The occurrence of blaTEM, blaSHV and blaOXA genotypes in Extended-Spectrum β-Lactamase (ESBL)-producing Pseudomonas aeruginosa strains in Southwest of Iran

Alam, Ali Nazari; Sarvari, Jamal; Motamedifar, Mohammad; Khoshkharam, Hossein; Yousefi, Masoud; Moniri, Rezvan; Bazargani, Abdollah

doi:10.1016/j.genrep.2018.08.002

Cited by 8 publications

(5 citation statements)

References 28 publications

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“…The detection of blaSHV , and blaOXA genes in P . aeruginosa isolates aligns with findings from previous studies [ 4 , 48 , 49 ], further highlighting the significance of these genes in healthcare settings and their association with nosocomial infections. Their presence underscores the potential for these bacteria to cause severe infections and emphasizes the need for stringent infection control measures and judicious use of antibiotics to mitigate the emergence and spread of multidrug-resistant strains The difference between the results of this study and other investigations might be attributed to differences in various circumstances comprising incubators or to commonly occurring hyper-mutation among P .…”

Section: Discussionsupporting

confidence: 89%

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Islam,

Ferdous,

Hoque

et al. 2024

PLoS ONE

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The emergence and spread of multidrug-resistant pathogens like Pseudomonas aeruginosa are major concerns for public health worldwide. This study aimed to assess the prevalence of P. aeruginosa in clinical, environmental, and poultry sources in Bangladesh, along with their antibiotic susceptibility and the profiling of β-lactamase and virulence genes using standard molecular and microbiology techniques. We collected 110 samples from five different locations, viz., BAU residential area (BAURA; n = 15), BAU Healthcare Center (BAUHCC; n = 20), BAU Veterinary Teaching Hospital (BAUVTH; n = 22), Poultry Market (PM; n = 30) and Mymensingh Medical College Hospital (MCCH; n = 23). After overnight enrichment in nutrient broth, 89 probable Pseudomonas isolates (80.90%) were screened through selective culture, gram-staining and biochemical tests. Using genus- and species-specific PCR, we confirmed 22 isolates (20.0%) as P. aeruginosa from these samples. Antibiogram profiling revealed that 100.0% P. aeruginosa isolates (n = 22) were multidrug-resistant isolates, showing resistance against Doripenem, Penicillin, Ceftazidime, Cefepime, and Imipenem. Furthermore, resistance to aztreonam was observed in 95.45% isolates. However, P. aeruginosa isolates showed a varying degree of sensitivity against Amikacin, Gentamicin, and Ciprofloxacin. The blaTEM gene was detected in 86.0% isolates, while blaCMY, blaSHV and blaOXA, were detected in 27.0%, 18.0% and 5.0% of the P. aeruginosa isolates, respectively. The algD gene was detected in 32.0% isolates, whereas lasB and exoA genes were identified in 9.0% and 5.0% P. aeruginosa isolates. However, none of the P. aeruginosa isolates harbored exoS gene. Hence, this study provides valuable and novel insights on the resistance and virulence of circulating P. aeruginosa within the clinical, environmental, and poultry environments of Bangladesh. These findings are crucial for understanding the emergence of β-lactamase resistance in P. aeruginosa, highlighting its usefulness in the treatment and control of P. aeruginosa infections in both human and animal populations.

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Section: Discussionsupporting

confidence: 89%

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Islam,

Ferdous,

Hoque

et al. 2024

PLoS ONE

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“…Although, blaSHV , and blaOXA were identified as the less abundant ARG in P. aeruginosa isolates (18.0% and 4.55%, respectively), these ARGs were found in different combination. The detection of blaSHV , and blaOXA genes in P. aeruginosa isolates aligns with findings from previous studies [4, 47, 48], further highlighting the significance of these genes in healthcare settings and their association with nosocomial infections. Their presence underscores the potential for these bacteria to cause severe infections and emphasizes the need for stringent infection control measures and judicious use of antibiotics to mitigate the emergence and spread of multidrug-resistant strains The difference between the results of this study and other investigations might be attributed to differences in various circumstances comprising incubators or to commonly occurring hyper-mutation among P. aeruginosa strains exhibiting varied antibiotic resistance.…”

Section: Discussionsupporting

confidence: 89%

Characterization of β-lactamase and virulence genes inPseudomonas aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh

Islam,

Ferdous,

Hoque

et al. 2023

Preprint

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The emergence and spread of multidrug-resistant pathogens likePseudomonas aeruginosaare major concerns for public health worldwide. This study aimed to investigate the prevalence of circulatingP. aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh, their antibiotic susceptibility, β-lactamase and virulence gene profiling using standard molecular and microbiology techniques. We collected 110 samples from five different locations,viz., BAU residential area (BAURA; n=15), BAU Healthcare Center (BAUHCC; n = 20), BAU Veterinary Teaching Hospital (BAUVTH; n=22), Poultry Market (PM; n=30) and Mymensingh Medical College Hospital (MCCH; n=23). After overnight enrichment in nutrient broth, 89 probablePseudomonasisolates (80.90%) were screened through selective culture, gram-staining and biochemical tests. Using genus- and species-specific PCR, we confirmed 22 isolates (20.0%) asP. aeruginosafrom these samples. Antibiogram profiling revealed that 100.0%P. aeruginosaisolates (n = 22) were multidrug-resistant isolates, showing resistance against Doripenem, Penicillin, Ceftazidime, Cefepime, and Imipenem. Furthermore, resistance to aztreonam was observed in 95.45% isolates. However,P. aeruginosaisolates showed a varying degree of sensitivity against Amikacin, Gentamicin, and Ciprofloxacin. TheblaTEMgene was detected in 86.0% isolates, whileblaCMY,blaSHVandblaOXA,were detected in 27.0%, 18.0% and 5.0% of theP. aeruginosaisolates, respectively. ThealgDgene was detected in 32.0% isolates, whereaslasBandexoAgenes were identified in 9.0% and 5.0%P. aeruginosaisolates. However, none of theP. aeruginosaisolates harboredexoSgene. Thus, this study provides novel and important data on the resistance and virulence ofP. aeruginosacurrently circulating in clinical, environmental and poultry environment of Bangladesh. These data provide important insights into the emergence of β-lactamase resistance inP. aeruginosa, highlighting its usefulness in the treatment and control ofP. aeruginosainfections in both humans and animals.

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“…In other studies, the prevalence rate of MRSA was 32% among S. aureus isolates of dacryocystitis (23). Finally, the microbial profile may vary widely among geographic regions and during different periods (6). The prevalence of Gram-negative bacteria, including P. aeruginosa and Acinetobacter baumannii, and resistance to antibiotics were higher in this study than in other studies (10, 11).…”

Section: Discussioncontrasting

confidence: 52%

The Bacterial Profile and Microbial Susceptibility of Acute and Chronic Dacryocystitis in Matini Hospital, Kashan, Iran

Nazari-Alam

Badie

Shaeri

et al. 2021

Jundishapur J Microbiol

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Background: Recent studies have shown an increasing incidence of antibiotic resistance in dacryocystitis. Management of diseases may include determining microbial agents and choosing appropriate antibiotics for treatment. Objectives: This study aimed to present the best treatments for dacryocystitis. To this end, specimens' microbiology and antibiotic susceptibility were examined in patients with dacryocystitis in the microbiology laboratory of the Kashan University of Medical Sciences. Methods: This cross-sectional study was performed on 172 patients presenting with acute and chronic dacryocystitis at the Matini Hospital, Kashan, between 2017 - 2018. Patient characteristics, culture isolates, and antimicrobial susceptibility data were collected. The PCR assay of the mecA gene was performed in all methicillin-resistant Staphylococcus isolates. Results: The most common bacteria were coagulase-negative staphylococci, Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii. The majority of the isolated microbes were sensitive to rifampicin, linezolid, amikacin, and gentamicin. In Gram-negative bacilli, nine of the isolates were extended-spectrum beta-lactamase positive. The PCR test showed the frequency of mecA gene of resistant S. aureus and resistant coagulase-negative staphylococci (CoNS) isolates to be 40 and 46.3%, respectively. Conclusions: CoNS were the most frequently isolated bacteria. The highest antibiotic susceptibility was observed to rifampin, linezolid, amikacin, and gentamicin. A high percentage of CoNS carried the mecA gene.

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The occurrence of blaTEM, blaSHV and blaOXA genotypes in Extended-Spectrum β-Lactamase (ESBL)-producing Pseudomonas aeruginosa strains in Southwest of Iran

Cited by 8 publications

References 28 publications

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Characterization of β-lactamase and virulence genes inPseudomonas aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh

The Bacterial Profile and Microbial Susceptibility of Acute and Chronic Dacryocystitis in Matini Hospital, Kashan, Iran

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