The Occurrence of Chromosomal Aberrations in Pre-Spermatocytic Cells of Irradiated Male Mice. Iii. Sterility and Semisterility in the Offspring of Male Mice Irradiated in the Pre-Meiotic and Post-Meiotic Stages of Spermatogenesis

Griffen, A. B.; Bunker, Merrill C.

doi:10.1139/g67-020

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…In short, the irradiated parent of S-3611 became a permanent testicular mosaic for normal spermatocytes, for a population of spermatocytes carrying the translocation of S-3611, and for populations of spermatocytes carrying other aberrations. For any particular aberration such an individual is able to generate the entire gamut of normal and aneuploid sperm types and thus to sire aneuploid F1 animals directly; moreover, it is able to do so ti1roughout its reproductive life in cycle after cycle (Griffen and Bunker, 1967a As a means of clarifying the constitution of S-3611, a diagram was prepared, showing the con~plete segregational and disjunctional opportunities in a reciprocal translocation heterozygote or in a translocation area of a gonially mosaic testis at the first division of meiosis (Fig. 4).…”

Section: Discussionmentioning

confidence: 99%

“…149 davs after the deliverv of 700 r of acute X-rad;ation to the testes; therefore tke aberrant animal-was derived from irradiated T y p e A spermatogonia. (For con~plete details of the B N experiments see Griffen and Bunker, 1967a.). In mating opportunities which were maintained for a period of six months, with two changes of female mates.…”

Section: Methodsmentioning

confidence: 99%

“…1 A and B. In the preliminarv study of S-3611 (Griffen and Bunker, 1967a), only onc spermatogonial mitap~lask was observed, probably because the slides \vhic11 were madc permanent had been "scrcencd" and only those with abundant first meiotic metaphases retaincda practice which is not to be recommcnded. Since the singlc gonial cell scemed to have only 40 chromosomes, the slides wcrc again searched.…”

Section: Observationsmentioning

confidence: 99%

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A Case of Tertiary Trisomy in the Mouse, and Its Implications for the Cytological Classification of Trisomics in Other Mammals

Griffen¹

1967

Can. J. Genet. Cytol.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Observationsmentioning

confidence: 99%

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A Case of Tertiary Trisomy in the Mouse, and Its Implications for the Cytological Classification of Trisomics in Other Mammals

Griffen¹

1967

Can. J. Genet. Cytol.

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“…G riffen and Bunker (1967), studying the transmitted effects of both spermatogonial and postmeiotic germ-cell irradiation, found that 50% of the semisterile mice derived from exposed germ cells (350 r) possessed cytological anomalies. Doubling the dose increased the proportion to 61.5% as well as increased the frequency of semi sterility from 7.2% to 11.8%.…”

Section: Introductionmentioning

confidence: 99%

Chromosomal aberrations in cultured leukocytes from pigs derived from X-irradiated semen

Zartman¹,

Fechheimer²,

Baker³

1969

Cytogenet Genome Res

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Fresh semen from swine was exposed to 600 r of X-irradiation and used to inseminate sows. The chromosomes of the resultant liveborn progeny were studied through the use of leukocyte cultures. Of the 31 pigs studied, 4 had consistent large aberrations in all of the 30 cells studied per animal. Three of these aberrations were translocations; the other was a pericentric inversion. These were thought to be attributable to immediate effects of X rays. An interesting mosaic condition was seen in three of the pigs studied. It was apparently inherited, at least in two of them. All of the pigs had a breakage-deletion condition for the same chromosome. Among the delayed effects of X-irradiation were chromosome alterations including aneuploidy, fragments, chromosome breaks, chromatid breaks and chromosome gaps. Other traits studied were chromatid gaps, secondary constrictions, pericentric inversions and sister chromatid fusions. The independent variables included in the analyses of variance were X-irradiation, breed, sex, irradiation × sex interaction and breed × sex interaction. The effects of these independent variables were not lucid, but there were some conclusions drawn. X-irradiation had a threshold effect, with the effect being a suppression of aberrations. Females, when interacting with the irradiation or breed effects, had higher frequencies of aberrations than males. Breed effects were not consistent, and no trend was apparent. Of the nine dependent variables studied, only fragments and chromosome breaks were noticeably correlated.

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New approaches to mutagenicity studies in animals for carcinogenic and mutagenic agents. I. Modification of the heritable translocation test

Adler¹

1981

Teratog Carcinog Mutagen

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Two alternative modifications of the experimental protocol for the heritable translocation test are described. One of them proposes to mate F1 males and F1 females within one experimental group and eliminate normal pairs by a sequential decision procedure based on litter sizes. Pairs that do not meet the criteria for normal litter size have to be separated and tested against normal partners. Male translocation suspects are analyzed cytogenetically for presence of a reciprocal translocation. Female translocation suspects or XO suspects are verified through analysis of their male and female progeny. The second modification of the heritable translocation test omits fertility testing and proposes to cytogenetically analyze 25 diakineses-metaphases I from each F1 male in the test. Using either one or both protocols it was shown that 20 mg/kg of methyl methanesulfonate (MMS) induced 1.3% heritable translocations in late spermatids and early spermatozoa. With 2.5 mg/kg of mitomycin C, 0.3% and 0.4% translocation carriers were recovered from treated primary spermatocytes and early spermatids, respectively. A dose of 300 R gamma rays resulted in 1.6% translocations in spermatozoa and 4.8% translocations in primary spermatocytes. The advantage of the modified fertility test lies in the doubling of the sample size by including the females. However, a disadvantage is the amount of time and labor that is necessary to verify female translocation carriers. The cytogenetic translocation test protocol with all F1 males is considered more reliable and faster. It lacks the possibility of error entailed in the decision procedure by fertility testing, and it requires less time, personnel, and animal space.

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The Occurrence of Chromosomal Aberrations in Pre-Spermatocytic Cells of Irradiated Male Mice. Iii. Sterility and Semisterility in the Offspring of Male Mice Irradiated in the Pre-Meiotic and Post-Meiotic Stages of Spermatogenesis

Cited by 11 publications

References 16 publications

A Case of Tertiary Trisomy in the Mouse, and Its Implications for the Cytological Classification of Trisomics in Other Mammals

A Case of Tertiary Trisomy in the Mouse, and Its Implications for the Cytological Classification of Trisomics in Other Mammals

Chromosomal aberrations in cultured leukocytes from pigs derived from X-irradiated semen

New approaches to mutagenicity studies in animals for carcinogenic and mutagenic agents. I. Modification of the heritable translocation test

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