The occurrence of a cyanocobalamin-binding protein in milk and the isolation of a cyanocobalamin-protein complex from sow's milk

Gregory, Margaret E.; Holdsworth, E. S.

doi:10.1042/bj0590329

Cited by 42 publications

(22 citation statements)

References 6 publications

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“…Thus, it was shown that as compared with British or American mothers, the vitamin A, thiamine, riboflavine, and vitamin C content of milk from poor Indian mothers was low (Macy, 1949;Macy, Kelly, and Sloan, 1950;Kon and Mawson, 1950;Gunther and Stanier, 1951). Our studies indicate that the vitamin B12 content of milk of Indian mothers is also very low as compared to values reported by Westem workers (Collins et al, 1951;Gregory and Holdsworth, 1955;Karlin, 1956;Davidov and Kruglova, 1960) (Table IV). Even though vegetarian subjects had low serum vitamin B12 levels, their milk vitamin B12 values were not significantly low as compared to those of non-vegetarian mothers.…”

Section: Discussioncontrasting

confidence: 44%

Maternal Milk and Serum Vitamin B12, Folic Acid, and Protein Levels in Indian Subjects

Jathar¹,

Kamath²,

Parikh³

et al. 1970

Archives of Disease in Childhood

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Section: Discussioncontrasting

confidence: 44%

Maternal Milk and Serum Vitamin B12, Folic Acid, and Protein Levels in Indian Subjects

Jathar¹,

Kamath²,

Parikh³

et al. 1970

Archives of Disease in Childhood

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“…General The folate binding in milk exemplifies a type of passive trapping process that is already familiar from thc literature on the protein binding of vitamin B,, in milk (Gregory, 1954;Gregory & Holdsworth, 1955) and of steroid hormones in blood (cf. Jensen &Jacobson, 1962; Briggs & Brotherton, 1970).…”

Section: Whole Body Folate In the Kidmentioning

confidence: 99%

Folate nutrition in the kid

Ford¹,

Knaggs²,

Salter³

et al. 1972

Br J Nutr

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I. A study was made on the folate content of goat's milk in relation to stage of lactation, and on the interrelationship between blood and milk folate concentrations in the dam and in the kid.2. In seven goats the folate concentration in the colostrum at parturition ranged from 136 to 300 ng/ml, and averaged 205 ng/ml. The concentration fell sharply during the early days of lactation and by day 14 it averaged only 9-5 nglml. This pattern of rapid decline in milk folate concentration with advancing lactation was little affected by daily provision of folic acid parenterally in relatively large amounts.3. The colostrum and milk contained a minor whey protein that combined strongly with folate, and presumably acts as a trapping mechanism to accumulate the vitamin from the plasma into the milk. The milk folate concentration is determined by the rate of milk secretion in relation to the availability of free folate in the blood plasma. 4.At parturition the plasma folate concentration in the kids was very low -about I nglmlbut by day 2 it had increased to about 28 ng/ml. This folate was protein-bound and accompanied by an excess of free binder protein. It appeared that the folate-protein complex from the colostrum was transmitted intact into the kids' blood circulation.5. The possible importance of the folate-binding protein in the regulation of folate metabolism is discussed.Goat's milk is a comparatively poor source of folate. It contains about 6 n g / d as against 50 ng/ml in human milk and cow's milk, and its prolonged consumption as the sole diet by suckling infants may cause 'goat's milk anaemia', which is now recognized as being a megaloblastic anaemia symptomatic of folate dcficiency (Becroft & Holland, 1966). In man the metabolic turnover of folate is rapid and any severe restriction of dietary intake is quickly followed by a fall in serum folate and a slower progressive fall in red cell folate (Herbert, 1962). Thcse observations prompted speculation on the folate nutrition of the newborn kid, and the present papcr reports on the folate content of goat's milk in relation to stage of lactation, and on some interrelationships between blood and milk folate concentrations in the dam and in the kid. E X P E R I M E N T A LPedigree British Saanen goats of the Institute herd were used. They were maintained on clover hay and concentrates and given daily access to pasture. They were brought indoors each evening and housed on concrete in individual pens. On the expected day of parturition and for about a week thereafter the goats were kept indoors. The kids remained in the pens for 3 weeks, during which time they were suckled by their dams and also had access to hay and concentrates.Some of the goats were milked pre-partum, as deemed necessary by the goatherd, https://www.cambridge.org/core/terms. https://doi

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“…fluids such as milk (Gregory & Holdsworth, 1955), tears, saliva, urine, amniotic fluid and bile (Glass, 1974;Grasbeck, Visuri & Stenman, 1972;Kumar, Rathi & Meyer, 1976), and tissues such as pyloric mucosa, salivary glands (Grasbeck & Visuri, 1968;Hurliman & Zuber, 19691, erythrocytes and granulocytes (Grasbeck, 1969). On Sephadex G-200, transcobalamin I elutes with apparent molecular weight 120000 (Hom, Olesen & Lous, 1966;Stenman, Simons & Grasbeck, 1968; because the branched side chains retard elution, the true molecular weight being 56000 by sedimentation equilibration ultracentrifugation and 58 200 by amino acid and carbohydrate analysis (Allen & Majerus, 1972a).…”

Section: Introductionmentioning

confidence: 99%

Distribution of Endogenous Cobalamin between the Transcobalamins in Various Mammals

et al. 1979

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1. Plasma samples from ten mammals were chromatographed on Sephadex G-200 columns and the total cobalamin content of each fraction was determined. 2. Unlike the situation in man, the bulk of the endogenous plasma cobalamin was found attached to a transcobalamin II-like protein in all ten animals. Transcobalamin 0 carried between 3 and 20% and this proportion appeared to be inversely related to the plasma total cobalamin. No endogenous cobalamin peak corresponding to transcobalamin I was detected in any species, though in the rabbit 5.3% of the plasma total cobalamin was attached to a protein of apparent molecular weight 176 000.

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The occurrence of a cyanocobalamin-binding protein in milk and the isolation of a cyanocobalamin-protein complex from sow's milk

Cited by 42 publications

References 6 publications

Maternal Milk and Serum Vitamin B12, Folic Acid, and Protein Levels in Indian Subjects

Maternal Milk and Serum Vitamin B12, Folic Acid, and Protein Levels in Indian Subjects

Folate nutrition in the kid

Distribution of Endogenous Cobalamin between the Transcobalamins in Various Mammals

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