The occurrence of enterotoxigenic isolates of B. cereus in foodstuffs

Abstract

Brychta J., Smola J., Pipek P., Ondráček J., Bednář V., Čížek A., Brychta T. (2009): The occurrence of enterotoxigenic isolates of B. cereus in foodstuffs. Czech J. Food Sci., 27: 284-292.Enterotoxigenic Bacillus cereus was detected in a variety of meat stuffs (36), ready-to-cook products (5), and swabs (7). The bacterial colonies isolated from PEMBA agar were identified as B. cereus. The 85 isolates were examined for the enterotoxin production using both TECRA-VIA and BCET-RPLA kits (ELISA -47, RPLA -38). Thirty two isolates (66%) were positive for enterotoxin using the ELISA test while only 15 isolates (39%) gave positive results in the RPLA test system. In total, 178 (91.8%) and 164 (84%) of the strains isolated in our laboratory (from various foods) were enterotoxigenic as determined using TECRA-VIA and BCET-RPLA, respectively. Parallel enterotoxin positive results obtained using both tests were demonstrated in only 9 isolates from 19 assessed (47.4%). Coincidental negative results from both kits were established for 3 isolates (15.8%) only. The isolates of B.cereus from meat were resistant to cephalothin (57%), clindamycin (14%), oxytetracycline (14%), and erythromycin (7%). The isolates from swabs were resistant to cephalothin (83%), erythromycin (16%), clindamycin (16%) and enrofloxacin (16%).

Genomic Diversity and Virulence Genes Characterization of Bacillus cereus sensu lato Isolated from Processing Equipment of an Algerian Dairy Plant

“…Te existence of B. cereus in dairy plants can be attributed to diferent sources such as the cow udders, collecting containers, transportation systems, and milk powder in the case of recombined milk [60]. Teir presence in the processing equipment demonstrates that the cleaning and disinfection procedures have not been performed carefully on the production equipment [61] or that the methods and products used are not efcient enough. In a previous study, the spores of the isolates herein investigated were shown to have diferent lengths of appendages, the surface of the exosporium and spore hydrophobicity which enable these spores to adhere to stainless steel surfaces [32].…”

Detection and Determination of Bacillus cereus in Cooked Rice and Some Types of Spices with Ribosomal 16SrRNA gene Selected from Iraqi Public Restaurants

“…This would clearly indicate that bacillus having specific phenotypic line. According to the above described methods, an isolated Bacillus strains, are identified for each strain separately with Ribosomal 16SrRNA gene-specific PCR, which allowed for seven strains (indicated in Figure 1) to be identified compare with molecular marker determined as B. cereus (Brychta et al, 2009;Beecher and Wong, 1994). From the group of Bacillus spp.…”

“…Emetic B. cereus is presumably unable to grow and produce their toxin cereulide below 10°C, or in the absence of oxygen (EFSA, 2005). The (ELISA) Enzyme linked immunosorbent Adsorbed, is an immunoreactions which can use in toxins detections, and these types is useful in certain strains of bacteria to detect toxins (Brychta et al, 2009). The genotypic and phenotypic heterogeneities of the genus Bacillus, evident already for a long time (Ash et al, 1993).…”