The occurrence of cryoproteins in synovial fluid; the association of a complement-fixing activity in rheumatoid synovial fluid with cold-precipitable protein

Marcus, Robert L.; Townes, Alexander S.

doi:10.1172/jci106493

Cited by 39 publications

(20 citation statements)

References 32 publications

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“…Reactions of partial identity were observed with SDNA. Cryoproteins obtained from rheumatoid as well as nonrheumatoid synovial fluid have been shown to contain DNA (11,12). No association between the presence of DNA and anti-NDNA or anti-SDNA antibody in the cryoproteins has been demonstrated.…”

Section: Incidence Of Sdna Antigen In Various Diseasesmentioning

confidence: 96%

The Occurrence of Single-Stranded DNA in the Serum of Patients with Systemic Lupus Erythematosus and Other Diseases

Koffler¹,

Agnello²,

Winchester³

et al. 1973

J. Clin. Invest.

224

103

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Section: Incidence Of Sdna Antigen In Various Diseasesmentioning

confidence: 96%

The Occurrence of Single-Stranded DNA in the Serum of Patients with Systemic Lupus Erythematosus and Other Diseases

Koffler¹,

Agnello²,

Winchester³

et al. 1973

J. Clin. Invest.

224

103

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“…Secondly, methods which are not specific for any particular antigen-antibody system were used. These studies were mostly qualitative and led to the demonstration of immune complex-like material such as cryoglobulins (16,17), macromolecular complexes (3,10,11), Clq precipitins (4,5), complement fixing material (4,16), or of complexes reacting with monoclonal rheumatoid factor (5). Recently, quantitative investigations were carried out using radioimmunological techniques such as for binding of complexes by rheumatoid factor (18,19) and macrophages (20).…”

Section: Introductionmentioning

confidence: 99%

Circulating and intra-articular immune complexes in patients with rheumatoid arthritis. Correlation of 125I-Clq binding activity with clinical and biological features of the disease.

Zubler¹,

Nydegger²,

Perrin³

et al. 1976

J. Clin. Invest.

246

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“…In rheumatoid arthritis (RA),' cryoglobulins (18,19) and immune complexes (20) are found in synovial fluid and these are thought to play a role in the pathogenesis of the articular inflammation that occurs in that disease (21). In the sera of such patients, however, immune complexes are only occasionally demonstrated (22), and spontaneous precipitates are infrequently recognized in refrigerated rheumatoid sera (23,24).…”

Section: Introductionmentioning

confidence: 99%

Cryoimmunoglobulinemia in rheumatoid arthritis. Significance in serum of patients with rheumatoid vasculitis.

Weisman¹,

Zvaifler²

1975

J. Clin. Invest.

113

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A B S T R A C T Cryoglobulins were examined in a standardized manner in an unselected group of 35 patients with rheumatoid arthritis (RA) and 8 patients with RA complicated by cutaneous vasculitis and neuropathy. Optimum conditions for detection and characterization of cryoglobulins were established; the proportion of resolubilized to total precipitable protein remained constant in an individual patient under these conditions. All 8 vasculitis patients and 9 of 35 other patients with RA exhibited cryoglobulins; total protein and immunoglobulin content were significantly higher in the cryoglobulins of patients with vasculitis. Immunoglobulins G and M constituted two-thirds and three-quarters of the total protein in the cryoglobulins from uncomplicated rheumatoid and vasculitis patients, respectively. Serum antiglobulin titers were higher, and serum C3 levels were lower, in vasculitis patients compared to rheumatoid patients without vasculitis.Anti-gamma globulin activity was detected in all cryoglobulins from vasculitis patients. Cryoglobulin IgG and IgM were polyclonal. Density gradient analyses demonstrated the majority of the cryoglobulin antiglobulin activity to reside in the 19S IgM fraction. There was no evidence of a light weight (8S) IgM (2). Fourteen years after the original observation a patient was described who exhibited a similar phenomenon in the absence of malignancy and whose clinical features suggested an immunologically mediated disease (3). In recent years the emphasis of investigations concerning these proteins has changed. Now they are being viewed as circulating immune complexes because of the demonstration that they contain appropriate antigens, antibodies, or other immunoreactants that may relate to the pathogenesis of the disease they accompany (4). For example, cryoglobulins occur regularly in the sera of patients with diseases of known infectious etiology associated with "autoimmune" features, such as infectious mononucleosis (5, 6), leprosy (7), poststreptococcal glomerulonephritis (8), cytomegalovirus syndromes (6, 9), subacute bacterial endocarditis (10, 11), and tropical splenomegaly syndrome, resulting from chronic malarial exposure (12 METHODSPatients. Samples were obtained from 38 patients with RA, who were encountered in follow-up visits to a rheumatology clinic or hospitalized for either orthopedic or medical complications of their disease. They were chosen at random over a 6-mo period (January-June 1972). Patients with rheumatoid vasculitis were chosen consecutively as they were identified over a 12-mo period (January-December 1972). Patients with RA were representative of a total population of clinic-based rheumatoids; patients with vasculitis were either discovered in our clinic based population (W. H.), referred to our rheumatology division for evaluation of vasculitis (J. S., D. A., M. H., and L. T.), or hospitalized at the Veterans Administration Hospital for vasculitis (D. M., J. S., and R. M.). All patients (including those with vasculitis) met the American Rheumati...

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The occurrence of cryoproteins in synovial fluid; the association of a complement-fixing activity in rheumatoid synovial fluid with cold-precipitable protein

Cited by 39 publications

References 32 publications

The Occurrence of Single-Stranded DNA in the Serum of Patients with Systemic Lupus Erythematosus and Other Diseases

The Occurrence of Single-Stranded DNA in the Serum of Patients with Systemic Lupus Erythematosus and Other Diseases

Circulating and intra-articular immune complexes in patients with rheumatoid arthritis. Correlation of 125I-Clq binding activity with clinical and biological features of the disease.

Cryoimmunoglobulinemia in rheumatoid arthritis. Significance in serum of patients with rheumatoid vasculitis.

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