The Occurrence of Bioactive Micromonosporae in Aquatic Habitats of the Sunshine Coast in Australia

Eccleston, Glen P.; Brooks, Peter; Kurtböke, D. İpek

doi:10.3390/md20080012

Cited by 29 publications

(19 citation statements)

References 50 publications

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“…Hong et al (2009) noted that these results were not only interesting but also would guide future bioprospecting strategies. Consistent with the observations of the investigators (Eccleston et al 2008;Hong et al 2009), our study revealed that the microbial ecology of the sampling sites of the Sundarbans was influenced by the chemical nature of the soil. However, positive significant correlation between AP and total soil nitrogen content and not organic carbon was not been reported earlier.…”

Section: Discussionsupporting

confidence: 91%

“…The investigation by Eccleston et al (2008) provided interesting ecological insights. Mangrove mud sediments yielded relatively high populations of gentamicin-resistant actinomycetes compared to benthic communities associated with littoral sand sediments, freshwater creek and lake habitats.…”

Section: Discussionmentioning

confidence: 99%

“…Estuarine mangroves were not systematically investigated for bioactive actinomycetes following the ecological principles until recently. Eccleston et al (2008) studied the ecological distribution and frequency of bioactive Micromonosporae with particular emphasis on gentamycin production in several aquatic habitats of the Sunshine Coast region of Australia. Hong et al (2009) isolated, characterized and screened actinomycetes from soil and plant material collected from eight mangrove sites of China.…”

Section: Introductionmentioning

confidence: 99%

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Phylogeny, phenotypic and nutritional characteristics of estuarine soil actinomycetes having broad-spectrum antimicrobial activity derived from an ecologically guided bioprospecting programme

Mitra

Pramanik

Santra

et al. 2010

World J Microbiol Biotechnol

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No investigation has been done to identify ecological factors selecting for broad-spectrum antimicrobial activity of actinomycetes in the estuaries. Previously, we established that, in the Sundarbans (the world's largest tidal mangrove forest), high antagonistic potential (AP) sampling sites were influenced by tides, while the low AP sites were not. We now report molecular phylogenetic analysis, morphological, physiological and biochemical characteristics of actinomycetes of high AP sites. The effects of soil organic carbon, nitrogen and ionic content (which strongly influenced AP) on the strength and spectrum of activity of the isolates were also studied in shake flasks. Molecular phylogenetic analysis showed sequences of our strains to be 96-99% similar to the 16S rDNA sequences of Streptomyces. Results showed variation among sporophore sizes, ornamentation and number of spores. Jaccard's similarity coefficients of the isolates varied from 0.512 to 0.884 indicating disparity in the biochemical and physiological characteristics, possibly due to spatial separation of the sampling sites. Top soil in the intertidal zone of estuaries is generated from settling and consolidation of fluid mud and Streptomyces would be expected to produce broad-spectrum antimicrobials in such virgin soil. Isolates should be collected from the narrow band between the mean high and low tide marks to maximize chances of finding broad-spectrum activity. Considering the study on nutritional requirements vis-à-vis the field studies, it was concluded that results of this investigation corroborated the field observations where soil nitrogen, rather than organic carbon or ionic concentration, played a major role in determining the antimicrobial spectrum.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogeny, phenotypic and nutritional characteristics of estuarine soil actinomycetes having broad-spectrum antimicrobial activity derived from an ecologically guided bioprospecting programme

Mitra

Pramanik

Santra

et al. 2010

World J Microbiol Biotechnol

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“…Several reports from different geographical locations around the world have described the occurrences of actinomycetes in different mangrove habitats. Eccleston et al (2008) reported the occurrence of actinomycetes belongs to genus Micromonospora from the Sunshine Coast in Australia. A rifamycin-producing Micromonospora from mangroves of the South China Sea was reported by Huang et al (2008) and Xie et al (2006).…”

Section: Actinomycetesmentioning

confidence: 99%

Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: a review

Thatoi

Behera

Mishra³

et al. 2012

Ann Microbiol

265

156

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Mangrove forests occurring at the interface of terrestrial and marine ecosystems represent a rich biological diversity of plants, animals and microorganisms. Microbes, being an important component of the mangrove environment, not only play a very critical role in creating and maintaining this biosphere but also serve as a source of biotechnologically valuable and important products. By participating in various steps of decomposition and mineralization of leaf litter, microbes make an essential contribution to the productivity of the mangrove ecosystem. They able to recycle nutrients, produce and consume gases that affect global climate, destroy pollutants, treat anthropogenic wastes and can also be used for biological control of plant and animal pests. Microorganisms from mangrove environments are a major source of antimicrobial agents and also produce a wide range of important medicinal compounds, including enzymes, antitumor agents, insecticides, vitamins, immunosuppressants, and immune modulators. However, the phylogenetic and functional description of microbial diversity in mangrove ecosystems has not been addressed to the same extent as for other environments. Even though the mangrove ecosystem is very rich in microbial diversity, less than 5% of species have been described; in many cases neither their ecological role nor their application potential is known. Recently developed technologies in molecular biology and genetics offer great promise to explore the potential of microbial diversity. Hence, the present paper makes an attempt to review the microbial diversity in mangrove ecosystems and explore their potential applications in various fields such as agriculture, pharmaceutical, industrial, environmental and medical sciences.

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“…such as glycerol asparagine agar, actinomycetes isolation agar (Nanjwade et al, 2010), tryptone yeast extract medium (ISP1), yeast extract malt extract agar (ISP2) (Busarakam et al, 2014) and Czapek dox agar (Mangamuri et al, 2016). The media were supplemented with gentamicin (1 μg/ml) and fluconazole (50 μg/ml) after sterilization to inhibit the growth of bacteria and fungi, respectively (Eccleston et al, 2008). The plates were then incubated for 21 days at 30°C.…”

Section: Methodsmentioning

confidence: 99%

Bioactive Molecule from Streptomyces sp. Mitigates MDR Klebsiella pneumoniae in Zebrafish Infection Model

et al. 2017

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The emergence and spread of multi-drug resistant (MDR) especially carbapenem-resistant Klebsiella pneumoniae is a major emerging threat to public health, leading to excess in mortality rate as high as 50–86%. MDR K. pneumoniae manifests all broad mechanisms of drug resistance, hence development of new drugs to treat MDR K. pneumoniae infection has become a more relevant question in the scientific community. In the present study a potential Streptomyces sp. ASK2 was isolated from rhizosphere soil of medicinal plant. The multistep HPLC purification identified the active principle exhibiting antagonistic activity against MDR K. pneumoniae. The purified compound was found to be an aromatic compound with aliphatic side chain molecule having a molecular weight of 444.43 Da. FT-IR showed the presence of OH and C=O as functional groups. The bioactive compound was further evaluated for drug induced toxicity and efficacy in adult zebrafish infection model. As this is the first study on K. pneumoniae – zebrafish model, the infectious doses to manifest sub-clinical and clinical infection were optimized. Furthermore, the virulence of K. pneumoniae in planktonic and biofilm state was studied in zebrafish. The MTT assay of ex vivo culture of zebrafish liver reveals non-toxic nature of the proposed ASK2 compound at an effective dose. Moreover, significant increase in survival rate of infected zebrafish suggests that ASK2 compound from a new strain of Streptomyces sp. was potent in mitigating MDR K. pneumoniae infection.

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The Occurrence of Bioactive Micromonosporae in Aquatic Habitats of the Sunshine Coast in Australia

Cited by 29 publications

References 50 publications

Phylogeny, phenotypic and nutritional characteristics of estuarine soil actinomycetes having broad-spectrum antimicrobial activity derived from an ecologically guided bioprospecting programme

Phylogeny, phenotypic and nutritional characteristics of estuarine soil actinomycetes having broad-spectrum antimicrobial activity derived from an ecologically guided bioprospecting programme

Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: a review

Bioactive Molecule from Streptomyces sp. Mitigates MDR Klebsiella pneumoniae in Zebrafish Infection Model

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