The occurrence of cyclic AMP in archaebacteria

Leichtling, Ben H.; Rickenberg, H.V.; Seely, Robert J.; Fahrney, David E.; Pace, Norman R.

doi:10.1016/0006-291x(86)90443-2

Cited by 20 publications

(16 citation statements)

References 12 publications

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“…Moreover, many of these are components of likely transmembrane receptor proteins [56]. cAMP has thus far been detected in four phylogenetically and metabolically diverse members of the Archaea: Halobacterium salinarium [58], Halobacterium volcanii [59]. Methanobacterium thermoautotrophicum [58], and S. solfataricus [59].…”

Section: Resultsmentioning

confidence: 99%

“…cAMP has thus far been detected in four phylogenetically and metabolically diverse members of the Archaea: Halobacterium salinarium [58], Halobacterium volcanii [59]. Methanobacterium thermoautotrophicum [58], and S. solfataricus [59]. The basal concentration of 3ʹ,5ʹ-cAMP in H. salinarium, which oscillates during the cell division cycle, has been estimated to be 200 µM, while the quantity in S. solfataricus was reported as ~ 110 pmol/mg protein.…”

Section: Resultsmentioning

confidence: 99%

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Activation of SsoPK4, an Archaeal eIF2α Kinase Homolog, by Oxidized CoA

et al. 2015

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The eukaryotic protein kinase (ePK) paradigm provides integral components for signal transduction cascades throughout nature. However, while so-called typical ePKs permeate the Eucarya and Bacteria, atypical ePKs dominate the kinomes of the Archaea. Intriguingly, the catalytic domains of the handful of deduced typical ePKs from the archaeon Sulfolobus solfataricus P2 exhibit significant resemblance to the protein kinases that phosphorylate translation initiation factor 2α (eIF2α) in response to cellular stresses. We cloned and expressed one of these archaeal eIF2α protein kinases, SsoPK4. SsoPK4 exhibited protein-serine/threonine kinase activity toward several proteins, including the S. solfataricus homolog of eIF2α, aIF2α. The activity of SsoPK4 was inhibited in vitro by 3ʹ,5ʹ-cyclic AMP (Ki of ~23 µM) and was activated by oxidized Coenzyme A, an indicator of oxidative stress in the Archaea. Activation enhanced the apparent affinity for protein substrates, Km, but had little effect on Vmax. Autophosphorylation activated SsoPK4 and rendered it insensitive to oxidized Coenzyme A.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Activation of SsoPK4, an Archaeal eIF2α Kinase Homolog, by Oxidized CoA

et al. 2015

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“…A very early report revealed that cAMP occurs also in three archaeal species, and it was speculated that it might be a signal for carbon starvation, similar to its role in E. coli [33]. But this result was not followed up and the distribution of cAMP in archaea and its functional role remained unknown.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome changes and cAMP oscillations in an archaeal cell cycle

2007

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Background: The cell cycle of all organisms includes mass increase by a factor of two, replication of the genetic material, segregation of the genome to different parts of the cell, and cell division into two daughter cells. It is tightly regulated and typically includes cell cycle-specific oscillations of the levels of transcripts, proteins, protein modifications, and signaling molecules. Until now cell cycle-specific transcriptome changes have been described for four eukaryotic species ranging from yeast to human, but only for two prokaryotic species. Similarly, oscillations of small signaling molecules have been identified in very few eukaryotic species, but not in any prokaryote.

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“…However, surprisingly little is known about the presence and molecular function of cyclic nucleotides in archaea. In 1986, the presence of cAMP was reported in the archaeal species Methanobacterium thermautotrophicus, Sulfolobus solfataricus, and Haloferax volcanii (then referred to as Halobacterium volcanii) (Leichtling, Rickenberg, Seely, Fahrney, & Pace, 1986), and cAMP levels have also been investigated in Halobacterium salinarum where a cell cycle-dependent fluctuation of the intracellular cAMP concentration has been observed (Baumann, Lange, & Soppa, 2007). Another cyclic nucleotide that has been characterized in archaea is cyclic oligoadenylate, which has been shown to be involved in type III clustered regularly interspaced short palindromic repeats (CRISPR)-mediated immunity in S. solfataricus (Rouillon, Athukoralage, Graham, Grüschow, & White, 2018).…”

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confidence: 99%

Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeonHaloferax volcaniiand its putative role

et al. 2019

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The role of cyclic nucleotides as second messengers for intracellular signal transduction has been well described in bacteria. One recently discovered bacterial second messenger is cyclic di‐adenylate monophosphate (c‐di‐AMP), which has been demonstrated to be essential in bacteria. Compared to bacteria, significantly less is known about second messengers in archaea. This study presents the first evidence of in vivo presence of c‐di‐AMP in an archaeon. The model organism Haloferax volcanii was demonstrated to produce c‐di‐AMP. Its genome encodes one diadenylate cyclase (DacZ) which was shown to produce c‐di‐AMP in vitro. Similar to bacteria, the dacZ gene is essential and homologous overexpression of DacZ leads to cell death, suggesting the need for tight regulation of c‐di‐AMP levels. Such tight regulation often indicates the control of important regulatory processes. A central target of c‐di‐AMP signaling in bacteria is cellular osmohomeostasis. The results presented here suggest a comparable function in H. volcanii. A strain with decreased c‐di‐AMP levels exhibited an increased cell area in hypo‐salt medium, implying impaired osmoregulation. In summary, this study expands the field of research on c‐di‐AMP and its physiological function to archaea and indicates that osmoregulation is likely to be a common function of c‐di‐AMP in bacteria and archaea.

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The occurrence of cyclic AMP in archaebacteria

Cited by 20 publications

References 12 publications

Activation of SsoPK4, an Archaeal eIF2α Kinase Homolog, by Oxidized CoA

Activation of SsoPK4, an Archaeal eIF2α Kinase Homolog, by Oxidized CoA

Transcriptome changes and cAMP oscillations in an archaeal cell cycle

Cyclic nucleotides in archaea: Cyclic di‐AMP in the archaeonHaloferax volcaniiand its putative role

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