The occurrence and distribution of two phosphofructokinase proteins in the facultative anaerobic family of enterobacteriaceae

The Pasteur Effect in Escherichia Coli K-12 Mutants Lacking the Allosteric Form of Phosphofructokinase

“…It constitutes less than 10% of the total phosphofructokinase synthesis in E. coli [7,8], is not subject to synthetic suppression but can exist in either of two interconvertible forms, depending upon the presence or absence of ADP, fructose 6-phosphate and ATP [9J. These results seriously questioned earlier assumptions [ 10-1 21 that the allostery of phosphofructokinase plays a vital role, as a regulatory enzyme, in determining whether fermentation or respiration takes place [3,4,13,14]. Robinson and Fraenkel [14] indicated that the presence of the allosteric type of phosphofructokinase does not make much difference to growth rate or to the yield of this organism when Gowing on glucose (Yp).…”

“…The existence of two forms of phosphofructokinase in Escherichiacoli [ 1,2] and a number of other genera of the facultative anaerobic family of Enterobacteriaceae [3] has been well established. Whereas the allosteric form responds to changes in dissolved oxygen tension [4-61, the non-allosteric form varies little with culture condition [S].…”