The occurrence and diversity of antibiotic resistance and virulence factor genes in wastewater from four North American treatment plants

Le, Loan T.; Huang, Zhuojin; Whiteson, Katrine; Jiang, Sunny C.

doi:10.1039/d1ew00820j

Cited by 3 publications

(2 citation statements)

References 59 publications

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“…Wastewater treatment plants have been identified as a hotspot for enriching AMR and spreading ARB and antibiotic-resistant genes (ARG) into the environment due to the inputs of pharmaceuticals and antibiotics from municipal and industrial sources, and the high density of bacteria in biological treatment to facilitate horizontal gene transfer of ARG. − Due to such conditions, the role of wastewater treatment in the transport and fate of AMR is now a research focus area, − but the risk of wastewater reuse in spreading AMR infections is less understood. The nonpotable reuse or recycling of treated wastewater, referred to as reclaimed wastewater, is applied to flush toilets, industrial processes for cooling, used to irrigate parks, golf courses, or agriculture, or utilized for fountains or ornamental water features.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Microbial Risk Assessment of Antibiotic-Resistant E. coli, Legionella pneumophila, and Mycobacteria in Nonpotable Wastewater Reuse Applications

Quon,

Jiang

2024

Environ. Sci. Technol.

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Antibiotic-resistant bacteria (ARB) have become a major threat to public health and modern medicine. A simple death kinetics-based dose−response model (SD-DRM) was incorporated into a quantitative microbial risk assessment (QMRA) to assess the risks of exposure to reclaimed wastewater harboring antibiotic-resistant E. coli, Legionella pneumophila, and Mycobacterium avium for multiple exposure scenarios. The fractions of ARB and trace antibiotics present in the body were incorporated to demonstrate their impact on infection risks. Both ARB and antibiotic susceptible bacteria, ASB, are assumed to have the same dose−response in the absence of antibiotics but behave differently in the presence of residual antibiotics in the body. Annual risk of L. pneumophila infection exceeded the EPA 10 −4 pppy (per person per year) benchmark at concentrations in reclaimed water greater than 10 3 −10 4 CFU/L, depending on parameter variation. Enteropathogenic E. coli infection risks meet the EPA annual benchmark at concentrations around 10 5 −10 6 total E. coli. The results illustrated that an increase in residual antibiotics from 0 to 40% of the minimum inhibitory concentration (MIC) reduced the risk by about 1 order of magnitude for E. coli but was more likely to result in an untreatable infection.

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Section: Introductionmentioning

confidence: 99%

Quantitative Microbial Risk Assessment of Antibiotic-Resistant E. coli, Legionella pneumophila, and Mycobacteria in Nonpotable Wastewater Reuse Applications

Quon,

Jiang

2024

Environ. Sci. Technol.

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“…Wastewater treatment plants (WWTPs) serve not only as collection points for antibiotics from sewer networks but also play an important role in degrading and removing them before the water is discharged into the environment or is supplied for various reuse applications. However, several studies have indicated that removal efficiencies of antibiotics in WWTPs are usually low. − The wastewater antibiotics may also amplify the antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARG) during biological wastewater treatment such that treated wastewater becoming an important source of antibiotics, ARB, and ARG entering the environment and reused water. Antibiotics in an aquatic environment, even at low concentrations, can promote the breeding of ARB through mutation or horizontal gene transfer, allowing microorganisms to survive in the presence of antibiotics .…”

Section: Introductionmentioning

confidence: 99%

Sensing Antibiotics in Wastewater Using Surface-Enhanced Raman Scattering

Huang

Hong

Santiago

et al. 2023

Environ. Sci. Technol.

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Rapid and cost-effective detection of antibiotics in wastewater and through wastewater treatment processes is an important first step in developing effective strategies for their removal. Surface-enhanced Raman scattering (SERS) has the potential for label-free, real-time sensing of antibiotic contamination in the environment. This study reports the testing of two gold nanostructures as SERS substrates for the label-free detection of quinoline, a small-molecular-weight antibiotic that is commonly found in wastewater. The results showed that the self-assembled SERS substrate was able to quantify quinoline spiked in wastewater with a lower limit of detection (LoD) of 5.01 ppb. The SERStrate (commercially available SERS substrate with gold nanopillars) had a similar sensitivity for quinoline quantification in pure water (LoD of 1.15 ppb) but did not perform well for quinoline quantification in wastewater (LoD of 97.5 ppm) due to interferences from non-target molecules in the wastewater. Models constructed based on machine learning algorithms could improve the separation and identification of quinoline Raman spectra from those of interference molecules to some degree, but the selectivity of SERS intensification was more critical to achieve the identification and quantification of the target analyte. The results of this study are a proof-of-concept for SERS applications in label-free sensing of environmental contaminants. Further research is warranted to transform the concept into a practical technology for environmental monitoring.

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Increasing Prevalence of Antibiotic-Resistant Genes in Wastewater: Impact on Public Health

Kour,

Singh,

Khera

2023

Genomics of Antibiotic Resistant Bacteria in Industrial Waste Water Treatment

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The occurrence and diversity of antibiotic resistance and virulence factor genes in wastewater from four North American treatment plants

Abstract: The spread of antibiotic resistance in aquatic environments is an emerging environmental issue due to potential threats to public health. Wastewater treatment plants (WWTPs) could serve as a sink for...

Cited by 3 publications

References 59 publications

Quantitative Microbial Risk Assessment of Antibiotic-Resistant E. coli, Legionella pneumophila, and Mycobacteria in Nonpotable Wastewater Reuse Applications

Quantitative Microbial Risk Assessment of Antibiotic-Resistant E. coli, Legionella pneumophila, and Mycobacteria in Nonpotable Wastewater Reuse Applications

Sensing Antibiotics in Wastewater Using Surface-Enhanced Raman Scattering

Increasing Prevalence of Antibiotic-Resistant Genes in Wastewater: Impact on Public Health

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