regions based on clinical MRI protocols. A new method divided the T2 profiles into three-unequal thickness zones according to the T2 profiles at 17.6 µm/pixel from the same cartilage imaged in a 7 Tesla/9 cm µMRI system. Both interpolation methods improved the depth-dependent T2 images/profiles in macro-MRI. The unequal zone division in T2 had better OA sensitivity than the equal zone division. The threeequal zone division of T2 profiles had better OA sensitivity than the two-equal zone division. The statistical significant difference between the healthy and mild OA cartilage is detected (P=0.0018) only by the unequal zone division method at 100 µm resolution.Conclusions: Data interpolation improves the T2 sensitivity in MRI of cartilage OA. Unequal division of tissue thickness enables better early stage of OA detection than the equal division.

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The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the dog

Cited by 182 publications

References 68 publications

Depth-dependent refractive index of normal and early degenerated articular cartilage

Depth-dependent refractive index of normal and early degenerated articular cartilage

Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model

Image interpolation improves the zonal analysis of cartilage T2 relaxation in MRI

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